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公开(公告)号：US09163232B2
公开(公告)日：2015-10-20
申请号：US12339490
申请日：2008-12-19
CPC分类号： C12N15/1093 , C07K16/18 , C07K16/2833 , C07K16/36 , C07K16/40 , C07K2317/55 , C40B50/06
摘要： The invention relates to a method for manufacturing a recombinant polyclonal protein composition, in particular a recombinant polyclonal antibody composition. The method comprises obtaining a collection of cells transfected with a library of variant nucleic acid sequences, wherein each call in the collection is transfected with and capable of expressing one member of the library, which encodes a distinct member of a polyclonal protein that binds a particular antigen and which is located at the same single site in the genome of individual cells in said collection, wherein said nucleic acid sequence is not naturally associated with said cell in the collection. The cells are cultured under suitable conditions for expression of the polyclonal protein, which is obtained from the cells or culture supernatant. The present method is suitable for manufacturing recombinant polyclonal antibodies, thereby making available a superior replacement of plasma-derived therapeutic immunoglobulin products.
摘要翻译： 本发明涉及制备重组多克隆蛋白质组合物，特别是重组多克隆抗体组合物的方法。 该方法包括获得用变体核酸序列文库转染的细胞的集合，其中集合中的每个调用被转染并且能够表达文库的一个成员，其编码结合特定的多克隆蛋白的不同成员 抗原，其位于所述收集物中个体细胞的基因组中的相同单个位点，其中所述核酸序列不与所述细胞中的所述细胞天然相关。 细胞在合适的条件下培养，用于表达从细胞或培养物上清液获得的多克隆蛋白质。 本发明方法适用于制备重组多克隆抗体，从而提供了血浆衍生的治疗性免疫球蛋白产品的优异替代品。
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公开(公告)号：US20090136498A1
公开(公告)日：2009-05-28
申请号：US12339490
申请日：2008-12-19
IPC分类号： A61K39/395 , C40B30/06 , C40B30/04 , C12P21/04 , C07K14/00 , A61K38/17 , C40B40/02 , C40B40/08 , C07K16/18
CPC分类号： C12N15/1093 , C07K16/18 , C07K16/2833 , C07K16/36 , C07K16/40 , C07K2317/55 , C40B50/06
摘要： The invention relates to a method for manufacturing a recombinant polyclonal protein composition, in particular a recombinant polyclonal antibody composition. The method comprises obtaining a collection of cells transfected with a library of variant nucleic acid sequences, wherein each call in the collection is transfected with and capable of expressing one member of the library, which encodes a distinct member of a polyclonal protein that binds a particular antigen and which is located at the same single site in the genome of individual cells in said collection, wherein said nucleic acid sequence is not naturally associated with said cell in the collection. The cells are cultured under suitable conditions for expression of the polyclonal protein, which is obtained from the cells or culture supernatant. The nucleic acid sequence id introduced into the cells by transfection with a library of vectors for site-specific integration. The present method is suitable for manufacturing recombinant polyclonal antibodies, thereby making available a superior replacement of plasma-derived therapeutic immunoglobulin products.
摘要翻译： 本发明涉及制备重组多克隆蛋白质组合物，特别是重组多克隆抗体组合物的方法。 该方法包括获得用变体核酸序列文库转染的细胞的集合，其中集合中的每个调用被转染并且能够表达文库的一个成员，其编码结合特定的多克隆蛋白的不同成员 抗原，其位于所述收集物中个体细胞的基因组中的相同单个位点，其中所述核酸序列不与所述细胞中的所述细胞天然相关。 细胞在合适的条件下培养，用于表达从细胞或培养物上清液获得的多克隆蛋白质。 通过用载体文库转染用于位点特异性整合的核酸序列ID被引入细胞。 本发明方法适用于制备重组多克隆抗体，从而提供了血浆衍生的治疗性免疫球蛋白产品的优异替代品。
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公开(公告)号：US20080118535A1
公开(公告)日：2008-05-22
申请号：US11177423
申请日：2005-07-11
申请人： Chiou-Ying Yang
发明人： Chiou-Ying Yang
IPC分类号： A61K39/095 , A61P31/04 , C07H21/04
摘要： Present invention provides a monoclonal antibody binding to Neisseria bacteria and its target antigen Ag473, which include the sequences of its polynucleotide and its amino acid, wherein the Neisseria bacteria can be Neisseria meningitidis or Neisseria gonorrhoeae; and wherein Ag473 can be made into a vaccine or a diagnostic or therapeutic reagent.
