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公开(公告)号：US08211446B2
公开(公告)日：2012-07-03
申请号：US12870952
申请日：2010-08-30
Applicant: Pappachan E. Kolattukudy , Tatiana Sirakova , Jaiyanth Daniel , Chirajyoti Deb
Inventor： Pappachan E. Kolattukudy , Tatiana Sirakova , Jaiyanth Daniel , Chirajyoti Deb
Abstract: A method of inducing latency in Mycobacterium permits preparation of an in vitro model system of latent mycobacterial infection. Latency is induced in a pure culture of Mycobacterium by exposing it to multiple stress conditions, including a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide and a relatively low gas phase oxygen level. An in vitro model of mycobacterial infection employs macrophages induced from THP1 cells which are then infected with Mycobacterium. The infected macrophages are grown under hypoxic conditions to induce latency in the mycobacteria. The in vitro model of infection is useful in evaluating compounds for activity against latent mycobacteria.
Abstract translation: 在分枝杆菌中诱导潜伏期的方法允许制备潜在分枝杆菌感染的体外模型系统。 通过将其暴露于多种胁迫条件，包括不含甘油的低营养培养基，低pH值，相对高水平的二氧化碳和相对低的气相氧水平，在纯分枝杆菌的纯培养物中诱导延迟。 分支杆菌感染的体外模型使用由THP1细胞诱导的巨噬细胞，然后用分枝杆菌感染。 感染的巨噬细胞在缺氧条件下生长以在分枝杆菌中诱导潜伏期。 感染的体外模型可用于评价化合物对潜在分枝杆菌的活性。
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公开(公告)号：US20090023596A1
公开(公告)日：2009-01-22
申请号：US12107146
申请日：2008-04-22
Applicant: Pappachan Kolattukudy , Tatiana Sirakova , Jaiyanth Daniel , Chirajyoti Deb
Inventor： Pappachan Kolattukudy , Tatiana Sirakova , Jaiyanth Daniel , Chirajyoti Deb
Abstract: A method of inducing latency in Mycobacterium permits preparation of an in vitro model system of latent mycobacterial infection. Latency is induced in a pure culture of Mycobacterium by exposing it to multiple stress conditions, including a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide and a relatively low gas phase oxygen level. An in vitro model of mycobacterial infection employs macrophages induced from THP1 cells which are then infected with Mycobacterium. The infected macrophages are grown under hypoxic conditions to induce latency in the mycobacteria. The in vitro model of infection is useful in evaluating compounds for activity against latent mycobacteria.
Abstract translation: 在分枝杆菌中诱导潜伏期的方法允许制备潜在分枝杆菌感染的体外模型系统。 通过将其暴露于多种胁迫条件，包括不含甘油的低营养培养基，低pH值，相对高水平的二氧化碳和相对低的气相氧水平，在纯分枝杆菌的纯培养物中诱导延迟。 分支杆菌感染的体外模型使用由THP1细胞诱导的巨噬细胞，然后用分枝杆菌感染。 感染的巨噬细胞在缺氧条件下生长以在分枝杆菌中诱导潜伏期。 感染的体外模型可用于评价化合物对潜在分枝杆菌的活性。
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3.发明申请DIFFERENTIAL TARGETING OF TRIACYLGLYCEROL SYNTHASE AND WAX SYNTHASE ACTIVITY OF THE TRIACYLGLYCEROL SYNTHASE GENE FOR TUBERCULOSIS TREATMENT 审中-公开三氯乙烯合成酶基因的三联体合成酶和WAX合成酶活性的差异性鉴定用于管状细胞治疗公开(公告)号：US20110190479A1
公开(公告)日：2011-08-04
申请号：US12983704
申请日：2011-01-03
Applicant: PAPPACHAN E. KOLATTUKUDY , TATIANA SIRAKOVA , VINOD S. DUBEY , JAIYANTH DANIEL
Inventor： PAPPACHAN E. KOLATTUKUDY , TATIANA SIRAKOVA , VINOD S. DUBEY , JAIYANTH DANIEL
Abstract: The application reports the discovery that mycobacterial triacylglycerol synthase (MTTGS) enzymes also have a wax synthase function. This bifunctional enzymatic activity, or ‘dual function,’ implies that the enzymes contain separate binding sites for diacylglycerol and acyl alcohol and a common binding site for acyl-coenzyme A. Embodiments of the invention relate to methods of selection and/or design of therapeutic compounds that will reduce any potentially toxic side effects of candidate drugs designed to inhibit the mycobacterial enzyme.
