公开(公告)号：US10798952B2

公开(公告)日：2020-10-13

申请号：US15435335

申请日：2017-02-17

申请人： DSM IP Assets B.V.

发明人： Kirk E. Apt ,  Paul Warren Behrens ,  Jon Milton Hansen ,  Joseph W. Pfeifer, III ,  Tracey Lynn Stahl ,  Ross Zirkle

IPC分类号： A23D9/00 ,  C11B1/00 ,  C12P7/64 ,  A61K31/202 ,  A23K20/158 ,  A61K8/36 ,  A61K31/201 ,  A61Q19/00 ,  A23L2/52 ,  A23C9/152 ,  A23D9/007 ,  C11B1/10 ,  C11B3/04 ,  C12R1/89 ,  C12R1/90 ,  A23L33/00 ,  A23L33/115 ,  A61K35/68 ,  A23K10/12 ,  A23K50/00 ,  A23K50/40 ,  A23K50/80

摘要： The present invention is directed to isolated microorganisms as well as strains and mutants thereof, biomasses, microbial oils, compositions, and cultures; methods of producing the microbial oils, biomasses, and mutants; and methods of using the isolated microorganisms, biomasses, and microbial oils.

公开(公告)号：US10757959B2

公开(公告)日：2020-09-01

申请号：US16132992

申请日：2018-09-17

申请人： DSM IP Assets B.V.

发明人： Kirk E. Apt ,  Joseph W. Pfeifer, III ,  Jon Milton Hansen ,  Paul Warren Behrens ,  Ross Zirkle ,  Tracey Lynn Stahl

IPC分类号： A61K31/232 ,  A23K20/158 ,  A61K31/20 ,  C12P7/64 ,  C12R1/89 ,  A23L33/115 ,  A23L33/135 ,  A23K50/80 ,  A23D9/00 ,  A23K50/00 ,  A23L33/12 ,  A61K8/99 ,  A61K31/202 ,  A61K36/02 ,  A61Q19/00 ,  A61K35/00

摘要： The present invention is directed to isolated thraustochytrid microorganisms as well as strains and mutants thereof. The invention is further directed to biomasses, microbial oils, compositions, cultures, methods of producing microbial oils, and methods of using the isolated thraustochytrids, biomasses, and microbial oils.

公开(公告)号：US20190000114A1

公开(公告)日：2019-01-03

申请号：US16132992

申请日：2018-09-17

申请人： DSM IP Assets B.V.

发明人： Kirk E. Apt ,  Joseph W. Pfeifer, III ,  Jon Milton Hansen ,  Paul Warren Behrens ,  Ross Zirkle ,  Tracey Lynn Stahl

IPC分类号： A23K20/158 ,  C12R1/89 ,  A23K50/00 ,  C12P7/64 ,  A61Q19/00 ,  A23K50/80 ,  A23L33/12 ,  A61K36/02 ,  A61K31/202 ,  A61K31/20 ,  A61K8/99 ,  A23D9/00 ,  A23L33/135 ,  A23L33/115 ,  A61K35/00

摘要： The present invention is directed to isolated thraustochytrid microorganisms as well as strains and mutants thereof The invention is further directed to biomasses, microbial oils, compositions, cultures, methods of producing microbial oils, and methods of using the isolated thraustochytrids, biomasses, and microbial oils.

公开(公告)号：US09611488B2

公开(公告)日：2017-04-04

申请号：US14698498

申请日：2015-04-28

申请人： DSM IP Assets B.V.

发明人： Kirk E. Apt ,  Paul Warren Behrens ,  Jon Milton Hansen ,  Joseph W. Pfeifer, III ,  Tracey Lynn Stahl ,  Ross Zirkle

IPC分类号： A23D9/00 ,  C12P7/64 ,  A61K31/202

CPC分类号： A61K31/202 ,  A23K20/158 ,  A23K50/80 ,  A23L2/52 ,  A23L33/12 ,  A23L33/40 ,  A23V2002/00 ,  A61K8/361 ,  A61K2800/10 ,  A61Q19/00 ,  C12P7/6427 ,  A61K2300/00

摘要： The invention is directed to microbial oils containing omega-3 polyunsaturated fatty acids comprising docosahexaenoic acid, eicosapentaenoic acid, and optionally docosapentaenoic acid and dosage forms containing such oils.

公开(公告)号：US09518102B2

公开(公告)日：2016-12-13

申请号：US14548054

申请日：2014-11-19

申请人： DSM IP Assets B.V.

发明人： Kirk E. Apt ,  James Casey Lippmeier ,  David Simpson ,  Jun Wang ,  James P. Wynn ,  Ross Zirkle

IPC分类号： C12N15/62 ,  C07K14/705 ,  C07K14/37 ,  C12N15/80 ,  C12P21/00 ,  C12R1/90 ,  C12N15/79

CPC分类号： C12P21/00 ,  C07K14/37 ,  C07K14/705 ,  C12N9/2431 ,  C12N15/79 ,  C12N15/80 ,  C12P21/005 ,  C12R1/90 ,  C12Y302/01026

摘要： The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

摘要翻译： 本发明涉及神经胶霉病菌门的重组细胞和微生物及其在异源蛋白质生产中的用途。 用于有效生产和任选地从重组宿主细胞和微生物分泌多肽的新型启动子，终止子和信号序列也包括在本发明中。

公开(公告)号：US09012616B2

公开(公告)日：2015-04-21

申请号：US14108794

申请日：2013-12-17

申请人： DSM IP Assets B.V.

