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    Protein that enhances expression of potassium channels on cell surfaces and nucleic acids that encode the same
    4.
    发明授权
    Protein that enhances expression of potassium channels on cell surfaces and nucleic acids that encode the same 失效
    增强细胞表面上钾通道表达的蛋白质和编码其的核酸

    公开(公告)号：US06207422B1

    公开(公告)日：2001-03-27

    申请号：US09062440

    申请日：1998-04-17

    申请人： Arthur M. Brown ,  Barbara A. Wible ,  Qing Yang

    发明人： Arthur M. Brown ,  Barbara A. Wible ,  Qing Yang

    IPC分类号： C12P1934

    CPC分类号： C07K14/705 ,  C07K14/47 ,  C12Q1/6876

    摘要： The present invention provides polynucleotides that encode a protein, designated herein as K+ Channel Associated Protein or “KChAP”. It has been determined that expressing polynucleotides that encode KChAP in host cells, along with polynucleotides that encode the Kv&agr; channel subunit Kv 2.1, the Kv&agr; channel subunit Kv 2.2, the Kv&agr; channel subunit Kv 1.3, or the Kv&agr; channel subunit Kv 4.3, increases the number of Kv2.1, Kv 2.2, Kv1.3 or Kv4.3 channels, respectively, in the plasma membrane of such cells. The present invention also relates to a method of making cells that have increased numbers of Kv channels on the plasma membranes thereof and to a method of using such cells as model systems for studying the effect of pharmacological agents on Kv channels, particularly on Kv2.1, Kv 2.2, Kv 1.3, and Kv 4.3 channels. The present invention also relates to the protein KChAP.

    摘要翻译： 本发明提供编码本文称为K +通道相关蛋白或“KChAP”的蛋白质的多核苷酸。 已经确定，表达编码宿主细胞中的KChAP的多核苷酸以及编码Kvalpha通道亚基Kv2.1，Kvalpha通道亚基Kv 2.2，Kvalpha通道亚基Kv 1.3或Kvalpha通道亚基Kv 4.3的多核苷酸增加了 Kv2.1，Kv 2.2，Kv1.3或Kv4.3通道的数量分别在这些细胞的质膜中。 本发明还涉及一种制备在其质膜上具有增加的Kv通道数目的细胞的方法，以及使用这种细胞作为模型系统的方法，用于研究药理剂对Kv通道的影响，特别是Kv2.1 ，Kv 2.2，Kv 1.3和Kv 4.3通道。 本发明还涉及蛋白质KChAP。

    Methods of inducing apoptosis in hyperproliferative cells
    5.
    发明授权
    Methods of inducing apoptosis in hyperproliferative cells 失效
    诱导过度增殖细胞凋亡的方法

    公开(公告)号：US08187586B2

    公开(公告)日：2012-05-29

    申请号：US10784528

    申请日：2004-02-23

    申请人： Arthur M. Brown ,  Barbara A. Wible

    发明人： Arthur M. Brown ,  Barbara A. Wible

    IPC分类号： A01N63/00 ,  A01N65/00 ,  A61K48/00 ,  C12N15/00

    CPC分类号： C07K14/4702 ,  A61K38/00

    摘要： Methods of inducing apoptosis in hyperproliferative cells, particularly cancer cells are provided. Such method involves increasing the levels of a potassium channel modulatory protein in the cell. Examples of such proteins are native KChAP protein, a biologically active variant of native KChAP protein, or a biologically active KChAP-related protein (collectively referred to hereinafter as “KChAP protein”). In one embodiment, the cells are contacted with the KChAP protein under conditions permitting uptake of the protein by the cells. In another embodiment, the cells are contacted with (i) a nucleic acid encoding the KChAP protein, and (ii) a promoter active in the cancer cell, wherein the promoter is operably linked to the region encoding the KChAP protein, under conditions permitting the uptake of the nucleic acid by the cancer cell. Methods of detecting cancerous cells in a biological sample selected from the group consisting of a colorectal tissue sample or brain tissue sample are also provided. Such method comprises assaying for the presence of elevated levels of KChAP mRNA or KChAP protein in the sample.

    摘要翻译： 提供了诱导过度增殖细胞，特别是癌细胞凋亡的方法。 这种方法包括增加细胞中钾通道调节蛋白的水平。 这种蛋白质的实例是天然KChAP蛋白，天然KChAP蛋白的生物活性变体或生物活性KChAP相关蛋白（以下统称为“KChAP蛋白”）。 在一个实施方案中，细胞在允许细胞摄取蛋白质的条件下与KChAP蛋白质接触。 在另一个实施方案中，细胞与（i）编码KChAP蛋白的核酸和（ii）在癌细胞中有活性的启动子接触，其中所述启动子在条件允许的情况下可操作地连接到编码KChAP蛋白的区域 癌细胞摄取核酸。 还提供了检测选自结肠直肠组织样品或脑组织样品的生物样品中的癌细胞的方法。 该方法包括测定样品中KChAP mRNA或KChAP蛋白水平的升高。

    Protein that enhances expression of potassium channels on cell surfaces and nucleic acids that encode the same
    6.
    发明授权
    Protein that enhances expression of potassium channels on cell surfaces and nucleic acids that encode the same 有权
    增强细胞表面上钾通道表达的蛋白质和编码其的核酸

    公开(公告)号：US06391561B1

    公开(公告)日：2002-05-21

    申请号：US09712495

    申请日：2000-11-14

    申请人： Arthur M. Brown ,  Barbara A. Wible ,  Qing Yang

    发明人： Arthur M. Brown ,  Barbara A. Wible ,  Qing Yang

    IPC分类号： C12Q168

    CPC分类号： C07K14/705 ,  C07K14/47 ,  C12Q1/6876

    摘要： The present invention provides polynucleotides that encode a protein, designated herein as K+ Channel Associated Protein or “KChAP”. It has been determined that expressing polynucleotides that encode KChAP in host cells, along with polynucleotides that encode the Kv&agr; channel subunit Kv 2.1, the Kv&agr; channel subunit Kv 2.2, the Kv&agr; channel subunit Kv 1.3, or the Kv&agr; channel subunit Kv 4.3, increases the number of Kv2.1, Kv 2.2, Kv1.3 or Kv4.3 channels, respectively, in the plasma membrane of such cells. The present invention also relates to a method of making cells that have increased numbers of Kv channels on the plasma membranes thereof and to a method of using such cells as model systems for studying the effect of pharmacological agents on Kv channels, particularly on Kv2.1, Kv 2.2, Kv 1.3, and Kv 4.3 channels. The present invention also relates to the protein KChAP.

    摘要翻译： 本发明提供编码本文称为K +通道相关蛋白或“KChAP”的蛋白质的多核苷酸。 已经确定表达编码宿主细胞中的KChAP的多核苷酸以及编码Kvalpha通道亚基Kv2.1，Kvalpha通道亚基Kv 2.2，Kvalpha通道亚基Kv 1.3或Kvalpha通道亚基Kv 4.3的多核苷酸增加了 Kv2.1，Kv 2.2，Kv1.3或Kv4.3通道的数量分别在这些细胞的质膜中。 本发明还涉及一种制备在其质膜上具有增加的Kv通道数目的细胞的方法，以及使用这种细胞作为模型系统的方法，用于研究药理剂对Kv通道的影响，特别是Kv2.1 ，Kv 2.2，Kv 1.3和Kv 4.3通道。 本发明还涉及蛋白质KChAP。