公开(公告)号：US11511016B2

公开(公告)日：2022-11-29

申请号：US16363471

申请日：2019-03-25

申请人： INSERM (Institut National de la Santé et de la Recherche Médicale) ,  UNIVERSITE PARIS 7—DENIS DIDEROT

发明人： Catherine Le Visage ,  Didier Letourneur

IPC分类号： A61L27/20 ,  A61L27/38 ,  A61L27/50 ,  A61L27/52 ,  A61L27/56 ,  C12N5/00 ,  C08B37/00 ,  C08B37/02 ,  C08B37/08 ,  C08J9/08 ,  C08J9/28 ,  A61L27/54

摘要： The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprises the steps consisting of: a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide, an amount of a cross-linking agent and an amount of a porogen agent b) transforming the solution into a hydrogel by placing said solution at a temperature from about 4° C. to about 80° C. for a sufficient time to allow the cross-linking of said amount of polysaccharide and c) submerging said hydrogel into an aqueous solution d) washing the porous scaffold obtained at step c).

公开(公告)号：US20150246163A1

公开(公告)日：2015-09-03

申请号：US14708996

申请日：2015-05-11

申请人： INSERM (Institut National de la Sante et de la Recherche Medicale) ,  Universite Paris 7 - Denis Diderot

发明人： Catherine Le Visage ,  Didier Letourneur ,  Frederic Chaubet ,  Aude Autissier

IPC分类号： A61L27/56 ,  C08J3/24 ,  A61L27/54 ,  A61L27/20 ,  A61L27/38 ,  A61L27/50 ,  C12N11/10 ,  C08B37/02

CPC分类号： A61L27/56 ,  A61L27/20 ,  A61L27/3804 ,  A61L27/3826 ,  A61L27/3834 ,  A61L27/3847 ,  A61L27/3852 ,  A61L27/507 ,  A61L27/52 ,  A61L27/54 ,  A61L2300/21 ,  A61L2430/02 ,  A61L2430/06 ,  C08B37/0018 ,  C08B37/0021 ,  C08B37/003 ,  C08B37/0039 ,  C08B37/0054 ,  C08B37/0063 ,  C08B37/0072 ,  C08B37/0075 ,  C08B37/0084 ,  C08H1/06 ,  C08J3/24 ,  C08J9/28 ,  C08J2201/026 ,  C08J2201/0484 ,  C08J2205/04 ,  C08J2207/10 ,  C08J2305/00 ,  C08J2305/02 ,  C08J2305/08 ,  C08J2305/12 ,  C12N5/0068 ,  C12N11/10 ,  C12N2533/70 ,  Y10T436/147777

摘要： The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprise the steps consisting of a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide and one cross-linking agent b) freezing the aqueous solution of step a) c) sublimating the frozen solution of step b) characterized in that step b) is performed before the cross-linking of the polysaccharide occurs in the solution of step a).

摘要翻译： 本发明涉及一种用于组织工程的多孔支架的制备方法。 本发明的另一个目的是提供可通过上述方法获得的多孔支架及其在组织工程，细胞培养和细胞递送中的应用。 本发明的方法包括以下步骤：a）制备含有至少一种多糖和一种交联剂的碱性水溶液b）冷冻步骤a）的水溶液c）升华步骤b的冷冻溶液 ），其特征在于步骤b）在多糖的交联发生在步骤a）的溶液中之前进行。

公开(公告)号：US09555164B2

公开(公告)日：2017-01-31

申请号：US14708996

申请日：2015-05-11

申请人： INSERM (Institut National de la Sante et de la Recherche Medicale) ,  Universite Paris 7—Denis Diderot

发明人： Catherine Le Visage ,  Didier Letourneur ,  Frederic Chaubet ,  Aude Autissier

IPC分类号： A61L27/20 ,  A61L27/50 ,  A61L27/56 ,  C08B37/08 ,  A61F13/00 ,  A61L27/38 ,  A61L27/52 ,  C12N5/00 ,  C08B37/00 ,  C08B37/02 ,  C08H1/06 ,  C08J9/28 ,  A61L27/54 ,  C08J3/24 ,  C12N11/10

CPC分类号： A61L27/56 ,  A61L27/20 ,  A61L27/3804 ,  A61L27/3826 ,  A61L27/3834 ,  A61L27/3847 ,  A61L27/3852 ,  A61L27/507 ,  A61L27/52 ,  A61L27/54 ,  A61L2300/21 ,  A61L2430/02 ,  A61L2430/06 ,  C08B37/0018 ,  C08B37/0021 ,  C08B37/003 ,  C08B37/0039 ,  C08B37/0054 ,  C08B37/0063 ,  C08B37/0072 ,  C08B37/0075 ,  C08B37/0084 ,  C08H1/06 ,  C08J3/24 ,  C08J9/28 ,  C08J2201/026 ,  C08J2201/0484 ,  C08J2205/04 ,  C08J2207/10 ,  C08J2305/00 ,  C08J2305/02 ,  C08J2305/08 ,  C08J2305/12 ,  C12N5/0068 ,  C12N11/10 ,  C12N2533/70 ,  Y10T436/147777

摘要： The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprise the steps consisting of a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide and one cross-linking agent b) freezing the aqueous solution of step a) c) sublimating the frozen solution of step b) characterized in that step b) is performed before the cross-linking of the polysaccharide occurs in the solution of step a).

摘要翻译： 本发明涉及一种用于组织工程的多孔支架的制备方法。 本发明的另一个目的是提供可通过上述方法获得的多孔支架及其在组织工程，细胞培养和细胞递送中的应用。 本发明的方法包括以下步骤：a）制备含有至少一种多糖和一种交联剂的碱性水溶液b）冷冻步骤a）的水溶液c）升华步骤b的冷冻溶液 ），其特征在于步骤b）在多糖的交联发生在步骤a）的溶液中之前进行。

公开(公告)号：US20200016294A1

公开(公告)日：2020-01-16

申请号：US16363471

申请日：2019-03-25

申请人： INSERM (Institut National de la Santé et de la Recherche Médicale) ,  UNIVERSITE PARIS 7 - DENIS DIDEROT

发明人： Catherine Le Visage ,  Didier Letourneur

IPC分类号： A61L27/20 ,  A61L27/38 ,  A61L27/50 ,  A61L27/52 ,  A61L27/56 ,  C12N5/00 ,  C08B37/00 ,  C08B37/02 ,  C08B37/08 ,  C08J9/08 ,  C08J9/28 ,  A61L27/54

摘要： The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprises the steps consisting of: a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide, an amount of a cross-linking agent and an amount of a porogen agent b) transforming the solution into a hydrogel by placing said solution at a temperature from about 4° C. to about 80° C. for a sufficient time to allow the cross-linking of said amount of polysaccharide and c) submerging said hydrogel into an aqueous solution d) washing the porous scaffold obtained at step c).