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公开(公告)号：US10732088B2
公开(公告)日：2020-08-04
申请号：US16030174
申请日：2018-07-09
摘要： Disclosed is a sheath delivery system that uses a continuous flow of sheath fluid into a pressurized internal reservoir that substantially matches the outflow of sheath fluid through the nozzle of a flow cytometer. A substantially constant level of the sheath fluid is maintained. If the sheath fluid level falls below a desired level, or goes above a desired level, a dampened control system is used to reach the desired level. In addition, air pressure in the pressurized internal container is controlled so that an external sheath container can be removed and refilled with additional sheath fluid without stopping the sheath delivery system 100. Differences in pressure are detected by a droplet camera, which measures the droplet breakoff point to determine the pressure of the sheath fluid in the nozzle.
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公开(公告)号：US20170051735A1
公开(公告)日：2017-02-23
申请号：US15242122
申请日：2016-08-19
CPC分类号： F04B43/1261 , F04B43/0072 , F04B43/1215 , F04B43/1276
摘要： A method and apparatus for pumping fluid through tubing are provided. The method includes orbiting first rollers around the periphery of a first disk at a first tangential speed in a first angular sector and a slower, second tangential speed in a second angular sector, orbiting second rollers around the periphery of a second disk at the second tangential speed, and increasing the pressure of fluid in tubing between one first roller and one second roller by causing the one first roller to fully compress the tubing at the first tangential speed and simultaneously causing the one second roller to fully compress the tubing at the second tangential speed. The apparatus includes a first disk with a recess in its periphery, the first angular sector with a nominal first radius, and a second angular sector with a nominal second radius; and a second disk with the nominal first radius and a recess in its periphery.
摘要翻译： 提供了一种通过管道泵送流体的方法和装置。 该方法包括以第一角扇区中的第一切向速度围绕第一圆盘的圆周绕行第一滚轮，并且在第二角扇区中以较慢的第二切向速度围绕第一圆盘的绕第二滚轮旋转第二滚轮 通过使一个第一辊以第一切向速度完全压缩管道并同时使一个第二辊在第二切向下完全压缩管道，从而增加一个第一辊和一个第二辊之间的管道中的流体的压力 速度。 该装置包括在其周边具有凹部的第一盘，具有标称第一半径的第一角扇区和具有标称第二半径的第二角扇区; 以及具有标称第一半径的第二盘和在其周边的凹部。
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公开(公告)号：US20170160186A1
公开(公告)日：2017-06-08
申请号：US15376504
申请日：2016-12-12
IPC分类号： G01N15/14
CPC分类号： G01N15/1404 , G01N1/28 , G01N1/286 , G01N2015/0065 , G01N2015/1006 , G01N2015/1406 , G01N2015/1409 , Y10T137/0324 , Y10T137/7287 , Y10T137/7303 , Y10T137/85978
摘要： Disclosed is a sheath delivery system that uses a continuous flow of sheath fluid into a pressurized internal reservoir that substantially matches the outflow of sheath fluid through the nozzle of a flow cytometer. A substantially constant level of the sheath fluid is maintained. If the sheath fluid level falls below a desired level, or goes above a desired level, a dampened control system is used to reach the desired level. In addition, air pressure in the pressurized internal container is controlled so that an external sheath container can be removed and refilled with additional sheath fluid without stopping the sheath delivery system 100. Differences in pressure are detected by a droplet camera, which measures the droplet breakoff point to determine the pressure of the sheath fluid in the nozzle.
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4.发明授权Flow rate balanced, dynamically adjustable sheath delivery system for flow cytometry 有权流量平衡，动态可调护套输送系统，用于流式细胞术公开(公告)号：US09551637B2
公开(公告)日：2017-01-24
申请号：US13918156
申请日：2013-06-14
CPC分类号： G01N15/1404 , G01N1/28 , G01N1/286 , G01N2015/0065 , G01N2015/1006 , G01N2015/1406 , G01N2015/1409 , Y10T137/0324 , Y10T137/7287 , Y10T137/7303 , Y10T137/85978
摘要： Disclosed is a sheath delivery system that uses a continuous flow of sheath fluid into a pressurized internal reservoir that substantially matches the outflow of sheath fluid through the nozzle of a flow cytometer. A substantially constant level of the sheath fluid is maintained. If the sheath fluid level falls below a desired level, or goes above a desired level, a dampened control system is used to reach the desired level. In addition, air pressure in the pressurized internal container is controlled so that an external sheath container can be removed and refilled with additional sheath fluid without stopping the sheath delivery system 100. Differences in pressure are detected by a droplet camera, which measures the droplet breakoff point to determine the pressure of the sheath fluid in the nozzle.
摘要翻译： 公开了一种护套递送系统，其使用鞘流体连续流入加压的内部储存器，其基本上匹配鞘液通过流式细胞仪的喷嘴的流出。 保持基本恒定水平的鞘液。 如果护套液体液位低于期望水平或高于所需水平，则使用阻尼控制系统达到所需水平。 此外，加压的内部容器中的空气压力被控制，使得可以移除外部护套容器并且用另外的护套流体重新填充，而不停止护套输送系统100.压力的差异通过液滴照相机来检测，其测量液滴断裂 以确定喷嘴中鞘液的压力。
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公开(公告)号：US08980200B2
公开(公告)日：2015-03-17
申请号：US13923622
申请日：2013-06-21
CPC分类号： B01L3/56 , G01N15/1404 , G01N2015/1409 , G01N2015/1413 , Y10T29/49826
摘要： Disclosed is a nozzle assembly that is compact in size and that uses plastic tubing as an injection needle. Standard plastic fittings are utilized, which are inexpensive and widely available. The nozzle assembly has a simple construction and can be easily assembled and disassembled in a few minutes by a user. Cleaning and/or replacement of parts is inexpensive. Plastic tubing can be used as an injection needle that has superior qualities over commonly used stainless steel injection needles. Flexure of the injection needle tubing is prevented because of the compact size of the nozzle cavity.
