Protein that enhances expression of potassium channels on cell surfaces and nucleic acids that encode the same

发明授权

US06207422B1 Protein that enhances expression of potassium channels on cell surfaces and nucleic acids that encode the same 失效

标题翻译：增强细胞表面上钾通道表达的蛋白质和编码其的核酸

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专利标题： Protein that enhances expression of potassium channels on cell surfaces and nucleic acids that encode the same
专利标题（中）： 增强细胞表面上钾通道表达的蛋白质和编码其的核酸
申请号： US09062440

申请日： 1998-04-17
公开(公告)号： US06207422B1

公开(公告)日： 2001-03-27
发明人: Arthur M. Brown , Barbara A. Wible , Qing Yang
申请人： Arthur M. Brown , Barbara A. Wible , Qing Yang
主分类号： C12P1934
IPC分类号： C12P1934

Protein that enhances expression of potassium channels on cell surfaces and nucleic acids that encode the same

摘要：

The present invention provides polynucleotides that encode a protein, designated herein as K+ Channel Associated Protein or “KChAP”. It has been determined that expressing polynucleotides that encode KChAP in host cells, along with polynucleotides that encode the Kv&agr; channel subunit Kv 2.1, the Kv&agr; channel subunit Kv 2.2, the Kv&agr; channel subunit Kv 1.3, or the Kv&agr; channel subunit Kv 4.3, increases the number of Kv2.1, Kv 2.2, Kv1.3 or Kv4.3 channels, respectively, in the plasma membrane of such cells. The present invention also relates to a method of making cells that have increased numbers of Kv channels on the plasma membranes thereof and to a method of using such cells as model systems for studying the effect of pharmacological agents on Kv channels, particularly on Kv2.1, Kv 2.2, Kv 1.3, and Kv 4.3 channels. The present invention also relates to the protein KChAP.

摘要（中）：

本发明提供编码本文称为K +通道相关蛋白或“KChAP”的蛋白质的多核苷酸。已经确定，表达编码宿主细胞中的KChAP的多核苷酸以及编码Kvalpha通道亚基Kv2.1，Kvalpha通道亚基Kv 2.2，Kvalpha通道亚基Kv 1.3或Kvalpha通道亚基Kv 4.3的多核苷酸增加了 Kv2.1，Kv 2.2，Kv1.3或Kv4.3通道的数量分别在这些细胞的质膜中。本发明还涉及一种制备在其质膜上具有增加的Kv通道数目的细胞的方法，以及使用这种细胞作为模型系统的方法，用于研究药理剂对Kv通道的影响，特别是Kv2.1 ，Kv 2.2，Kv 1.3和Kv 4.3通道。本发明还涉及蛋白质KChAP。

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