Abstract translation: 该应用报告了分枝杆菌三酰基甘油合酶（MTTGS）酶也具有蜡合酶功能的发现。 这种双功能酶活性或“双重功能”意味着酶含有二酰基甘油和酰基醇的单独结合位点和酰基辅酶A的常见结合位点。本发明的实施方案涉及治疗性选择和/或设计的方法 将减少旨在抑制分枝杆菌酶的候选药物的任何潜在毒性副作用的化合物。
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4.发明申请公开(公告)号：US20080014206A1
公开(公告)日：2008-01-17
申请号：US11561477
申请日：2006-11-20
Applicant: Pappachan Kolattukudy , Tatiana Sirakova , Vinod Dubey
Inventor： Pappachan Kolattukudy , Tatiana Sirakova , Vinod Dubey
CPC classification number: G01N33/5695 , A61K38/1709 , G01N2500/04 , G01N2500/10
Abstract: Disclosed herein are novel methods for screening for compounds useful in treating or preventing tuberculosis. In exemplary embodiments, screening methods are based on the implementation or manipulation of triacylglycerol synthase like polypeptides or polynucleotides encoding the same. The methods are useful in identifying agents active against TB infection.
Abstract translation: 本文公开了用于筛选用于治疗或预防结核病的化合物的新方法。 在示例性实施方案中，筛选方法基于三酰基甘油合酶如多肽或编码其的多核苷酸的实施或操作。 该方法可用于鉴定对结核感染有活性的药剂。
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公开(公告)号：US6030626A
公开(公告)日：2000-02-29
申请号：US467722
申请日：1995-06-06
Applicant: Pappachan E. Kolattukudy , Lauren O. Bakaletz , Tatiana Sirakova
Inventor： Pappachan E. Kolattukudy , Lauren O. Bakaletz , Tatiana Sirakova
IPC: C12N1/21 , A61K39/00 , A61K39/102 , A61P31/04 , C07K14/08 , C07K14/285 , C12N5/10 , C12N7/00 , C12N15/09 , C12N15/31 , C12P21/02 , C12R1/01 , C12R1/19 , C12P21/06 , C12Q1/68 , G01N33/574
CPC classification number: C07K14/285 , A61K39/00
Abstract: It has been discovered that a vaccine comprised of fimbrin, a filamentous protein derived from the bacterial surface appendages of non-typable Haemophilus influenzae is useful in studying, preventing or reducing the severity of, otitis media. The gene sequence of the DNA coding for fimbrin and the amino acid sequence of fimbrin have also been determined. Vectors containing DNA coding for fimbrin have also been developed, and transformants have been prepared which contain such vectors and which express such DNA and provide a source of pure fimbrin.
Abstract translation: 已经发现，由fimbrin组成的疫苗，来自非特异性流感嗜血杆菌的细菌表面附着物的丝状蛋白质可用于研究，预防或降低中耳炎的严重性。 还测定了编码fimbrin的DNA的基因序列和fimbrin的氨基酸序列。 还开发了含有编码fimbrin的DNA的载体，并且已经制备了含有这种载体并且表达这种DNA并提供纯Fimbrin来源的转化体。
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Patent Agency Ranking
公开(公告)号：US08241644B2
公开(公告)日：2012-08-14
申请号：US12870945
申请日：2010-08-30
Applicant: Pappachan E. Kolattukudy , Tatiana Sirakova , Jaiyanth Daniel , Chirajyoti Deb
Inventor： Pappachan E. Kolattukudy , Tatiana Sirakova , Jaiyanth Daniel , Chirajyoti Deb
Abstract: A method of inducing latency in Mycobacterium permits preparation of an in vitro model system of latent mycobacterial infection. Latency is induced in a pure culture of Mycobacterium by exposing it to multiple stress conditions, including a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide and a relatively low gas phase oxygen level. An in vitro in vitro model of mycobacterial infection employs macrophages induced from THP1 cells which are then infected with Mycobacterium. The infected macrophages are grown under hypoxic conditions to induce latency in the mycobacteria. The in vitro model of infection is useful in evaluating compounds for activity against latent mycobacteria.
Abstract translation: 在分枝杆菌中诱导潜伏期的方法允许制备潜在分枝杆菌感染的体外模型系统。 通过将其暴露于多种胁迫条件，包括不含甘油的低营养培养基，低pH值，相对高水平的二氧化碳和相对低的气相氧水平，在纯分枝杆菌的纯培养物中诱导延迟。 分支杆菌感染的体外体外模型使用由THP1细胞诱导的巨噬细胞，然后用分枝杆菌感染。 感染的巨噬细胞在缺氧条件下生长以在分枝杆菌中诱导潜伏期。 感染的体外模型可用于评价化合物对潜在分枝杆菌的活性。
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公开(公告)号：US20110033914A1
公开(公告)日：2011-02-10
申请号：US12870945
申请日：2010-08-30
Applicant: Pappachan Kolattukudy , Tatiana Sirakova , Jaiyanth Daniel , Chirajyoti Deb
Inventor： Pappachan Kolattukudy , Tatiana Sirakova , Jaiyanth Daniel , Chirajyoti Deb
IPC: C12N1/20
Abstract: A method of inducing latency in Mycobacterium permits preparation of an in vitro model system of latent mycobacterial infection. Latency is induced in a pure culture of Mycobacterium by exposing it to multiple stress conditions, including a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide and a relatively low gas phase oxygen level. An in vitro model of mycobacterial infection employs macrophages induced from THP1 cells which are then infected with Mycobacterium. The infected macrophages are grown under hypoxic conditions to induce latency in the mycobacteria. The in vitro model of infection is useful in evaluating compounds for activity against latent mycobacteria.