摘要翻译： 公开了一种尺寸紧凑并且使用塑料管作为注射针的喷嘴组件。 使用标准的塑料配件，其便宜且广泛可用。 喷嘴组件具有简单的结构，并且可以在几分钟内由用户容易地组装和拆卸。 清洁和/或更换零件便宜。 塑料管可用作注射针，其具有比常用的不锈钢注射针优异的质量。 由于喷嘴腔的紧凑尺寸，防止注射针管弯曲。
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公开(公告)号：US20130340539A1
公开(公告)日：2013-12-26
申请号：US13923480
申请日：2013-06-21
IPC分类号： B01L9/00
CPC分类号： B01L9/52 , B01L9/06 , G01N15/14 , G01N35/1011 , G01N2015/149
摘要： Disclosed is a collection sled for collecting a waste droplet stream 122 in a waste stream catcher that is located below sample collectors. Contamination of collected samples is reduced by collecting the waste droplet stream at a position that is below the sample collectors. A waste trough is provided that gets progressively larger in a downward direction, which prevents backsplash of waste fluid from the collection cavity. Standard sample collector tubes, as well as 8-well strips and microscope slides can be used with the collection sled to collect sample particles.
摘要翻译： 公开了一种用于在位于样品收集器下方的废物流捕集器中收集废液滴流122的收集筒。 通过在低于样品收集器的位置处收集废液滴流来减少收集的样品的污染。 提供了在向下方向逐渐变大的废物槽，其防止废液从收集腔中脱落。 标准样品收集器管以及8孔条和显微镜载玻片可与收集雪橇一起使用以收集样品颗粒。
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公开(公告)号：US10309388B2
公开(公告)日：2019-06-04
申请号：US15242122
申请日：2016-08-19
摘要： A method and apparatus for pumping fluid through tubing are provided. The method includes orbiting first rollers around the periphery of a first disk at a first tangential speed in a first angular sector and a slower, second tangential speed in a second angular sector, orbiting second rollers around the periphery of a second disk at the second tangential speed, and increasing the pressure of fluid in tubing between one first roller and one second roller by causing the one first roller to fully compress the tubing at the first tangential speed and simultaneously causing the one second roller to fully compress the tubing at the second tangential speed. The apparatus includes a first disk with a recess in its periphery, the first angular sector with a nominal first radius, and a second angular sector with a nominal second radius; and a second disk with the nominal first radius and a recess in its periphery.
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公开(公告)号：US10036697B2
公开(公告)日：2018-07-31
申请号：US15376504
申请日：2016-12-12
CPC分类号： G01N15/1404 , G01N1/28 , G01N1/286 , G01N2015/0065 , G01N2015/1006 , G01N2015/1406 , G01N2015/1409 , Y10T137/0324 , Y10T137/7287 , Y10T137/7303 , Y10T137/85978
摘要： Disclosed is a sheath delivery system that uses a continuous flow of sheath fluid into a pressurized internal reservoir that substantially matches the outflow of sheath fluid through the nozzle of a flow cytometer. A substantially constant level of the sheath fluid is maintained. If the sheath fluid level falls below a desired level, or goes above a desired level, a dampened control system is used to reach the desired level. In addition, air pressure in the pressurized internal container is controlled so that an external sheath container can be removed and refilled with additional sheath fluid without stopping the sheath delivery system 100. Differences in pressure are detected by a droplet camera, which measures the droplet breakoff point to determine the pressure of the sheath fluid in the nozzle.
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公开(公告)号：US09791362B2
公开(公告)日：2017-10-17
申请号：US15417131
申请日：2017-01-26
IPC分类号： G01N15/14
CPC分类号： G01N15/14 , B01F3/088 , B01F13/0059 , B01F15/0441 , G01N15/1404 , G01N2015/1006 , G01N2015/1409 , G05D11/131
摘要： Disclosed is a system that can mix deionized water and concentrated sheath fluid to provide sheath fluid in a flow cytometer system having a desired concentration. Flow rates are low, which substantially match the flow rate of sheath fluid through the nozzle, so that turbulence and air bubbles are not formed in the sheath fluid. The available deionized water is then used for back flushing and removal of sample cells and deposited salts from the sheath fluid.
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公开(公告)号：US09592483B2
公开(公告)日：2017-03-14
申请号：US13922635
申请日：2013-06-20
CPC分类号： G01N15/14 , B01F3/088 , B01F13/0059 , B01F15/0441 , G01N15/1404 , G01N2015/1006 , G01N2015/1409 , G05D11/131
摘要： Disclosed is a system that can mix deionized water and concentrated sheath fluid to provide sheath fluid in a flow cytometer system having a desired concentration. Flow rates are low, which substantially match the flow rate of sheath fluid through the nozzle, so that turbulence and air bubbles are not formed in the sheath fluid. The available deionized water is then used for back flushing and removal of sample cells and deposited salts from the sheath fluid.
摘要翻译： 公开了一种可以将去离子水和浓缩鞘液混合以在具有所需浓度的流式细胞仪系统中提供鞘液的系统。 流速低，这与通过喷嘴的鞘液的流速基本一致，从而在鞘液中不形成湍流和气泡。 然后可用的去离子水用于从皮肤液中进行反冲洗和去除样品池和沉积的盐。
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