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公开(公告)号：US5210028A
公开(公告)日：1993-05-11
申请号：US616470
申请日：1990-11-21
申请人： Albert Schmitz , Walter Marki
发明人： Albert Schmitz , Walter Marki
IPC分类号： C12N15/02 , A61K38/00 , A61K38/27 , C07K1/113 , C07K1/14 , C07K1/20 , C07K14/575 , C07K14/62 , C07K14/65 , C12N1/21 , C12N15/09 , C12N15/16 , C12N15/17 , C12N15/73 , C12P21/02 , C12R1/19
摘要： A process for the preparation of a recombinant IGF-II (rIGF-II) without a covalently attached foreign protein moiety and without N-terminal attached methionine or a derivative of methionine or of a salt of said IGF-II, rIGF-II produced by said method, hybrid vectors comprising DNA encoding said rIGF-II, hosts transformed with said vectors, and a process for the isolation of said rIGF-II from the host cell and refolding it into a biologically active form.
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2.发明授权Process for the production and refolding of unfused IGF-II protein in E. coli 失效在大肠杆菌中生产和重折叠未融合的IGF-II蛋白的方法
公开(公告)号：US5708148A
公开(公告)日：1998-01-13
申请号：US341275
申请日：1994-11-16
申请人： Albert Schmitz , Walter Marki
发明人： Albert Schmitz , Walter Marki
IPC分类号： C12N15/02 , A61K38/00 , A61K38/27 , C07K1/113 , C07K1/14 , C07K1/20 , C07K14/575 , C07K14/62 , C07K14/65 , C12N1/21 , C12N15/09 , C12N15/16 , C12N15/17 , C12N15/73 , C12P21/02 , C12R1/19 , C12N15/18
摘要： A process for the preparation of a recombinant IGF-II (rIGF-II) without a covalently attached foreign protein moiety and without N-terminal attached methionine or a derivative of methionine or of a salt of said IGF-II, rIGF-II produced by said method, hybrid vectors comprising DNA encoding said rIGF-II, hosts transformed with said vectors, and a process for the isolation of said rIGF-II from the host cell and refolding it into a biologically active form.
摘要翻译： 制备不具有共价连接的外来蛋白质部分并且不具有N-末端连接的甲硫氨酸或甲硫氨酸衍生物或所述IGF-II，rIGF-II的盐的重组IGF-II（rIGF-II）的制备方法，所述方法由 所述方法，包含编码所述rIGF-II的DNA的混合载体，用所述载体转化的宿主，以及从宿主细胞中分离所述rIGF-II并将其重折叠成生物活性形式的方法。
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公开(公告)号：US5422249A
公开(公告)日：1995-06-06
申请号：US121974
申请日：1993-09-15
申请人： Manfred Liersch , Hans Rink , Walter Marki , Markus G. Grutter , Bernd Meyhack
发明人： Manfred Liersch , Hans Rink , Walter Marki , Markus G. Grutter , Bernd Meyhack
IPC分类号： C12N15/09 , A61K35/62 , A61K35/74 , A61K38/55 , A61P7/02 , C07H21/00 , C07H21/04 , C07K1/20 , C07K14/00 , C07K14/815 , C07K16/00 , C07K16/38 , C12N1/00 , C12N5/00 , C12N5/10 , C12N9/99 , C12N15/00 , C12N15/10 , C12N15/15 , C12P21/00 , C12P21/02 , C12R1/125 , C12R1/19 , C12R1/865 , C12N15/70
CPC分类号： C07H21/00 , C07K14/815 , C07K16/38 , C12N15/10
摘要： The invention relates to DNA sequences that code for the amino acid sequence of the thrombin inhibitor hirudin, hybrid vectors containing such DNA sequences, host cells transformed with such hybrid vectors, novel polypeptides with thrombin-inhibiting activity produced from such transformed host cells, processes for the manufacture of these DNA sequences, hybrid vectors and transformed host cells, and processes for the manufacture of these thrombin-inhibitors using the transformed host cells. The hirudin compounds that can be produced according to the invention have valuable pharmacological properties.
摘要翻译： 本发明涉及编码凝血酶抑制剂水蛭素的氨基酸序列的DNA序列，含有这些DNA序列的杂交载体，用这种杂交载体转化的宿主细胞，由这种转化的宿主细胞产生的具有凝血酶抑制活性的新多肽， 这些DNA序列的制造，杂合载体和转化的宿主细胞，以及使用转化的宿主细胞制备这些凝血酶抑制剂的方法。 根据本发明可以生产的水蛭素化合物具有有价值的药理学性质。
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公开(公告)号：US5661001A
公开(公告)日：1997-08-26
申请号：US406948
申请日：1995-03-29
申请人： Hugo Grossenbacher , Jui Yoa Chang , Walter Marki
发明人： Hugo Grossenbacher , Jui Yoa Chang , Walter Marki
IPC分类号： C12N15/09 , A61K38/00 , A61K38/55 , A61K38/58 , A61P7/02 , C07K1/10 , C07K1/113 , C07K14/815 , C12N1/19 , C12N15/15 , C12N15/81 , C12P21/02 , C12R1/865 , A61K38/03 , C12P21/06
CPC分类号： C07K14/815 , A61K38/00
摘要： The invention pertains to the field of thrombin inhibitors and describes the production of modified desulphatohirudins, particularly desulphatohirudins, with the aid of genetic engineering. The present invention is also directed to a method for the denaturation and renaturation of a modified desulphatohirudin to a biologically active thrombin inhibitor and the combination of two or more of desulphatohirudins to form higher molecular weight thrombin inhibitors.
摘要翻译： PCT No.PCT / EP94 / 02438 Sec。 371日期1995年3月29日 102（e）1995年3月29日PCT PCT 1994年7月23日PCT公布。 出版物WO95 / 04823 日期1995年2月16日本发明涉及凝血酶抑制剂领域，并描述了在遗传工程的帮助下生产改性脱硫水蛭素，特别是脱硫水蛭素。 本发明还涉及将改性脱硫水蛭素变性和复性为生物活性凝血酶抑制剂和两种或多种脱硫水蛭素的组合以形成较高分子量凝血酶抑制剂的方法。
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公开(公告)号：US5728549A
公开(公告)日：1998-03-17
申请号：US398416
申请日：1995-03-03
IPC分类号： C12N15/09 , A61K35/62 , A61K35/74 , A61K38/55 , A61P7/02 , C07H21/00 , C07H21/04 , C07K1/20 , C07K14/00 , C07K14/815 , C07K16/00 , C07K16/38 , C12N1/00 , C12N5/00 , C12N5/10 , C12N9/99 , C12N15/00 , C12N15/10 , C12N15/15 , C12P21/00 , C12P21/02 , C12R1/125 , C12R1/19 , C12R1/865 , C12N15/75 , C12N15/81
CPC分类号： C07H21/00 , C07K14/815 , C07K16/38 , C12N15/10
摘要： The invention relates to DNA sequences that code for the amino acid sequence of the thrombin inhibitor hirudin, hybrid vectors containing such DNA sequences, host cells transformed with such hybrid vectors, novel polypeptides with thrombin-inhibiting activity produced from such transformed host cells, processes for the manufacture of these DNA sequences, hybrid vectors and transformed host cells, and processes for the manufacture of these thrombin-inhibitors using the transformed host cells. The hirudin compounds that can be produced according to the invention have valuable pharmacological properties.
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