-
公开(公告)号：US20240018184A1
公开(公告)日：2024-01-18
申请号：US18354413
申请日：2023-07-18
IPC分类号： C07K1/22 , B01J20/26 , B01J20/285 , B01J20/286 , B01J20/32 , C07K14/31 , C07K16/00 , C07K16/12 , C07K17/10
CPC分类号： C07K1/22 , B01J20/267 , B01J20/285 , B01J20/286 , B01J2220/52 , C07K14/31 , C07K16/00 , C07K16/1271 , C07K17/10 , B01J20/3274
摘要： The present invention concerns a method of cleaning and/or sanitizing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of:
a) optionally purifying a mixture comprising a first immunoglobulin using the separation matrix;
b) providing a cleaning liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; and
c) cleaning and/or sanitizing the separation matrix by contacting the cleaning liquid with the separation matrix for a predetermined contact time.
The alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO 51 or SEQ ID NO 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.-
公开(公告)号：US11708390B2
公开(公告)日：2023-07-25
申请号：US16095721
申请日：2017-05-10
IPC分类号： C07K1/22 , B01D15/00 , C07K14/31 , B01J20/286 , B01J20/32 , C07K16/06 , C07K16/12 , C07K17/10
CPC分类号： C07K1/22 , B01D15/00 , B01J20/286 , B01J20/3212 , B01J20/3274 , C07K14/31 , C07K16/065 , C07K16/1271 , C07K17/10 , B01J2220/52
摘要： The present invention concerns a method of storing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) providing a storage liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; b) permeating the separation matrix with the storage liquid; and c) storing the storage liquid-permeated separation matrix for a storage time of at least days. The alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by, or having at least 80% such as at least 90%, 95% or 98% identity to, SEQ ID NO 51 or SEQ ID NO 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
-
公开(公告)号：US20220315647A1
公开(公告)日：2022-10-06
申请号：US17604964
申请日：2020-04-29
发明人： Gustav Rodrigo , Mats Ander , Tomas Bjorkman , Ronnie Palmgren , Charlotte Brink
摘要： The invention discloses a method for separation of antibodies or antibody fragments, comprising the steps of: a) providing a feed comprising antibodies or antibody fragments having a VH3 region and being devoid of an Fc region capable of binding to Protein A; b) contacting the feed with a separation resin having covalently coupled ligands, wherein the ligands comprise a polypeptide as defined by SEQ ID NO 1 and wherein the antibodies or antibody fragments bind to the separation resin; c) optionally washing the separation resin with a washing liquid; d) eluting the antibodies or antibody fragments from the separation resin with an elution liquid and recovering the antibodies or antibody fragments.
-
公开(公告)号：US20210163529A1
公开(公告)日：2021-06-03
申请号：US16955119
申请日：2018-12-13
发明人： Gustav Rodrigo , Mats Ander , Ronnie Palmgren , Tomas Bjorkman
IPC分类号： C07K1/22 , B01J20/24 , B01J20/28 , B01J20/288 , B01J20/32 , B01J20/30 , C07K16/00 , B01D15/38
摘要： A method for preparation of a separation matrix, comprising the steps of: a) providing a solid support and an alkali-stable ligand derived from an immunoglobulin-binding bacterial protein; b) reacting said alkali-stable ligand with said solid support to form a separation matrix having covalently coupled alkali-stable ligands; and c) washing said separation matrix having covalently coupled alkali-stable ligands with a wash solution comprising at least 10 mM of an alkali metal hydroxide.
