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公开(公告)号：US11966086B2
公开(公告)日：2024-04-23
申请号：US17830115
申请日：2022-06-01
发明人： David Buse , David Howard Combs , Norbert D. Hagen , David Opalsky , Bruce Richardson , Anita Prasad , Keith Moravick , Tyler Moore
CPC分类号： G02B6/3668 , G01N21/253 , G01N21/6452 , G02B6/06 , G02B6/08 , G02B6/3672 , G02B6/4214 , G02B6/4246 , G02B6/4269 , G01N2021/6484
摘要： A first time/temperature data set includes temperatures and time stamps recorded at time intervals during a first thermal cycling process and a time stamp for each time interval, and a second time/temperature data set includes temperatures and time stamps recorded at time intervals during a second thermal cycling process. Signal emissions at different signal detecting positions are sequentially measured at different time intervals to generate first and second time/signal emission data sets for receptacles subject to the first and second thermal cycling processes, respectively. Signal emissions of receptacles subject to the first thermal cycling process are synchronized with specific temperatures of the first thermal cycling process by comparing time stamps of the first time/signal emission data set for each receptacle with the time stamps of the first time/temperature data set that correspond with the specific temperature. Signal emissions of receptacles subject to the second thermal cycling process are synchronized with specific temperatures of the second thermal cycling process by comparing time stamps of the second time/signal emission data set for each receptacle with the time stamps of the second time/temperature data set that correspond with the specific temperature.
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公开(公告)号：US11840727B2
公开(公告)日：2023-12-12
申请号：US17028367
申请日：2020-09-22
发明人： David Buse , David Howard Combs , Norbert D. Hagen , David Opalsky , Bruce Richardson , Anita Prasad , Keith Moravick , Tyler Moore , Byron J. Knight
CPC分类号： C12Q1/686 , B01L7/52 , G01N21/6452 , B01L9/06 , B01L2200/141 , B01L2200/142 , B01L2300/042 , B01L2300/0654 , B01L2300/0829 , B01L2300/0851 , B01L2300/1822 , G01N2021/6482 , G01N2021/6484
摘要： Multiple receptacles containing a reaction mixture are transported to corresponding receptacle wells of a thermally-conductive receptacle holder in thermal communication with a support that is in thermal communication with a heat sink. The heat sink is pre-heated to a temperature above ambient temperature, and the temperature of the receptacle holder is cycled. Optical communication is established between each of the receptacle wells and an excitation signal source and an emission signal detector during cycling, and whether an emission signal is emitted from any of the receptacles is determined as an indication of the presence of a target nucleic acid.
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公开(公告)号：US11746373B2
公开(公告)日：2023-09-05
申请号：US17847096
申请日：2022-06-22
发明人： Byron J. Knight , David A. Buse , Norbert D. Hagen , David Opalsky , Tyler Moore , Anita Prasad , Bruce Richardson
IPC分类号： B01L3/00 , B01L7/00 , G01N35/04 , C12Q1/6806 , C12Q1/686
CPC分类号： C12Q1/6806 , B01L3/50825 , B01L3/565 , B01L7/5255 , C12Q1/686 , G01N35/04 , B01L3/527 , B01L2200/026 , B01L2200/0689 , B01L2300/046 , G01N2035/0418 , G01N2035/0436
摘要： A cap and a processing vial configured for interlocking engagement. The processing vial includes a locking collar surrounding an open upper end of the processing vial with lateral through holes formed through the locking collar. The vial also includes a latch hook located above each through hole. The cap includes a latch collar extending about a lower portion of the cap, where the latch collar is configured to snap in beneath the latch hooks of the processing vial when the lower portion of the cap is inserted into the open upper end of the processing vial to lock the cap to the processing vial.
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公开(公告)号：US20220291454A1
公开(公告)日：2022-09-15
申请号：US17830115
申请日：2022-06-01
发明人： David Buse , David Combs , Norbert D. Hagen , David Opalsky , Bruce Richardson , Anita Prasad , Keith Moravick , Tyler Moore
摘要： A first time/temperature data set includes temperatures and time stamps recorded at time intervals during a first thermal cycling process and a time stamp for each time interval, and a second time/temperature data set includes temperatures and time stamps recorded at time intervals during a second thermal cycling process. Signal emissions at different signal detecting positions are sequentially measured at different time intervals to generate first and second time/signal emission data sets for receptacles subject to the first and second thermal cycling processes, respectively. Signal emissions of receptacles subject to the first thermal cycling process are synchronized with specific temperatures of the first thermal cycling process by comparing time stamps of the first time/signal emission data set for each receptacle with the time stamps of the first time/temperature data set that correspond with the specific temperature. Signal emissions of receptacles subject to the second thermal cycling process are synchronized with specific temperatures of the second thermal cycling process by comparing time stamps of the second time/signal emission data set for each receptacle with the time stamps of the second time/temperature data set that correspond with the specific temperature.
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公开(公告)号：US11788128B2
公开(公告)日：2023-10-17
申请号：US17029930
申请日：2020-09-23
发明人： David Buse , David Howard Combs , Norbert D. Hagen , David Opalsky , Bruce Richardson , Anita Prasad , Keith Moravick , Tyler Moore
CPC分类号： C12Q1/686 , B01L7/52 , G01N21/6452 , B01L9/06 , B01L2200/141 , B01L2200/142 , B01L2300/042 , B01L2300/0654 , B01L2300/0829 , B01L2300/0851 , B01L2300/1822 , G01N2021/6482 , G01N2021/6484
摘要： An apparatus for performing nucleic acid amplification reactions includes a thermally-conductive receptacle holder with front and back surfaces. The front surface includes a row of receptacle wells, and an outer surface of each well has a frustoconical shape, an inner surface of each well receives a receptacle, and each well has a through-hole extending between the inner and outer surfaces thereof. One or more thermal elements in contact with the back surface of the holder alter a temperature(s) of the holder. The apparatus includes multiple optical fibers, and each optical fiber provides optical communication between one of the wells and an excitation signal source and/or an emission signal detector. A first end of each optical fiber is disposed outside, within, or extends through the through-hole of a corresponding well, and a second end of each optical fiber is in optical communication with the excitation signal source and/or the emission signal detector.
