公开(公告)号：US09856470B2

公开(公告)日：2018-01-02

申请号：US12960061

申请日：2010-12-03

申请人： Thomas Greiner-Stoeffele ,  Claudia Feller ,  Marc Struhalla

发明人： Thomas Greiner-Stoeffele ,  Claudia Feller ,  Marc Struhalla

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12N15/1093 ,  C12N15/102

摘要： A method for generating a variant library of DNA sequences starting from at least one DNA starting sequence and including the steps: a) selecting at least two mutation sites in the starting sequence; b) dividing the DNA starting sequence into at least two sequence segments such that at least two of these sequence segments each contain at least one of the mutation sites; c) amplifying the sequence segments by polymerase chain reaction using at least five different oligonucleotides, where at (i) least one of the oligonucleotides can attach to each mutation site; (ii) at least two of the oligonucleotides can attach to at least one mutation site, and (iii) mutations are introduced, via mismatch positions, into the PCR amplificates by the oligonucleotides at the mutation sites where at least two mutations are introduced at at least one of the mutation sites; and d) linking the amplificates to give DNA sequences.

公开(公告)号：US09391348B2

公开(公告)日：2016-07-12

申请号：US13823884

申请日：2011-09-14

申请人： Adrian Goldberg ,  Peter Marcinkowski ,  Claudia Feller ,  Mareike Wolter

发明人： Adrian Goldberg ,  Peter Marcinkowski ,  Claudia Feller ,  Mareike Wolter

IPC分类号： H01M10/48 ,  H01M10/0525

CPC分类号： H01M10/48 ,  H01M10/0525 ,  H01M10/484 ,  H01M10/486 ,  Y02E60/122

摘要： The invention relates to electrochemical cells of a rechargeable battery. It is the object of the invention to provide possibilities with which parameters can be determined simply, reliably and with sufficient spatial resolution within the cells of a rechargeable battery in real time and with small additional technical effort. In an electrochemical cell in accordance with the invention, at least one sensor element is arranged within the cell, integrated therein. In this respect, a sensor element is configured as an electrically conductive layer-wise conductor track structure on a surface of a dielectric laminate configured in the form of a thin film. Except for regions arranged at the outer margin provided for an electrical contacting, the conductor track structure is sealed in a fluid-tight manner by a further dielectric laminate which is configured in the form of a film and which is arranged on the conductor track structure.

摘要翻译： 本发明涉及可再充电电池的电化学电池。 本发明的目的是提供可以简单，可靠地确定哪些参数以及在可再充电电池的单元内实时具有足够的空间分辨率以及小的附加技术努力的可能性。 在根据本发明的电化学电池中，至少一个传感器元件布置在电池内，并集成在其中。 在这方面，传感器元件被构造为在薄膜形式的电介质层叠体的表面上的导电层状导体轨道结构。 除了设置在用于电接触的外边缘处的区域之外，导体轨道结构通过被配置为膜形式并且布置在导体轨道结构上的另外的电介质层压体以流体密封的方式密封。