公开(公告)号：US06261440B1

公开(公告)日：2001-07-17

申请号：US08928364

申请日：1997-09-12

Applicant: Timothy P. Henning ,  Thomas G. Spring

Inventor： Timothy P. Henning ,  Thomas G. Spring

IPC: G01F164

CPC classification number: C12Q1/005 ,  C12Q1/002 ,  Y10S435/817

Abstract: Provided are microparticle forms of carbon, carbon catalysts and carbon-containing electrically conductive materials which are covalently linked to peroxidase. The carbon:peroxidase conjugates are suitable for use as substrates in conventional electrodes. Surprisingly, the conjugates display very little sensitivity to known interfering substances and thus are suitable for use as interference free electrodes.

Abstract translation: 提供了与过氧化物酶共价连接的碳，碳催化剂和含碳导电材料的微粒形式。 碳：过氧化物酶缀合物适用于常规电极中的底物。 令人惊奇的是，缀合物对已知的干扰物质的灵敏度非常小，因此适合用作无干扰电极。

公开(公告)号：US5643721A

公开(公告)日：1997-07-01

申请号：US193972

申请日：1994-02-09

Applicant: Thomas G. Spring ,  John M. Brackett ,  Sheila A. Vogdes ,  Steven G. Schultz

Inventor： Thomas G. Spring ,  John M. Brackett ,  Sheila A. Vogdes ,  Steven G. Schultz

IPC: C12N11/14 ,  C12Q1/25 ,  G01N27/06 ,  G01N33/543 ,  G01N33/545 ,  G01N33/548 ,  G01N33/551

CPC classification number: G01N33/545 ,  G01N33/543 ,  G01N33/5436 ,  Y10S435/817 ,  Y10S436/806

Abstract: The present invention provides an immobilization medium which can immobilize bioreagents to support materials and which dries to a water resistant layer or film. The immobilization medium comprises (i) a liquid or fluid binding reagent and (ii) complexes of a bioreagent immobilized to a solid phase which are evenly dispersed within the binding reagent. The suspension can further include supplemental ingredients evenly dispersed throughout the medium which can provide the medium with electrochemical properties, enhance the stability of the immobilized bioreagent and/or improve the medium's capability of drying to a substantially water resistant or insoluble layer. The immobilization medium provided by the instant invention is in the form of a homogeneous liquid suspension.

Abstract translation: 本发明提供一种固定培养基，其可以将生物试剂固定在支撑材料上并干燥到防水层或膜上。 固定培养基包括（i）液体或流体结合试剂和（ii）固定在固相上的生物试剂的复合物，其均匀分散在结合试剂中。 悬浮液可以进一步包括均匀分散在整个培养基中的补充成分，其可以为培养基提供电化学性质，增强固定化生物试剂的稳定性和/或提高培养基对基本上防水或不溶性层的干燥能力。 由本发明提供的固定介质是均匀的液体悬浮液的形式。

公开(公告)号：US08288111B2

公开(公告)日：2012-10-16

申请号：US12126316

申请日：2008-05-23

Applicant: Elaine M. Brate ,  David M. Finley ,  Shelley R. Holets-McCormack ,  David P. Pacenti ,  Ryan E. Piktel ,  Michelle L. Shields ,  Thomas G. Spring ,  Phillip P. Wang

Inventor： Elaine M. Brate ,  David M. Finley ,  Shelley R. Holets-McCormack ,  David P. Pacenti ,  Ryan E. Piktel ,  Michelle L. Shields ,  Thomas G. Spring ,  Phillip P. Wang

IPC: G01N33/53

CPC classification number: G01N33/54393 ,  G01N33/9493

Abstract: The present disclosure provides among other things immunoassays exhibiting reduced cross-reactivity with analyte metabolites. Additionally, the present disclosure provides diagnostic immunoassays to determine the concentration or level in a test sample of a hydrophobic drug that metabolizes in vivo or in vitro to form cross-reacting metabolites wherein cross-reactivity with such metabolites of the drug analyte is reduced. In particular, the disclosure provides such immunoassays where the hydrophobic drug is an immunosuppressant drug such as cyclosporine A.

Abstract translation: 本公开提供了与分析物代谢物显示降低的交叉反应性的免疫测定法。 此外，本公开提供诊断免疫测定以确定在体内或体外代谢形成交叉反应代谢物的疏水性药物的测试样品中的浓度或水平，其中与药物分析物的这种代谢物的交叉反应性降低。 特别地，本公开提供了这样的免疫测定，其中疏水性药物是免疫抑制剂药物，例如环孢菌素A.

公开(公告)号：US5755953A

公开(公告)日：1998-05-26

申请号：US563728

申请日：1995-12-18

Applicant: Timothy P. Henning ,  Thomas G. Spring

Inventor： Timothy P. Henning ,  Thomas G. Spring

IPC: G01N27/327 ,  C12Q1/00 ,  C12Q1/28 ,  C12Q1/54 ,  G01N27/416 ,  G01N27/26

CPC classification number: C12Q1/002 ,  Y10S435/817

Abstract: Provided are microparticle forms of carbon, carbon catalysts and carbon-containing electrically conductive materials which are covalently linked to peroxidase. The carbon:peroxidase conjugates are suitable for use as substrates in conventional electrodes. Surprisingly, the conjugates display very little sensitivity to known interfering substances and thus are suitable for use as interference free electrodes.