Abstract translation: 在分枝杆菌中诱导潜伏期的方法允许制备潜在分枝杆菌感染的体外模型系统。 通过将其暴露于多种胁迫条件，包括不含甘油的低营养培养基，低pH值，相对高水平的二氧化碳和相对低的气相氧水平，在纯分枝杆菌的纯培养物中诱导延迟。 分支杆菌感染的体外模型使用由THP1细胞诱导的巨噬细胞，然后用分枝杆菌感染。 感染的巨噬细胞在缺氧条件下生长以在分枝杆菌中诱导潜伏期。 感染的体外模型可用于评价化合物对潜在分枝杆菌的活性。
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公开(公告)号：US20130040829A1
公开(公告)日：2013-02-14
申请号：US13572888
申请日：2012-08-13
Applicant: Pappachan E. Kolattukudy , Tatiana Sirakova , Jaiyanth Daniel , Chirajoti Deb
Inventor： Pappachan E. Kolattukudy , Tatiana Sirakova , Jaiyanth Daniel , Chirajoti Deb
Abstract: A method of inducing latency in Mycobacterium permits preparation of an in vitro model system of latent mycobacterial infection. Latency is induced in a pure culture of Mycobacterium by exposing it to multiple stress conditions, including a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide and a relatively low gas phase oxygen level. An in vitro model of mycobacterial infection employs macrophages induced from THP1 cells which are then infected with Mycobacterium. The infected macrophages are grown under hypoxic conditions to induce latency in the mycobacteria. The in vitro model of infection is useful in evaluating compounds for activity against latent mycobacteria.
Abstract translation: 在分枝杆菌中诱导潜伏期的方法允许制备潜在分枝杆菌感染的体外模型系统。 通过将其暴露于多种胁迫条件，包括不含甘油的低营养培养基，低pH值，较高水平的二氧化碳和相对低的气相氧水平，在纯分枝杆菌的纯培养物中诱导潜伏期。 分支杆菌感染的体外模型使用由THP1细胞诱导的巨噬细胞，然后用分枝杆菌感染。 感染的巨噬细胞在缺氧条件下生长以在分枝杆菌中诱导潜伏期。 感染的体外模型可用于评价化合物对潜在分枝杆菌的活性。
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公开(公告)号：US08012492B2
公开(公告)日：2011-09-06
申请号：US12107146
申请日：2008-04-22
Applicant: Pappachan Kolattukudy , Tatiana Sirakova , Jaiyanth Daniel , Chirajyoti Deb
Inventor： Pappachan Kolattukudy , Tatiana Sirakova , Jaiyanth Daniel , Chirajyoti Deb
Abstract: A method of inducing latency in Mycobacterium permits preparation of an in vitro model system of latent mycobacterial infection. Latency is induced in a pure culture of Mycobacterium by exposing it to multiple stress conditions, including a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide and a relatively low gas phase oxygen level. An in vitro model of mycobacterial infection employs macrophages induced from THP1 cells which are then infected with Mycobacterium. The infected macrophages are grown under hypoxic conditions to induce latency in the mycobacteria. The in vitro model of infection is useful in evaluating compounds for activity against latent mycobacteria.
Abstract translation: 在分枝杆菌中诱导潜伏期的方法允许制备潜在分枝杆菌感染的体外模型系统。 通过将其暴露于多种胁迫条件，包括不含甘油的低营养培养基，低pH值，相对高水平的二氧化碳和相对低的气相氧水平，在纯分枝杆菌的纯培养物中诱导延迟。 分支杆菌感染的体外模型使用由THP1细胞诱导的巨噬细胞，然后用分枝杆菌感染。 感染的巨噬细胞在缺氧条件下生长以在分枝杆菌中诱导潜伏期。 感染的体外模型可用于评价化合物对潜在分枝杆菌的活性。
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10.发明申请公开(公告)号：US20100021453A1
公开(公告)日：2010-01-28
申请号：US12546368
申请日：2009-08-24
Applicant: Pappachan E. Kolattukudy , Tatiana Sirakova , Vinod S. Dubey , Jaiyanth Daniel
Inventor： Pappachan E. Kolattukudy , Tatiana Sirakova , Vinod S. Dubey , Jaiyanth Daniel
IPC: A61K39/395 , C07H21/04 , C07K14/35 , A61K48/00 , A61P31/04 , A61K38/00 , C12N15/63 , C12N1/21 , C12N1/19 , C12N5/10
CPC classification number: G01N33/5695 , A61K38/1709 , G01N2500/04 , G01N2500/10
Abstract: Disclosed herein are novel methods for screening for compounds useful in treating or preventing tuberculosis. In exemplary embodiments, screening methods are based on the implementation or manipulation of triacylglycerol synthase like polypeptides or polynucleotides encoding the same. The methods are useful in identifying agents active against TB infection.
Abstract translation: 本文公开了用于筛选用于治疗或预防结核病的化合物的新方法。 在示例性实施方案中，筛选方法基于三酰基甘油合酶如多肽或编码其的多核苷酸的实施或操作。 该方法可用于鉴定对结核感染有活性的药剂。
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