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    Process for generating a variant library of DNA sequences
    2.
    发明授权
    Process for generating a variant library of DNA sequences 有权

    公开(公告)号：US09856470B2

    公开(公告)日：2018-01-02

    申请号：US12960061

    申请日：2010-12-03

    申请人： Thomas Greiner-Stoeffele ,  Claudia Feller ,  Marc Struhalla

    发明人： Thomas Greiner-Stoeffele ,  Claudia Feller ,  Marc Struhalla

    IPC分类号： C12Q1/68 ,  C12N15/10

    CPC分类号： C12N15/1093 ,  C12N15/102

    摘要： A method for generating a variant library of DNA sequences starting from at least one DNA starting sequence and including the steps: a) selecting at least two mutation sites in the starting sequence; b) dividing the DNA starting sequence into at least two sequence segments such that at least two of these sequence segments each contain at least one of the mutation sites; c) amplifying the sequence segments by polymerase chain reaction using at least five different oligonucleotides, where at (i) least one of the oligonucleotides can attach to each mutation site; (ii) at least two of the oligonucleotides can attach to at least one mutation site, and (iii) mutations are introduced, via mismatch positions, into the PCR amplificates by the oligonucleotides at the mutation sites where at least two mutations are introduced at at least one of the mutation sites; and d) linking the amplificates to give DNA sequences.

    Nitrilases
    5.
    发明授权
    Nitrilases 有权
    腈水解酶

    公开(公告)号：US08916364B2

    公开(公告)日：2014-12-23

    申请号：US13878979

    申请日：2011-10-12

    申请人： Andreas Vogel ,  Daniel Schwarze ,  Thomas Greiner-Stoeffele

    发明人： Andreas Vogel ,  Daniel Schwarze ,  Thomas Greiner-Stoeffele

    IPC分类号： C07K14/195 ,  C12N9/78

    CPC分类号： C12N9/78 ,  C07K14/195 ,  C12P7/40 ,  C12P7/42 ,  C12P13/002 ,  C12Y305/05001

    摘要： The invention relates to a nitrilase having improved activity in the reaction of a nitrile to form the corresponding carboxylic acid, in particular with respect to reacting 2-methylglutaronitrile, 1-(cyanomethyl)cyclohexane-1-carbonitrile, and benzonitrile. The nitrilase according to the invention is related to nitrilase from acidovorax facilis.

    摘要翻译： 本发明涉及在腈的反应中具有改善的活性以形成相应的羧酸的腈水解酶，特别是关于2-甲基戊二腈，1-（氰基甲基）环己烷-1-甲腈和苄腈的反应。 根据本发明的腈水解酶与来自酸酵母的腈水解酶有关。

    Nitrilases
    7.
    发明申请
    Nitrilases 有权
    腈水解酶

    公开(公告)号：US20130288315A1

    公开(公告)日：2013-10-31

    申请号：US13878979

    申请日：2011-10-12

    申请人： Andreas Vogel ,  Daniel Schwarze ,  Thomas Greiner-Stoeffele

    发明人： Andreas Vogel ,  Daniel Schwarze ,  Thomas Greiner-Stoeffele

    IPC分类号： C12N9/78

    CPC分类号： C12N9/78 ,  C07K14/195 ,  C12P7/40 ,  C12P7/42 ,  C12P13/002 ,  C12Y305/05001

    摘要： The invention relates to a nitrilase having improved activity in the reaction of a nitrile to form the corresponding carboxylic acid, in particular with respect to reacting 2-methylglutaronitrile, 1-(cyanomethyl)cyclohexane-1-carbonitrile, and benzonitrile. The nitrilase according to the invention is related to nitrilase from acidovorax facilis.

    摘要翻译： 本发明涉及在腈的反应中具有改善的活性以形成相应的羧酸的腈水解酶，特别是关于2-甲基戊二腈，1-（氰基甲基）环己烷-1-甲腈和苄腈的反应。 根据本发明的腈水解酶与来自酸酵母的腈水解酶有关。

    Process for Generating a Variant Library of DNA Sequences
    9.
    发明申请
    Process for Generating a Variant Library of DNA Sequences 有权
    生成DNA序列变体库的过程

    公开(公告)号：US20110152126A1

    公开(公告)日：2011-06-23

    申请号：US12960061

    申请日：2010-12-03

    申请人： Thomas GREINER-STOEFFELE ,  Claudi FELLER ,  Marc STRUHALLA

    发明人： Thomas GREINER-STOEFFELE ,  Claudi FELLER ,  Marc STRUHALLA

    IPC分类号： C40B40/08 ,  C40B50/06

    CPC分类号： C12N15/1093 ,  C12N15/102

    摘要： A method for generating a variant library of DNA sequences starting from at least one DNA starting sequence and including the following steps: a) selecting at least two mutation sites in the starting sequence; b) dividing the DNA starting sequence into at least two sequence segments in such a way that at least two of these sequence segments each contain at least one of the mutation sites; c) amplifying the sequence segments by polymerase chain reaction with the aid of a total of at least five different oligonucleotides, where at (i) least one of the oligonucleotides can attach to each of the mutation sites; (ii) at least two of the oligonucleotides can attach to at least one mutation site, and (iii) mutations are introduced, via mismatch positions, into the PCR amplificates by the oligonucleotides at the mutation sites where at least two mutations are introduced at least one of the mutation sites; and d) linking the amplificates to give DNA sequences.

    摘要翻译： 一种从至少一个DNA起始序列开始产生DNA序列的变体文库的方法，包括以下步骤：a）选择起始序列中的至少两个突变位点; b）将DNA起始序列分成至少两个序列片段，使得这些序列片段中的至少两个各自含有至少一个突变位点; c）通过总共至少五种不同寡核苷酸的聚合酶链反应扩增序列片段，其中（i）至少一种寡核苷酸可以连接到每个突变位点; （ii）至少两个寡核苷酸可以连接至至少一个突变位点，和（iii）通过错配位置将突变引入至少两个突变至少两个突变的突变位点处的PCR扩增片段 其中一个突变位点; 和d）连接扩增产生DNA序列。