-
公开(公告)号：US12037359B2
公开(公告)日：2024-07-16
申请号：US17114773
申请日：2020-12-08
发明人： Gustav José Rodrigo , Tomas Bjorkman , Mats Ander
IPC分类号： C07K1/22 , B01D15/38 , B01J20/24 , B01J20/26 , B01J20/28 , B01J20/285 , B01J20/286 , B01J20/32 , C07K14/31 , C07K16/00 , C07K16/06 , C07K16/12 , C07K17/10
CPC分类号： C07K1/22 , B01D15/3809 , B01J20/24 , B01J20/267 , B01J20/28016 , B01J20/28078 , B01J20/285 , B01J20/286 , B01J20/3212 , B01J20/3274 , C07K14/31 , C07K16/00 , C07K16/065 , C07K16/1271 , C07K17/10 , B01J2220/52
摘要： An Fc-binding polypeptide of improved alkali stability, comprising a mutant of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 22, SEQ ID NO: 51 or SEQ ID NO: 52, wherein at least the asparagine or serine residue at the position corresponding to position 11 in SEQ ID NO: 4-7 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
-
公开(公告)号：US11136359B2
公开(公告)日：2021-10-05
申请号：US15900419
申请日：2018-02-20
发明人： Gustav Rodrigo , Mats Ander , Tomas Bjorkman , Goran Bauren
IPC分类号： C07K14/31 , B01D15/38 , B01J20/286 , B01J20/32 , C07K1/22 , C07K14/745 , C07K16/00
摘要： The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 9 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.
-
公开(公告)号：US10995113B2
公开(公告)日：2021-05-04
申请号：US16893574
申请日：2020-06-05
IPC分类号： A23J1/00 , C07K1/22 , B01D15/38 , B01J20/24 , B01J20/28 , B01J20/286 , B01J20/32 , C07K14/31 , C07K16/00 , C07K16/06 , C07K17/10
摘要： The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
-
公开(公告)号：US11753438B2
公开(公告)日：2023-09-12
申请号：US16095753
申请日：2017-05-10
IPC分类号： C07K1/22 , B01J20/26 , B01J20/285 , B01J20/286 , B01J20/32 , C07K14/31 , C07K16/00 , C07K16/12 , C07K17/10
CPC分类号： C07K1/22 , B01J20/267 , B01J20/285 , B01J20/286 , B01J20/3274 , C07K14/31 , C07K16/00 , C07K16/1271 , C07K17/10 , B01J2220/52
摘要： The present invention concerns a method of cleaning and/or sanitizing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) optionally purifying a mixture comprising a first immunoglobulin using the separation matrix; b) providing a cleaning liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; and c) cleaning and/or sanitizing the separation matrix by contacting the cleaning liquid with the separation matrix for a predetermined contact time. The alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO 51 or SEQ ID NO 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
-
公开(公告)号：US20230279047A1
公开(公告)日：2023-09-07
申请号：US18298857
申请日：2023-04-11
IPC分类号： C07K1/22 , B01D15/38 , B01J20/24 , B01J20/28 , B01J20/286 , B01J20/32 , C07K14/31 , C07K16/00 , C07K16/06 , C07K17/10
CPC分类号： C07K1/22 , B01D15/3809 , B01J20/24 , B01J20/28016 , B01J20/28078 , B01J20/286 , B01J20/3212 , B01J20/3274 , C07K14/31 , C07K16/00 , C07K16/065 , C07K17/10
摘要： The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein:
a) the ligands comprise multimers of alkali-stabilized Protein A domains, and
b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.-
公开(公告)号：US11566082B2
公开(公告)日：2023-01-31
申请号：US15525688
申请日：2015-11-11
发明人： Gustav Rodrigo , Tomas Bjorkman , Mats Ander
IPC分类号： C07K16/46 , B01D15/38 , B01J20/289 , B01J20/32 , C07K1/22 , C07K14/31 , C07K16/00 , C07K16/06
摘要： An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, wherein at least the alanine residue at the position corresponding to position 42 in SEQ ID NO:4-7 has been mutated to arginine and/or wherein at least the aspartic acid residue at the position corresponding to position 37 in SEQ ID NO:4-7 has been mutated to glutamic acid.
-
-
-
-
-
-
-
-
-
搜索结果
25 条记录 (耗时 0.021 秒)
