公开(公告)号：US20190142311A1

公开(公告)日：2019-05-16

申请号：US16163011

申请日：2018-10-17

Applicant: Eccrine Systems, Inc.

Inventor： Jason C. Heikenfeld ,  Mikel Larson ,  Florika Macazo ,  Jacob A. Bertrand

IPC: A61B5/145 ,  A61B5/1495 ,  A61B5/00

Abstract: Devices for calibrating electrochemical aptamer-based biofluid sensors. In some embodiments a calibrant is located proximate to the EAB sensors, and calibration is facilitated by introduction of the biofluid into the device. Other embodiments include various mechanisms for introducing calibration solution to the sensors, including drawing calibration solution into contact with sensors through electrical potential, a dispensing mechanism such as an ink jet-type nozzle, as well as pressure actuated calibration dispensing. Also included are embodiments employing bifurcated biofluid transport paths that allow alternately for calibration and biofluid sensing.

公开(公告)号：US20180256137A1

公开(公告)日：2018-09-13

申请号：US15769435

申请日：2016-10-23

Applicant: Eccrine Systems, Inc.

Inventor： Jason Heikenfeld ,  Hector Wong ,  Kevin Plaxco ,  Jacob A. Bertrand

IPC: A61B10/00 ,  G01N33/487 ,  A61B5/145 ,  A61B5/1477 ,  A61B5/00 ,  G01N27/327 ,  G01N27/40

CPC classification number: A61B10/0064 ,  A61B5/14521 ,  A61B5/14539 ,  A61B5/14546 ,  A61B5/1477 ,  A61B5/4266 ,  A61B5/6802 ,  A61B5/6803 ,  A61B5/6804 ,  A61B2562/04 ,  A61B2562/16 ,  G01N27/3276 ,  G01N27/40 ,  G01N33/48707 ,  G01N33/50

Abstract: Embodiments of the disclosed invention provide devices and methods for buffering fluid samples to enable accurate concentration measurements of analytes by salinity-sensitive or pH-sensitive sensors. The buffering capabilities of the device include the ability to control the salinity and pH of a fluid sample, specifically, through the management of solutes such as salts, H+, other ions, and other solutes, that are found in sweat, biofluids, or other fluids. The purpose of such control is to enhance particular fluid sensing device applications by improving detectability of the targeted analyte, or improving performance of analyte sensors. Some embodiments also include components to enable sample concentration to enhance the measurement of low-concentration solutes found in the fluid.

公开(公告)号：US20180020966A1

公开(公告)日：2018-01-25

申请号：US15653494

申请日：2017-07-18

Applicant: Eccrine Systems, Inc.

Inventor： Gavi Begtrup ,  Jacob A. Bertrand ,  Jason Heikenfeld ,  Austin Morgan ,  Nathan Weinle

IPC: A61B5/00 ,  A61B5/145 ,  A61B5/01 ,  A61B5/053

CPC classification number: A61B5/4266 ,  A61B5/01 ,  A61B5/0531 ,  A61B5/0533 ,  A61B5/14517 ,  A61B5/14539 ,  A61B5/14546 ,  A61B5/6801 ,  A61B5/7275 ,  A61B2503/04 ,  A61B2503/10 ,  A61B2562/0219 ,  A61B2562/14

Abstract: The disclosed invention includes sweat sensing devices configured to periodically measure sweat conductivity and galvanic skin response, devices to measure volumetric sweat flow rate, and devices that combine the three functions. The disclosure further includes methods for using a device configured to perform periodic sweat conductivity measurements, galvanic skin response measurements, and volumetric sweat rate measurements so that each sensor modality informs composite estimates of sweat onset, sweat cessation, sweat ion concentration, and sweat rate. The method uses those measurements to inform other sweat sensing device functions, such as determining the existence of a physiological condition, or performing measurements of concentrations, ratios, and trends of sweat analytes.

公开(公告)号：US20210204840A1

公开(公告)日：2021-07-08

申请号：US16087342

申请日：2017-03-21

Applicant: Eccrine Systems, Inc.

Inventor： Jacob A. Bertrand ,  Gavi Begtrup ,  Leila Safazadeh Haghighi ,  Jason Heikenfeld

IPC: A61B5/1477 ,  G01N33/543 ,  A61B5/145 ,  A61B5/01 ,  A61B5/0533 ,  A61B5/00

Abstract: Challenges of the sweat medium for electrochemical aptamer-based biosensor (“EAB sensor”) devices can be mitigated through aptamer selection, sensor/device configuration and physiological algorithms that account for the effects of (1) target analyte size, (2) potential concentration ranges, (3) sweat sample pH, and (4) sweat sample salinity. The disclosed invention includes a method of aptamer selection for use in an EAB sensor configured for use in a wearable sweat sensing device. The disclosed invention further provides a sweat sensing device configured to use EAB sensors to detect target analytes in a sweat sample.

公开(公告)号：US10506968B2

公开(公告)日：2019-12-17

申请号：US15966476

申请日：2018-04-30

Applicant: Eccrine Systems, Inc. ,  University of Cincinnati

Inventor： Jason Heikenfeld ,  Jacob A. Bertrand ,  Michael Brothers ,  Andrew Jajack

IPC: A61B5/145 ,  A61B5/00 ,  G01N33/50 ,  A61B10/00 ,  A61B5/1468 ,  A61F13/02 ,  G01N33/66 ,  G01N33/53 ,  G01N33/543

Abstract: The disclosed invention provides a fluid sensing device and method capable of collecting a fluid sample, concentrating the sample with respect to one or more target analytes, and measuring the target analyte(s) in the concentrated sample. The invention is also capable of determining the change in molarity of the fluid sample with respect to the target analyte(s), as the sample is concentrated by the device. The invention further includes a method for using a fluid sensing device to concentrate a fluid sample with respect to one or more target analytes. The disclosed method further includes the ability to correlate the measured target analyte concentration to a physiological condition of a device wearer, or of a fluid source.