Abstract translation: 提供了与过氧化物酶共价连接的碳，碳催化剂和含碳导电材料的微粒形式。 碳：过氧化物酶缀合物适用于常规电极中的底物。 令人惊奇的是，缀合物对已知的干扰物质的灵敏度非常小，因此适合用作无干扰电极。

公开(公告)号：US20080311676A1

公开(公告)日：2008-12-18

申请号：US12126316

申请日：2008-05-23

Applicant: Elaine M. Brate ,  David M. Finley ,  Shelley R. Holets-McCormack ,  David P. Pacenti ,  Ryan E. Piktel ,  Michelle Shields ,  Thomas G. Spring ,  Philip P. Wang

Inventor： Elaine M. Brate ,  David M. Finley ,  Shelley R. Holets-McCormack ,  David P. Pacenti ,  Ryan E. Piktel ,  Michelle Shields ,  Thomas G. Spring ,  Philip P. Wang

IPC: G01N33/566

CPC classification number: G01N33/54393 ,  G01N33/9493

Abstract: The present disclosure provides among other things immunoassays exhibiting reduced cross-reactivity with analyte metabolites. Additionally, the present disclosure provides diagnostic immunoassays to determine the concentration or level in a test sample of a hydrophobic drug that metabolizes in vivo or in vitro to form cross-reacting metabolites wherein cross-reactivity with such metabolites of the drug analyte is reduced. In particular, the disclosure provides such immunoassays where the hydrophobic drug is an immunosuppressant drug such as cyclosporine A.

Abstract translation: 本公开提供了与分析物代谢物显示降低的交叉反应性的免疫测定法。 此外，本公开提供诊断免疫测定以确定在体内或体外代谢形成交叉反应代谢物的疏水性药物的测试样品中的浓度或水平，其中与药物分析物的这种代谢物的交叉反应性降低。 特别地，本公开提供了这样的免疫测定，其中疏水性药物是免疫抑制剂药物，例如环孢菌素A.

公开(公告)号：US07256008B2

公开(公告)日：2007-08-14

申请号：US11327711

申请日：2006-01-06

Applicant: Thomas G. Spring ,  Elaine M. Brate ,  Shelley Holets-McCormack ,  Rajarathnam E. Reddy ,  Donald D. Johnson ,  Yon-Yih Chen ,  You Pan

Inventor： Thomas G. Spring ,  Elaine M. Brate ,  Shelley Holets-McCormack ,  Rajarathnam E. Reddy ,  Donald D. Johnson ,  Yon-Yih Chen ,  You Pan

IPC: G01N33/00

CPC classification number: G01N33/9493

Abstract: Methods and kits for measurement of concentration of FK778 in a biological sample by means of an immunoassay, preferably a competitive immunoassay. In one aspect, the method and kit involve the use of (a) an antibody to FK778 conjugated to a label, e.g., an acridinium label, (b) an antibody to FK778 not conjugated to a label, (c) a solid phase containing an antibody to a first hapten, e.g., a fluorescein hapten, and (d) a bihapten comprising a first hapten and FK778 or an analogue of FK778, e.g., a bihapten comprising a fluorescein hapten and a FK778 hapten. In another aspect, the method and kit involve the use of (a) antibody to FK778, (b) a bihapten comprising FK778 or an analogue of FK778 and a first hapten, e.g., a bihapten comprising the fluorescein hapten and the hapten of FK778 or an analogue of FK778, and (c) a pretreatment reagent.

Abstract translation: 通过免疫测定，优选竞争性免疫测定法测量生物样品中FK778的浓度的方法和试剂盒。 一方面，该方法和试剂盒涉及使用（a）与标记缀合的FK778的抗体，例如吖啶标记，（b）与标记物不缀合的FK778的抗体，（c）含有 第一半抗原的抗体，例如荧光素半抗原，和（d）包含第一半抗原和FK778或FK778的类似物的双突变体，例如包含荧光素半抗原和FK778半抗原的双突变体。 另一方面，该方法和试剂盒涉及使用（a）FK778的抗体，（b）包含FK778或类似物FK778的双突变体和第一半抗原，例如包含荧光素半抗原的双突变体和FK778的半抗原或 FK778的类似物，（c）预处理试剂。

公开(公告)号：US4476228A

公开(公告)日：1984-10-09

申请号：US440068

申请日：1982-11-08

Applicant: Roy F. Huchzermeier ,  Thomas G. Spring

Inventor： Roy F. Huchzermeier ,  Thomas G. Spring

IPC: G01N33/566 ,  C07D311/82 ,  C07D493/10 ,  G01N33/78 ,  G01N3/52

CPC classification number: C07D493/10 ,  C07D311/82 ,  G01N33/78 ,  Y10S436/80

Abstract: This invention encompasses a method for measuring unsaturated thyroxine binding protein sites in a sample comprising intermixing with the sample an effective amount of fluorescent labeled tracer having binding affinity for thyroxine binding proteins and then measuring the amount of tracer bound to the thyroxine binding protein using fluorescence polarization techniques.

Abstract translation: 本发明包括用于测量样品中不饱和甲状腺素结合蛋白位点的方法，包括与样品混合有效量的对甲状腺结合蛋白具有结合亲和力的荧光标记示踪物，然后使用荧光偏振测量与甲状腺素结合蛋白结合的示踪剂的量 技术