发明人： Kirk E. Apt ,  James Casey Lippmeier ,  David Simpson ,  Jun Wang ,  James P. Wynn ,  Ross Zirkle

IPC分类号： C07H21/04 ,  C12N1/13 ,  C12P21/02 ,  C07K14/37 ,  C12N15/80 ,  C12P21/00 ,  C12R1/90

CPC分类号： C12P21/00 ,  C07K14/37 ,  C07K14/705 ,  C12N9/2431 ,  C12N15/79 ,  C12N15/80 ,  C12P21/005 ,  C12R1/90 ,  C12Y302/01026

摘要： The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

摘要翻译： 本发明涉及神经胶霉病菌门的重组细胞和微生物及其在异源蛋白质生产中的用途。 用于有效生产和任选地从重组宿主细胞和微生物分泌多肽的新型启动子，终止子和信号序列也包括在本发明中。

公开(公告)号：US08859855B2

公开(公告)日：2014-10-14

申请号：US13633770

申请日：2012-10-02

申请人： DSM IP Assets B.V.

发明人： Craig A. Weaver ,  Ross Zirkle ,  Daniel H. Doherty ,  James G. Metz

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N1/20 ,  C07H21/04 ,  C12P7/64 ,  C12P7/62 ,  C12N9/00 ,  C12N15/52

CPC分类号： C12P7/6427 ,  C12N9/93 ,  C12N15/52 ,  C12N15/8247 ,  C12N15/8273 ,  C12P7/62 ,  C12P7/625 ,  C12P7/6472

摘要： Disclosed are chimeric polyunsaturated fatty acid (PUFA) olyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from Schizochytrium and Thraustochytrium. Disclosed are nucleic acids and proteins encoding such chimeric PUFA PKS proteins and systems, genetically modified organisms comprising such chimeric PUFA PKS proteins and systems, and methods of making and using such chimeric PUFA PKS proteins and systems.

摘要翻译： 公开了嵌合多不饱和脂肪酸（PUFA）合成酮合成酶（PKS）蛋白和嵌合PUFA PKS系统，包括嵌合PUFA PKS蛋白和源自裂殖壶菌和破囊壶菌的系统。 公开了编码这种嵌合PUFA PKS蛋白质和系统的核酸和蛋白质，包含这种嵌合PUFA PKS蛋白质和系统的遗传修饰的生物体，以及制备和使用这种嵌合PUFA PKS蛋白质和系统的方法。

公开(公告)号：US20210395765A1

公开(公告)日：2021-12-23

申请号：US17354558

申请日：2021-06-22

申请人： Dow AgroSciences LLC ,  DSM IP Assets B.V.

发明人： Terence A. Walsh ,  Daniel J. Gachotte ,  Cory M. Larsen ,  Scott Bevan ,  P. Ann Owens-Merlo ,  James G. Metz ,  Ross Zirkle

IPC分类号： C12N15/82

摘要： This disclosure concerns recombinant host organisms genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.

公开(公告)号：US20170164639A1

公开(公告)日：2017-06-15

申请号：US15435335

申请日：2017-02-17

申请人： DSM IP Assets B.V.

发明人： Kirk E. APT ,  Paul Warren Behrens ,  Jon Milton Hansen ,  Joseph W. Pfeifer, III ,  Tracey Lynn Stahl ,  Ross Zirkle

IPC分类号： A23K20/158 ,  A23K50/80 ,  A23K50/40 ,  C11B1/10 ,  A23K50/00

摘要： The present invention is directed to isolated microorganisms as well as strains and mutants thereof, biomasses, microbial oils, compositions, and cultures; methods of producing the microbial oils, biomasses, and mutants; and methods of using the isolated microorganisms, biomasses, and microbial oils.

公开(公告)号：US11154076B2

公开(公告)日：2021-10-26

申请号：US15889842

申请日：2018-02-06

申请人： DSM IP Assets B.V.

发明人： Kirk E. Apt ,  Paul Warren Behrens ,  Jon Milton Hansen ,  Joseph W. Pfeifer, III ,  Tracey Lynn Stahl ,  Ross Zirkle

IPC分类号： A23D9/00 ,  C12P7/64 ,  A61K31/202 ,  A23K20/158 ,  A61K8/36 ,  A61K31/201 ,  A61Q19/00 ,  A23L2/52 ,  A23C9/152 ,  A23D9/007 ,  C11B1/10 ,  C11B3/04 ,  A23L33/00 ,  A23L33/115 ,  A61K35/68 ,  A23K10/12 ,  A23K50/00 ,  A23K50/40 ,  A23K50/80 ,  C12N1/10 ,  C12N1/12 ,  C12R1/89 ,  C12R1/90

摘要： The present invention is directed to isolated microorganisms as well as strains and mutants thereof, biomasses, microbial oils, compositions, and cultures; methods of producing the microbial oils, biomasses, and mutants; and methods of using the isolated microorganisms, biomasses, and microbial oils.