公开(公告)号：US10808015B2

公开(公告)日：2020-10-20

申请号：US15738555

申请日：2016-06-24

Applicant: AMYRIS, INC. ,  TOTAL MARKETING SERVICES

Inventor： Penelope R. Chua ,  Hanxiao Jiang ,  Adam Leon Meadows

IPC: C07K14/395 ,  C12N1/16 ,  C12P19/62 ,  C12P5/02 ,  C12P7/64 ,  C12P21/00 ,  C12N15/52 ,  C12N15/63 ,  C12N15/65 ,  C12P21/02

Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.

公开(公告)号：US10563229B2

公开(公告)日：2020-02-18

申请号：US14910240

申请日：2013-08-07

Applicant: AMYRIS, INC. ,  TOTAL MARKETING SERVICES

Inventor： Penelope R. Chua ,  Adam Meadows

IPC: C12P7/00 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04 ,  C07H21/02 ,  C12P5/00 ,  C12N15/81 ,  C12N15/63

Abstract: The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.

公开(公告)号：US11230716B2

公开(公告)日：2022-01-25

申请号：US16733191

申请日：2020-01-02

Applicant: Amyris, Inc. ,  Total Marketing Services

Inventor： Penelope R. Chua ,  Adam Meadows

IPC: C12N1/20 ,  C12N9/00 ,  C12N15/00 ,  C12N15/81 ,  C12N15/63 ,  C12N1/16 ,  C12P5/00

Abstract: The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.

公开(公告)号：US10988513B2

公开(公告)日：2021-04-27

申请号：US15738918

申请日：2016-06-24

Applicant: AMYRIS, INC. ,  TOTAL MARKETING SERVICES

Inventor： Penelope R. Chua ,  Hanxiao Jiang ,  Adam Leon Meadows

IPC: C07K14/395 ,  C12N1/16 ,  C12P19/62 ,  C12P5/02 ,  C12P7/64 ,  C12P21/00 ,  C12N15/52 ,  C12N15/63 ,  C12N15/65 ,  C12P21/02

Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.

公开(公告)号：US12098407B2

公开(公告)日：2024-09-24

申请号：US17616843

申请日：2020-06-05

Applicant: AMYRIS, INC.

Inventor： Penelope R. Chua ,  Joshua Adam Lerman ,  Thomas Jon Scherbart ,  Chandresh Thakker ,  Annie Ening Tsong ,  Hanxiao Jiang

IPC: C12N1/14 ,  C12N1/18 ,  C12N9/02 ,  C12N9/14 ,  C12N15/81 ,  C12P5/00

CPC classification number: C12P5/007 ,  C12N1/18 ,  C12N9/0036 ,  C12N9/14 ,  C12N15/81

Abstract: Provided herein are compositions and methods for uncoupling the yield and productivity of an isoprenoid compound produced in a host cell. In some embodiments, the yield and productivity are uncoupled by genetically modifying the host cell to reduce flux through the citric acid cycle (TCA). In other embodiments, the yield and productivity are uncoupled by reducing the levels of ATP in the host cell.