公开(公告)号：US10858676B2

公开(公告)日：2020-12-08

申请号：US15982623

申请日：2018-05-17

申请人： AJINOMOTO CO., INC.

发明人： Christine Roche ,  Benjamin Mijts ,  Peter Kelly ,  Matthew Davis ,  Sayaka Asari ,  Keita Fukui ,  Miku Toyazaki

IPC分类号： C12P7/42 ,  C12P13/12 ,  C12R1/15 ,  C12R1/19 ,  C12N15/77 ,  C12N9/78 ,  C12N9/88 ,  C12N9/10 ,  C12P7/22 ,  C12P7/24

摘要： A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so as to have a specific feature, such as a reduced activity of AICAR formyltransferase/IMP cyclohydrolase, an increased activity of 3-PGDH, and/or a reduced activity of L-serine deaminase.

公开(公告)号：US09487806B2

公开(公告)日：2016-11-08

申请号：US14735303

申请日：2015-06-10

申请人： AJINOMOTO CO., INC.

发明人： Miku Toyazaki ,  Keiko Noguchi ,  Mika Moriya ,  Yuri Uehara

IPC分类号： C12P13/04 ,  C12P13/08 ,  C12N15/70

CPC分类号： C12P13/08 ,  C12N15/70 ,  C12P13/04

摘要： A method for producing of an L-amino acid is provided. An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability, which has been modified so that the acpP-fabF operon is attenuated, in a medium, and collecting the L-amino acid from the medium or cells of the bacterium.

摘要翻译： 提供了L-氨基酸的制造方法。 通过培养在培养基中培养属于肠杆菌科并具有L-氨基酸生产能力的细菌，其被修饰以使acpP-fabF操纵子减弱，产生L-氨基酸， 来自培养基的氨基酸或细菌的细胞。

公开(公告)号：US20190249206A1

公开(公告)日：2019-08-15

申请号：US16392791

申请日：2019-04-24

申请人： AJINOMOTO CO., INC.

发明人： Benjamin Mijts ,  Christine Roche ,  Sayaka Asari ,  Miku Toyazaki ,  Keita Fukui

IPC分类号： C12P7/24 ,  C12R1/15 ,  C12R1/19 ,  C12N1/36

CPC分类号： C12P7/24 ,  C12N1/36 ,  C12N2330/50 ,  C12N2510/02 ,  C12P7/42 ,  C12P13/12 ,  C12R1/15 ,  C12R1/19 ,  C12Y101/01001 ,  C12Y101/01025 ,  C12Y113/11003 ,  C12Y114/13082

摘要： A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so that the activity of enolase is reduced.

公开(公告)号：US20180230441A1

公开(公告)日：2018-08-16

申请号：US15951496

申请日：2018-04-12

申请人： AJINOMOTO CO., INC.

发明人： Keita Fukui ,  Keiko Danjo ,  Junko Ito ,  Miku Toyazaki

IPC分类号： C12N9/04 ,  C12P7/24 ,  C12Q1/04

CPC分类号： C12N9/0006 ,  C12P7/24 ,  C12Q1/04 ,  C12Y101/01001

摘要： A method is provided for producing an objective substance, for example, an aromatic aldehyde such as vanillin. The objective substance is produced from a carbon source or a precursor of the objective substance by using a coryneform bacterium that is able to produce the objective substance, wherein bacterium has been modified so that the activity of alcohol dehydrogenase is reduced.

公开(公告)号：US20150275246A1

公开(公告)日：2015-10-01

申请号：US14735303

申请日：2015-06-10

申请人： AJINOMOTO CO., INC.

发明人： Miku Toyazaki ,  Keiko Noguchi ,  Mika Moriya ,  Yuri Uehara

IPC分类号： C12P13/08

CPC分类号： C12P13/08 ,  C12N15/70 ,  C12P13/04

摘要： A method for producing of an L-amino acid is provided. An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability, which has been modified so that the acpP-fabF operon is attenuated, in a medium, and collecting the L-amino acid from the medium or cells of the bacterium.

摘要翻译： 提供了L-氨基酸的制造方法。 通过培养在培养基中培养属于肠杆菌科并具有L-氨基酸生产能力的细菌，其被修饰以使acpP-fabF操纵子减弱，产生L-氨基酸， 来自培养基的氨基酸或细菌的细胞。

公开(公告)号：US20190249205A1

公开(公告)日：2019-08-15

申请号：US16392756

申请日：2019-04-24

申请人： AJINOMOTO CO., INC.

