公开(公告)号：US12235265B2

公开(公告)日：2025-02-25

申请号：US16308145

申请日：2017-06-08

Applicant: Denka Company Limited

Inventor： Daisuke Kato ,  Tomohiro Hattori ,  Shino Muramatsu

IPC: G01N33/543 ,  G01N1/40 ,  G01N33/569

Abstract: The present invention relates to an immunochromatography test method of measuring a sugar chain antigen, which provides an immunochromatographic test piece and a specimen adding device capable of specifically measuring a sugar chain antigen, and an immunochromatography method using the same; wherein after mixing a specimen with a nitrite solution, a step of allowing tartaric acid to come into contact with the mixture, and extracting a sugar chain antigen contained in the specimen is carried out in a filtration step.

公开(公告)号：US12013394B2

公开(公告)日：2024-06-18

申请号：US16492442

申请日：2018-03-14

Applicant: Denka Company Limited

Inventor： Daisuke Kato ,  Shino Muramatsu ,  Tomohiro Hattori

IPC: G01N33/543

CPC classification number: G01N33/54386

Abstract: The present disclosure provides an immunochromatographic test piece which prevents a non-specific reaction by efficiently and continuously contacting and neutralizing a developing solution containing nitrous acid with a neutralizing reagent in an immunochromatography method of extracting and measuring a sugar chain antigen by nitrous acid extraction on the immunochromatographic test piece. The present disclosure also provides an immunochromatographic test piece for extracting and measuring a sugar chain antigen in a specimen, which comprises: a sample pad; a label region; a detection region on which the antibody against the sugar chain antigen is immobilized; a region impregnated with a neutralizing reagent upstream of the label region, and a region impregnated with a solid acid reagent or a region impregnated with nitrite, upstream of the region impregnated with the neutralizing reagent.

公开(公告)号：US11906513B2

公开(公告)日：2024-02-20

申请号：US16492427

申请日：2018-03-14

Applicant: Denka Company Limited

Inventor： Daisuke Kato ,  Shino Muramatsu ,  Tomohiro Hattori

IPC: G01N33/543 ,  B01L3/00 ,  G01N33/53

CPC classification number: G01N33/54388 ,  B01L3/5023 ,  G01N33/5308 ,  G01N33/543 ,  B01L2200/16 ,  B01L2300/0825

Abstract: It is an object of the present invention to provide a method or an immunochromatographic test piece, which controls a speed or a direction of development of a specimen on the immunochromatographic test piece, so that a treatment with an acid reagent, nitrite, and a neutralizing reagent is properly controlled. The present invention provides an immunochromatographic test piece for extracting and measuring a sugar chain antigen in a specimen, the immunochromatographic test piece having a neutralizing region impregnated with a neutralizing reagent upstream of a label region, and further having a solid acid reagent or nitrite region impregnated with a solid acid reagent, or with nitrite, upstream of the neutralizing region, wherein a resin-made sheet is sandwiched between the solid acid reagent or nitrite region the neutralizing region so as to suppress the movement of a reagent or the movement of a specimen solution between the regions.