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公开(公告)号：US07357932B2
公开(公告)日：2008-04-15
申请号：US10867662
申请日：2004-06-16
申请人： Chiou-Ying Yang
发明人： Chiou-Ying Yang
IPC分类号： A61K39/02 , A61K38/00 , A61K39/095
摘要： Present invention provides a monoclonal antibody binding to Neisseria bacteria and its target antigen Ag473, which include the sequences of its polynucleotide and its amino acid, wherein the Neisseria bacteria can be Neisseria meningitidis or Neisseria gonorrhoeae; and wherein Ag473 can be made into a vaccine or a diagnostic or therapeutic reagent.
摘要翻译： 本发明提供了结合奈瑟氏球菌及其靶抗原Ag473的单克隆抗体，其包括其多核苷酸及其氨基酸的序列，其中奈瑟氏菌属细菌可以是脑膜炎奈瑟氏球菌或淋病奈瑟氏球菌; 并且其中Ag473可以制成疫苗或诊断或治疗试剂。
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公开(公告)号：US20060275766A1
公开(公告)日：2006-12-07
申请号：US10540227
申请日：2004-01-07
申请人： John Haurum , Finn Wiberg , Vincent Coljee , Jacqueline Sharon , Chiou-Ying Yang
发明人： John Haurum , Finn Wiberg , Vincent Coljee , Jacqueline Sharon , Chiou-Ying Yang
CPC分类号： C12N15/1082 , C07K16/00 , C07K16/18 , C07K16/2833 , C07K16/36 , C07K16/40 , C07K2317/55
摘要： The invention relates to a method for manufacturing a recombinant polyclonal protein composition, in particular a recombinant polyclonal antibody composition. The method comprises obtaining a collection of cells transfected with a library of variant nucleic acid sequences, wherein each cell in the collection is transfected with and capable of expressing one member of the library, which encodes a distinct member of a polyclonal protein that binds a particular antigen and which is located at the same single site in the genome of individual cells in said collection, wherein said nucleic acid sequence is not naturally associated with said cell in the collection. The cells are cultured under suitable conditions for expression of the polyclonal protein, which is obtained from the cells or culture supernatant. The nucleic acid sequence id introduced into the cells by transfection with a library of vectors for site-specific integration. The present method is suitable for manufacturing recombinant polyclonal antibodies, thereby mailing available a superior replacement of plasma-derived therapeutic immunoglobulin products.
摘要翻译： 本发明涉及制备重组多克隆蛋白质组合物，特别是重组多克隆抗体组合物的方法。 该方法包括获得用变体核酸序列文库转染的细胞的集合，其中集合中的每个细胞被转染并且能够表达文库的一个成员，其编码结合特异性的多克隆蛋白质的不同成员 抗原，其位于所述收集物中个体细胞的基因组中的相同单个位点，其中所述核酸序列不与所述细胞中的所述细胞天然相关。 细胞在合适的条件下培养，用于表达从细胞或培养物上清液获得的多克隆蛋白质。 通过用载体文库转染用于位点特异性整合的核酸序列ID被引入细胞。 本发明方法适用于制备重组多克隆抗体，从而邮寄可获得的等离子体衍生治疗性免疫球蛋白产品的优异替代物。
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公开(公告)号：US20050281835A1
公开(公告)日：2005-12-22
申请号：US10867662
申请日：2004-06-16
申请人： Chiou-Ying Yang
发明人： Chiou-Ying Yang
摘要： Present invention provides a monoclonal antibody binding to Neisseria bacteria and its target antigen Ag473, which include the sequences of its polynucleotide and its amino acid, wherein the Neisseria bacteria can be Neisseria meningitidis or Neisseria gonorrhoeae; and wherein Ag473 can be made into a vaccine or a diagnostic or therapeutic reagent.
摘要翻译： 本发明提供了结合奈瑟氏球菌及其靶抗原Ag473的单克隆抗体，其包括其多核苷酸及其氨基酸的序列，其中奈瑟氏菌属细菌可以是脑膜炎奈瑟氏球菌或淋病奈瑟氏球菌; 并且其中Ag473可以制成疫苗或诊断或治疗试剂。
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