公开(公告)号：US20110207171A1

公开(公告)日：2011-08-25

申请号：US13007506

申请日：2011-01-14

Applicant: Brian Agnew ,  Schuyler B. CORRY ,  Kyle R. GEE

Inventor： Brian Agnew ,  Schuyler B. CORRY ,  Kyle R. GEE

IPC: C12P33/00 ,  C12P21/00 ,  C07K16/00 ,  C12N9/96

CPC classification number: C12P21/005 ,  A61K47/549 ,  A61K47/61 ,  C07K1/13 ,  C07K14/47 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/41 ,  C12Y302/01096 ,  G01N33/5005 ,  G01N33/5008 ,  G01N33/531 ,  G01N33/532 ,  G01N33/533 ,  G01N33/534 ,  G01N33/581 ,  G01N33/582 ,  G01N33/583 ,  Y10T436/17

Abstract: The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.

Abstract translation: 本发明通常涉及在寡糖键上官能化蛋白质，特别是抗体的方法，通过修饰糖基化来人源化抗体的方法，以及与修饰的寡糖连接的新型抗体。 本发明还涉及可用于产生本发明抗体的试剂盒。

公开(公告)号：US20110159517A1

公开(公告)日：2011-06-30

申请号：US13030743

申请日：2011-02-18

Applicant: Kyle R. GEE

Inventor： Kyle R. GEE

IPC: G01N33/53 ,  C07D311/90 ,  C07D413/12 ,  C08B37/00 ,  G01N33/20

CPC classification number: C07D311/82 ,  C08B37/0039 ,  G01N31/22 ,  G01N33/5308 ,  G01N33/6872

Abstract: The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of zinc ions in a sample. The metal chelating moiety of the zinc-binding compound is an analog of the well-known calcium chelator, BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid), wherein the chelating moiety has been modified from a tetraacetic acid moiety to a tri- di- or monoacetic moiety. This change in acetic acid groups on the metal chelating moiety results in the selective bindings of zinc ions in the presence of calcium ions, both of which are present in biological fluids and intracellular cytosolic fluid and organelles.

Abstract translation: 本发明提供金属螯合剂和促进样品中锌离子结合，检测，监测和定量的方法。 锌结合化合物的金属螯合部分是众所周知的钙螯合剂BAPTA（1,2-双（2-氨基苯氧基）乙烷-N，N，N'，N'-四乙酸）的类似物，其中 螯合部分已从四乙酸部分修饰为三 - 或一乙酸部分。 金属螯合部分上乙酸基团的这种变化导致锌离子在钙离子存在下的选择性结合，两者都存在于生物流体和细胞内胞质溶液和细胞器中。