公开(公告)号：US20190127741A1

公开(公告)日：2019-05-02

申请号：US16143189

申请日：2018-09-26

Applicant: Genentech, Inc.

Inventor： Laetitia MALPHETTES ,  Andrew Snowden ,  Inn H. Yuk

IPC: C12N15/67 ,  C12N15/63

Abstract: The invention provides compositions and methods for regulating intracellular osmolarity in cells, e.g., in cultured cells, including in cultured cells in bioreactors. The invention provides nucleic acids comprising at least one osmo-responsive transcriptional regulatory element (OR-TRE), and cells, vectors, products of manufacture, artificial organs or implants and the like containing an osmo-responsive transcriptional regulatory element (OR-TRE).

公开(公告)号：US20150307888A1

公开(公告)日：2015-10-29

申请号：US14549962

申请日：2014-11-21

Applicant: Genentech, Inc.

Inventor： Laetitia MALPHETTES ,  Andrew SNOWDEN ,  Inn H. YUK

IPC: C12N15/67

CPC classification number: C12N15/67 ,  C12N15/63 ,  C12N2830/002

Abstract: The invention provides compositions and methods for regulating intracellular osmolarity in cells, e.g., in cultured cells, including in cultured cells in bioreactors. The invention provides nucleic acids comprising at least one osmo-responsive transcriptional regulatory element (OR-TRE), and cells, vectors, products of manufacture, artificial organs or implants and the like containing an osmo-responsive transcriptional regulatory element (OR-TRE).

Abstract translation: 本发明提供用于调节细胞中的细胞内渗透压的组合物和方法，例如在培养的细胞中，包括在生物反应器中的培养细胞中。 本发明提供包含至少一种渗透应答转录调控元件（OR-TRE）的核酸，以及含有渗透应答转录调节元件（OR-TRE）的细胞，载体，制造产物，人造器官或植入物等， 。