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公开(公告)号：US11434521B2
公开(公告)日：2022-09-06
申请号：US17668775
申请日：2022-02-10
发明人： David A. Buse , David Howard Combs , Byron J. Knight , Norbert D. Hagen , David Opalsky , Jason F. Rhubottom , Keith Moravick , Tyler Moore , Anita Prasad , Bruce Richardson
IPC分类号： C12Q1/6806 , G01N35/04 , B01L3/00 , B01L7/00 , C12Q1/686
摘要： A method for performing a nucleic acid amplification assay employs a thermally-conductive receptacle holder with one or more receptacle wells, each conforming to an outer surface of a lower portion of a vial. A through-hole extends from an inner surface of each well to an outer surface of the holder. A thermal element is positioned proximal to the holder for altering a temperature of the holder. A signal detection module is configured to generate an excitation signal directed through the through-hole of the well and detect an optical emission from a fluid contained in the vial supported by the well. At least one well supports a capped vial containing a reagent for a nucleic acid amplification assay and including an opaque cap sealing an open end of the vial. The lower portion of the vial is contained within a well, and the cap is situated above a top surface of the holder.
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公开(公告)号：US11279967B2
公开(公告)日：2022-03-22
申请号：US17036730
申请日：2020-09-29
发明人： David A. Buse , David Howard Combs , Byron J. Knight , Norbert D. Hagen , David Opalsky , Jason F. Rhubottom , Keith Moravick , Tyler Moore , Anita Prasad , Bruce Richardson
IPC分类号： C12Q1/6806 , G01N35/04 , B01L3/00 , B01L7/00 , C12Q1/686
摘要： A system for performing a nucleic acid amplification assay that includes a receptacle holder formed from a thermally conductive material and having a receptacle well for receiving a lower portion of a vial; a thermal element for cycling the temperature of the receptacle holder; a signal detection module in optical communication with the receptacle well; and a robotic pipettor. The receptacle holder supports a capped vial having a plastic, opaque cap in a snap-fit with a single plastic vial containing a nucleic acid amplification reagent. An upper portion of the cap includes a probe recess that is configured to be removably engaged by an end of a probe of the robotic pipettor. A lower portion of the vial is contained within the receptacle well, and the cap is situated above a top surface of the receptacle holder.
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公开(公告)号：US10961572B2
公开(公告)日：2021-03-30
申请号：US16434910
申请日：2019-06-07
发明人： David Buse , David Howard Combs , Norbert D. Hagen , David Opalsky , Bruce Richardson , Anita Prasad , Keith Moravick , Tyler Moore
摘要： A thermally conductive receptacle holder includes a plurality of wells that each receive a receptacle. A cross-brace is mounted to a support to exert a force onto an outer surface of a compressive housing such that the compressive housing exerts a force onto the receptacle holder. An optical fiber associated with each well provides optical communication between the receptacle well and an excitation signal source and/or an emission signal detector.
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公开(公告)号：US20210017579A1
公开(公告)日：2021-01-21
申请号：US17036730
申请日：2020-09-29
发明人： David A. Buse , David Howard Combs , Byron J. Knight , Norbert D. Hagen , David Opalsky , Jason F. Rhubottom , Keith Moravick , Tyler Moore , Anita Prasad , Bruce Richardson
IPC分类号： C12Q1/6806 , G01N35/04 , B01L3/00 , B01L7/00 , C12Q1/686
摘要： A system for performing a nucleic acid amplification assay comprises a thermally-conductive receptacle holder with one or more receptacle wells, each conforming to an outer surface of a lower portion of a vial. A through-hole extends from an inner surface of each well to an outer surface of the holder. A thermal element is positioned proximal to the holder for altering a temperature of the holder. A signal detection module is configured to generate an excitation signal directed through the through-hole of the well and detect an optical emission from a fluid contained in the vial supported by the well. At least one well supports a capped vial containing a reagent for a nucleic acid amplification assay and including an opaque cap sealing an open end of the vial. The lower portion of the vial is contained within a well, and the cap is situated above a top surface of the holder.
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公开(公告)号：US11788115B2
公开(公告)日：2023-10-17
申请号：US17033395
申请日：2020-09-25
发明人： David Buse , Norbert D. Hagen , David Opalsky , Bruce Richardson , Anita Prasad , Tyler Moore , Byron J. Knight
IPC分类号： C12Q1/6806 , G01N35/04 , B01L3/00 , B01L7/00 , C12Q1/686
CPC分类号： C12Q1/6806 , B01L3/50825 , B01L3/565 , B01L7/5255 , C12Q1/686 , G01N35/04 , B01L3/527 , B01L2200/026 , B01L2200/0689 , B01L2300/046 , G01N2035/0418 , G01N2035/0436
摘要： A receptacle cap includes a probe recess configured to receive a probe inserted therein to thereby frictionally secure the receptacle cap to the probe, and a closed lower end of the receptacle is insertable into an open end of a reaction receptacle to close the reaction receptacle. The receptacle cap and the reaction receptacle include interlocking features for securing the receptacle cap to the reaction receptacle.
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