发明人： Benjamin Mijts ,  Christine Roche ,  Peter Kelly ,  Sayaka Asari ,  Keita Fukui ,  Miku Toyazaki

IPC分类号： C12P7/24 ,  C12N1/20 ,  C12N9/10 ,  C12N9/88 ,  C12N9/02 ,  C12N9/04

CPC分类号： C12P7/24 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0069 ,  C12N9/0073 ,  C12N9/1007 ,  C12N9/1085 ,  C12N9/88 ,  C12P7/22 ,  C12P7/42 ,  C12Y101/01001 ,  C12Y101/01025 ,  C12Y102/99007 ,  C12Y113/11003 ,  C12Y114/13082 ,  C12Y201/01015 ,  C12Y205/01054 ,  C12Y402/0101 ,  C12Y402/01118 ,  C12Y402/03004

摘要： A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so that the activity of an L-cysteine biosynthesis enzyme is increased.

公开(公告)号：US20190241914A1

公开(公告)日：2019-08-08

申请号：US16392776

申请日：2019-04-24

申请人： AJINOMOTO CO., INC.

发明人： Benjamin Mijts ,  Christine Roche ,  Peter Kelly ,  Sayaka Asari ,  Miku Toyazaki ,  Keita Fukui

IPC分类号： C12P7/42

CPC分类号： C12P7/42 ,  C12P7/22 ,  C12P7/24 ,  C12P13/001 ,  C12P13/12 ,  C12P17/10 ,  C12P19/40

摘要： A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so that the activity of NCgl2048 protein is reduced.

公开(公告)号：US20180334693A1

公开(公告)日：2018-11-22

申请号：US15982623

申请日：2018-05-17

申请人： AJINOMOTO CO., INC.

发明人： Christine Roche ,  Benjamin Mijts ,  Peter Kelly ,  Matthew Davis ,  Sayaka Asari ,  Keita Fukui ,  Miku Toyazaki

IPC分类号： C12P7/42 ,  C12P13/12 ,  C12R1/15 ,  C12R1/19 ,  C12N15/77 ,  C12N9/10 ,  C12N9/78 ,  C12N9/88

CPC分类号： C12P7/42 ,  C12N9/1003 ,  C12N9/78 ,  C12N9/88 ,  C12N15/77 ,  C12P7/22 ,  C12P7/24 ,  C12P13/12 ,  C12R1/15 ,  C12R1/19 ,  C12Y201/02003 ,  C12Y305/0401 ,  C12Y403/01017

摘要： A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so as to have a specific feature, such as a reduced activity of AICAR formyltransferase/IMP cyclohydrolase, an increased activity of 3-PGDH, and/or a reduced activity of L-serine deaminase.

公开(公告)号：US11680279B2

公开(公告)日：2023-06-20

申请号：US16200011

申请日：2018-11-26

申请人： AJINOMOTO CO., INC.

发明人： Benjamin Mijts ,  Christine Roche ,  Rekha Murali ,  Sayaka Asari ,  Keita Fukui ,  Miku Toyazaki ,  Keiko Noguchi

IPC分类号： C12P13/12 ,  C12P7/24 ,  C12N9/10 ,  C12N9/06 ,  C12P7/42 ,  C12N1/20 ,  C12R1/15 ,  C12R1/19 ,  C12R1/46

CPC分类号： C12P7/24 ,  C12N1/205 ,  C12N9/0012 ,  C12N9/1014 ,  C12N9/1085 ,  C12P7/42 ,  C12P13/12 ,  C12R2001/15 ,  C12R2001/19 ,  C12R2001/46 ,  C12Y105/0102 ,  C12Y201/02002 ,  C12Y205/01006

摘要： A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism that is able to produce the objective substance, which microorganism has been modified so that the activity of an enzyme involved in SAM cycle (SAM cycle enzyme) is increased.

公开(公告)号：US11053519B2

公开(公告)日：2021-07-06

申请号：US16392805

申请日：2019-04-24

申请人： AJINOMOTO CO., INC.

发明人： Benjamin Mijts ,  Christine Roche ,  Peter Kelly ,  Sayaka Asari ,  Keita Fukui ,  Miku Toyazaki

IPC分类号： C12P7/42 ,  C12P7/24 ,  C12N9/14 ,  C12P13/12 ,  A23L27/20 ,  A23L27/24

摘要： A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so that the activity of S-adenosyl-L-homocysteine hydrolase is increased.