公开(公告)号：US20200155046A1

公开(公告)日：2020-05-21

申请号：US16484989

申请日：2018-02-07

Applicant: Eccrine Systems, Inc.

Inventor： Robert Beech ,  Gavi Begtrup ,  Jason Heikenfeld ,  Jacob A. Bertrand

IPC: A61B5/145 ,  A61B5/01 ,  A61B5/00 ,  A61B5/1477

Abstract: The present invention provides a biofluid sensing device capable of concentrating a biofluid sample with respect to a target analyte, so that the analyte can be accurately detected or measured by EAB sensors. Methods for using such a device provide qualitative information about the presence of the analyte, and/or quantitative information about relative concentrations of the analyte in the biofluid. The disclosed device includes a concentration channel for concentrating the biofluid sample, as well as a selectively permeable membrane, one or more EAB sensors, and one or more secondary sensors carried on a water-impermeable substrate. A method for using the disclosed device to collect a biofluid sample, concentrate the sample relative to a target analyte, and measure the target analyte is also disclosed.

公开(公告)号：US20200093417A1

公开(公告)日：2020-03-26

申请号：US16673037

申请日：2019-11-04

Applicant: Eccrine Systems, Inc. ,  University of Cincinnati

Inventor： Jason Heikenfeld ,  Jacob A. Bertrand ,  Michael Brothers ,  Andrew Jajack

IPC: A61B5/00 ,  G01N33/50 ,  A61B10/00 ,  A61B5/145 ,  A61B5/1468 ,  A61F13/02 ,  G01N33/66 ,  G01N33/53 ,  G01N33/543

Abstract: The disclosed invention provides a sensing device capable of concentrating a fluid sample with respect to a target analyte and a reference analyte, and measuring the target analyte and reference analyte in the concentrated sample. The invention includes a concentrator that may include a selectively permeable membrane, or a gel having an increasing density gradient. In another embodiment, the invention includes an air or gas gap between a fluid transport channel and the concentrator. In another embodiment, the sensing device includes a driver for electronically moving a fluid sample through the channel and membrane to cause concentration. In some embodiments, the invention may also determine the change in molarity of the fluid sample with respect to the target analyte, as the sample is concentrated by the device.

公开(公告)号：US20200033332A1

公开(公告)日：2020-01-30

申请号：US16469358

申请日：2017-12-13

Applicant: Eccrine Systems, Inc.

Inventor： Jacob A. Bertrand ,  Brian Hanley ,  Leila Safazadeh Haghighi ,  Michael Brothers

IPC: G01N33/543 ,  G01N33/53

Abstract: The present invention provides self-assembled monolayers (SAM) configured for use with electrochemical aptamer-based biosensors, which allow sensing devices to detect very low concentrations of target analytes in a biofluid sample. Embodiments of the disclosed invention include SAMs with improved long-term stability in sweat through persistent thiolate bonds between the sensor electrode and disclosed blocker groups, or between the sensor electrode and aptamer sensing elements via disclosed binder molecules. Embodiments of the invention also include blocker groups configured to form densely packed and persistent SAMs on sensor electrodes.

公开(公告)号：US20190117170A1

公开(公告)日：2019-04-25

申请号：US16156328

申请日：2018-10-10

Applicant: Eccrine Systems, Inc.

Inventor： Gavi Begtrup ,  Jacob A. Bertrand ,  Jason Heikenfeld ,  Austin Morgan ,  Nathan Weinle ,  Kathy Cunningham

IPC: A61B5/00 ,  A61B5/145

Abstract: The invention includes sweat sensing devices to measure volumetric sweat rate that use alternating current interrogation of electrodes, and embodiments that have a reusable component and a disposable component. The disclosure further includes a method for detecting an air bubble within a volumetric sweat rate device. The disclosure also includes methods, systems, and non-transitory computer-readable storage medium claims for using a device configured to perform periodic volumetric sweat rate measurements to inform estimates of total body water loss, to aid in the avoidance of heat stress or dehydration, and to facilitate comparison of environmental influences on heat stress or dehydration.

公开(公告)号：US10736565B2

公开(公告)日：2020-08-11

申请号：US15754141

申请日：2017-10-16

Applicant: Eccrine Systems, Inc.

Inventor： Gavi Begtrup ,  Austin Morgan ,  Mikel Larson ,  Jacob A. Bertrand ,  Nicholas Bailey ,  Cory Newland ,  Robert Beech ,  Brian Hanley

IPC: A61B5/00 ,  A61B5/145 ,  A61B5/1477 ,  A61B5/0205 ,  A61B5/20 ,  G01N33/487 ,  A61B5/11 ,  A61B5/024 ,  A61B5/053 ,  A61B5/01

Abstract: Embodiments of the disclosed invention provide devices and methods to incorporate suspension-based, i.e., hydrogel-based and thixotropic compound-based, ion-selective electrodes and reference electrodes into a wearable sweat sensing device. Embodiments of this device are configured to monitor sweat electrolyte concentrations, trends, and ratios under demanding use conditions. The accompanying method includes use of the disclosed device to track fluid and electrolyte gain and loss in order to produce an electrolyte estimate, such as a sweat electrolyte concentration, a sweat electrolyte concentration trend, a sweat rate, or a concentration ratio between a plurality of electrolytes.