Endophytes and related methods
    5.
    发明授权

    公开(公告)号:US10881060B2

    公开(公告)日:2021-01-05

    申请号:US14692094

    申请日:2015-04-21

    Abstract: The present invention relates to a method for identifying and/or characterising an endophyte strain, said method including providing a plurality of samples of endophytes, subjecting said endophytes to genetic analysis; subjecting said endophytes to metabolic analysis and selecting endophytes having a desired genetic and metabolic profile. The present invention also relates to novel endophytes having a desired toxin profile wherein the endophyte produces significantly less toxic alkaloids compared with a control endophyte such as standard toxic (ST) endophyte; and/or significantly more alkaloids conferring beneficial properties compared with a control endophyte such as ST endophyte. The present invention also relates to endophyte variants having a desired genetic and metabolic profile, wherein said endophyte variants possess genetic and/or metabolic characteristics that result in a beneficial phenotype in a plant harbouring or otherwise associated with the endophyte variant. Preferably said endophyte variants are generated by polyploidisation or induced chromosome doubling.

    Selection of Symbiota by Screening Multiple Host-Symbiont Associations
    6.
    发明申请
    Selection of Symbiota by Screening Multiple Host-Symbiont Associations 审中-公开
    通过筛选多个主机 - 协议协议来选择Symbiota

    公开(公告)号:US20150156982A1

    公开(公告)日:2015-06-11

    申请号:US14404861

    申请日:2013-05-29

    CPC classification number: A01H17/00 A01H5/10 C12N15/1058 C12Q1/68

    Abstract: The present invention relates to new methods of selecting and breeding organisms, in particular organisms which exhibit symbiotic behaviour with symbionts, and to new organisms and symbiota developed thereby, such as plant or grass and endophyte symbiota. Multiple symbionts are deployed in multiple organisms and selected for improved symbiotic compatibility and performance early in the breeding process. Methods include producing improved organisms from germplasm, by inoculating host organism germplasm libraries with symbionts selected from symbiont libraries and selecting improved host organisms exhibiting desired symbiota characteristics, and selection of organism-symbiont associations with a desired genetic and metabolic profile by metagenomic analysis of nucleic acid libraries from an organism or organism-symbiont association.

    Abstract translation: 本发明涉及选择和繁殖生物体的新方法,特别是具有共生体共生行为的生物,以及由此开发的新生物和共生菌,例如植物或草和内生真菌共生体。 在多种生物体中部署多种共生体,并在育种过程的早期选择提高共生相容性和表现。 方法包括从种质生产改良的生物,通过接种具有选自共生体文库的共生体的宿主生物种质文库,并选择具有所需共生特征的改良宿主生物,以及通过核酸的宏基因组分析选择具有所需遗传和代谢特征的生物 - 共生体缔合 来自生物体或生物共生体结合的文库。

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