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公开(公告)号：US20170276686A1
公开(公告)日：2017-09-28
申请号：US15510962
申请日：2015-09-15
发明人： Edward Marcotte , Eric V. Anslyn , Andrew Ellington , Jagannath Swaminathan , Erik Hernandez , Amber Johnson , Alexander Boulgakov , James L. Bachman , Helen Seifert
摘要： The present invention relates to the field of identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level. The present invention also relates to methods for identifying amino acids in peptides, including peptides comprising unnatural amino acids. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeling an internal amino acid with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.
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公开(公告)号：US11105812B2
公开(公告)日：2021-08-31
申请号：US15461034
申请日：2017-03-16
IPC分类号： G01N33/68
摘要： The present invention relates to methods for identifying amino acids in peptides. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeling an internal amino acid with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.
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公开(公告)号：US20140120574A1
公开(公告)日：2014-05-01
申请号：US13909345
申请日：2013-06-04
发明人： Eric V. Anslyn , Youjun Yang , Michelle A. Ivy
CPC分类号： G01N33/84 , A61K31/455 , C07D215/12 , G01N31/22 , Y10T436/177692
摘要： Nitric oxide probes comprising a compound represented by Formula I are provided. Methods of using this nitric oxide probes to detect nitric oxide are also provided.
摘要翻译： 提供了包含由式I表示的化合物的一氧化氮探针。 还提供了使用这种一氧化氮探针来检测一氧化氮的方法。
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公开(公告)号：US08637323B2
公开(公告)日：2014-01-28
申请号：US13645217
申请日：2012-10-04
发明人： Eric V. Anslyn , Youjun Yang , Michelle M. Adams
CPC分类号： G01N31/224 , C07C255/58 , C07D471/06
摘要： Nitric oxide probes including a compound represented by Formula, I, II, III, IV, V, VI or a combination thereof are provided. Methods of using these nitric oxide probes to detect nitric oxide are also provided.
摘要翻译： 提供了包含由式I，II，III，IV，V，VI表示的化合物或其组合的一氧化氮探针。 还提供了使用这些一氧化氮探针来检测一氧化氮的方法。
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公开(公告)号：US20240124910A1
公开(公告)日：2024-04-18
申请号：US18264025
申请日：2022-02-02
摘要： Disclosed are methods, systems, components, and compositions for synthesis of sequence defined polymers. The methods, systems, components, and compositions may be utilized for incorporating novel substrates that include non-standard amino acid monomers and non-amino acid monomers into sequence defined polymers. As disclosed herein, the novel substrates may be utilized for acylation of tRNA via flexizyme catalyzed reactions. The tRNAs thus acylated with the novel substrates may be utilized in synthesis platforms for incorporating the novel substrates into a sequence defined polymer.
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公开(公告)号：US11435358B2
公开(公告)日：2022-09-06
申请号：US17491797
申请日：2021-10-01
摘要： Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides with unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.
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公开(公告)号：US11162952B2
公开(公告)日：2021-11-02
申请号：US16572194
申请日：2019-09-16
发明人： Edward Marcotte , Eric V. Anslyn , Andrew Ellington , Jagannath Swaminathan , Erik Hernandez , Amber Johnson , Alexander Boulgakov , James L. Bachman , Helen Seifert
摘要： The disclosure concerns methods for identifying amino acids in peptides, including peptides including unnatural amino acids. Various aspects of the present disclosure provide compositions and methods for amino acid-type specific labeling, as well as methods for detecting the labels. Further disclosed herein are strategies for highly multiplexed, high-throughput peptide analysis.
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公开(公告)号：US10545153B2
公开(公告)日：2020-01-28
申请号：US15510962
申请日：2015-09-15
发明人： Edward Marcotte , Eric V. Anslyn , Andrew Ellington , Jagannath Swaminathan , Erik Hernandez , Amber Johnson , Alexander Boulgakov , James L. Bachman , Helen Seifert
摘要： Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides with one or more unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.
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公开(公告)号：US20140179014A1
公开(公告)日：2014-06-26
申请号：US14135918
申请日：2013-12-20
发明人： Eric V. Anslyn , Youjun Yang , Michelle M. Adams
IPC分类号： G01N31/22
CPC分类号： G01N31/224 , C07C255/58 , C07D471/06
摘要： Nitric oxide probes including a compound represented by Formula, I, II, III, IV, V, VI or a combination thereof are provided. Methods of using these nitric oxide probes to detect nitric oxide are also provided.
摘要翻译： 提供了包含由式I，II，III，IV，V，VI表示的化合物或其组合的一氧化氮探针。 还提供了使用这些一氧化氮探针来检测一氧化氮的方法。
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公开(公告)号：US20130302903A1
公开(公告)日：2013-11-14
申请号：US13645217
申请日：2012-10-04
发明人： Eric V. Anslyn , Youjun Yang , Michelle M. Adams
IPC分类号： C07C255/58 , C07C211/54 , C07C309/46 , C07C229/64 , C07D455/06 , C07C217/80 , C07C217/94 , G01N21/64 , C07C229/56
CPC分类号： G01N31/224 , C07C255/58 , C07D471/06
摘要： Nitric oxide probes comprising a compound represented by Formula, I, II, III, IV, V, VI or a combination thereof are provided. Methods of using these nitric oxide probes to detect nitric oxide are also provided.
摘要翻译： 提供了包含由式I，II，III，IV，V，VI表示的化合物或其组合的一氧化氮探针。 还提供了使用这些一氧化氮探针来检测一氧化氮的方法。
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