- 专利标题: Method for preparing porous scaffold for tissue engineering, cell culture and cell delivery
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申请号: US16363471申请日: 2019-03-25
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公开(公告)号: US11511016B2公开(公告)日: 2022-11-29
- 发明人: Catherine Le Visage , Didier Letourneur
- 申请人: INSERM (Institut National de la Santé et de la Recherche Médicale) , UNIVERSITE PARIS 7—DENIS DIDEROT
- 申请人地址: FR Paris; FR Paris
- 专利权人: INSERM (Institut National de la Santé et de la Recherche Médicale),UNIVERSITE PARIS 7—DENIS DIDEROT
- 当前专利权人: INSERM (Institut National de la Santé et de la Recherche Médicale),UNIVERSITE PARIS 7—DENIS DIDEROT
- 当前专利权人地址: FR Paris; FR Paris
- 代理机构: WCF IP
- 优先权: EP07301452 20071011
- 主分类号: A61L27/20
- IPC分类号: A61L27/20 ; A61L27/38 ; A61L27/50 ; A61L27/52 ; A61L27/56 ; C12N5/00 ; C08B37/00 ; C08B37/02 ; C08B37/08 ; C08J9/08 ; C08J9/28 ; A61L27/54
摘要:
The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprises the steps consisting of: a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide, an amount of a cross-linking agent and an amount of a porogen agent b) transforming the solution into a hydrogel by placing said solution at a temperature from about 4° C. to about 80° C. for a sufficient time to allow the cross-linking of said amount of polysaccharide and c) submerging said hydrogel into an aqueous solution d) washing the porous scaffold obtained at step c).
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