公开(公告)号：US5750330A

公开(公告)日：1998-05-12

申请号：US666134

申请日：1996-06-19

申请人： Andrew M. Tometsko, deceased ,  Stephen Dertinger ,  Dorothea Torous ,  Kenneth Tometsko

发明人： Andrew M. Tometsko, deceased ,  Stephen Dertinger ,  Dorothea Torous ,  Kenneth Tometsko

IPC分类号： A01N1/02

CPC分类号： A01N1/02 ,  A01N1/0221

摘要： Disclosed are a composition for the lyophilization of mammalian red blood cells comprising a hydrophilic polymer, a carbohydrate, and an organic solvent; and a method of using the composition to lyophilize red blood cells comprising mixing red blood cells with the composition, freezing the mixture, and drying the mixture by removing water by sublimation. Also disclosed are red blood cells lyophilized according to this method for lyophilization, and a method for reconstituting the lyophilized red blood cells. In particular, the composition used to lyophilize the red blood cells comprises a mixture of a hydrophilic polymer ranging from 1,450-20,000 Daltons at 5-50% w/v, a mono- or disaccharide or a mixture thereof from 0.01-0.2M and an organic solvent such as a primary alcohol, a secondary alcohol, dimethyl sulfoxide or combinations thereof at 0.5-20% v/v.

摘要翻译： 公开了用于冻干哺乳动物红细胞的组合物，其包含亲水性聚合物，碳水化合物和有机溶剂; 以及使用该组合物冻干红细胞的方法，包括将红细胞与组合物混合，冷冻该混合物，并通过升华除去水来干燥该混合物。 还公开了根据这种冻干方法冻干的红细胞，以及用于重构冻干的红细胞的方法。 特别地，用于冻干红细胞的组合物包括在5-50％w / v范围内为1,450-20,000道尔顿的亲水性聚合物，0.01-0.2M的单糖或二糖或其混合物的混合物，和 有机溶剂如伯醇，仲醇，二甲基亚砜或其组合在0.5-20％v / v。

公开(公告)号：US20060040291A1

公开(公告)日：2006-02-23

申请号：US11166433

申请日：2005-06-24

申请人： Stephen Dertinger ,  Sian Cairns ,  Svetlana Avlasevich ,  Dorothea Torous

发明人： Stephen Dertinger ,  Sian Cairns ,  Svetlana Avlasevich ,  Dorothea Torous

IPC分类号： C12Q1/68 ,  G01N1/30

CPC分类号： C12Q1/68 ,  G01N1/30 ,  G01N15/1475

摘要： The present invention relates a method for the enumeration of mammalian cell micronuclei, while distinguishing micronuclei from the chromatin of dead and dying cells. The method utilizes differential staining of chromatin from dead and dying cells, to distinguish the chromatin from micronuclei and nuclei that can be detected based upon fluorescent emission and light scatter following exposure to an excitatory light source. Counting of micronuclei events relative to the number of nuclei can be used to assess the DNA-damaging potential of a chemical agent, the DNA-damaging potential of a physical agent, the effects of an agent which can modify endogenously-induced DNA damage, and the effects of an agent which can modify exogenously-induced DNA damage. Kits for practicing the invention are also disclosed.

摘要翻译： 本发明涉及一种用于计数哺乳动物细胞微核的方法，同时将微核与死亡和死亡细胞的染色质区分开。 该方法利用来自死亡和死亡细胞的染色质的差异染色，以区分染色质与微核和核，其可以在暴露于兴奋性光源之后基于荧光发射和光散射而被检测。 可以使用相对于核数的微核事件的计数来评估化学试剂的DNA损伤潜能，物理试剂的DNA损伤潜能，可改变内源诱导的DNA损伤的试剂的作用，以及 可以改变外源诱导的DNA损伤的药剂的作用。 还公开了用于实施本发明的试剂盒。

公开(公告)号：US5858667A

公开(公告)日：1999-01-12

申请号：US706680

申请日：1996-09-06

申请人： Stephen Dertinger ,  Dorothea Torous ,  Kenneth Tometsko

发明人： Stephen Dertinger ,  Dorothea Torous ,  Kenneth Tometsko

IPC分类号： G01N33/569 ,  G01N33/80 ,  C12Q1/68 ,  C07H21/02 ,  C12N15/00 ,  G01N33/53

CPC分类号： G01N33/56966 ,  G01N33/80 ,  Y10T436/143333

摘要： A single laser flow cytometric method for the enumeration of micronuclei in erythrocyte populations, wherein a sample of peripheral blood or bone marrow is obtained and the cell populations in the sample are fixed. Reticulocytes in the fixed samples are treated simultaneously with RNAse and with a fluorescent labeled antibody having binding specificity for a surface marker for erythroblasts/reticulocytes. The erythrocyte populations are then stained with a nucleic acid stain which stains DNA representing micronuclei, if present. The stained and/or labelled erythrocyte populations are then exposed to a laser beam of appropriate excitation wavelength for both the nucleic acid staining dye and the fluorescent label to produce fluorescent emission. The fluorescent emission and light scatter produced by the erythrocyte populations are detected by the flow cytometer from which is calculated the number of specific erythrocyte populations in said sample.

摘要翻译： 用于计数红细胞群体中的微核的单一激光流式细胞术方法，其中获得外周血或骨髓样品，并且固定样品中的细胞群。 固定样品中的网织红细胞与RNA酶同时处理，荧光标记抗体对于成红细胞/网织红细胞的表面标记具有结合特异性。 然后用存在代表微核的DNA的核酸染色剂染色红细胞群体（如果存在）。 然后将染色和/或标记的红细胞群体暴露于具有适当激发波长的激光束，用于核酸染色染料和荧光标记以产生荧光发射。 通过流式细胞仪检测由红细胞群体产生的荧光发射和光散射，计算所述样品中特异性红细胞群体的数量。

公开(公告)号：US20050026197A1

公开(公告)日：2005-02-03

申请号：US10878456

申请日：2004-06-28

申请人： Stephen Dertinger

发明人： Stephen Dertinger

IPC分类号： G01N33/80 ,  C12Q1/68 ,  G01N33/567

CPC分类号： G01N33/56966 ,  G01N33/5094 ,  G01N33/80 ,  G01N2333/70582 ,  G01N2333/70596 ,  Y02A50/58 ,  Y10S435/968 ,  Y10S435/973 ,  Y10T436/101666

摘要： A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample. In particular, the use of the second antibody prevents interference by platelet-associated aggregates in the scoring procedures.

摘要翻译： 用于计数微核红细胞群体同时区分血小板和血小板相关聚集体的方法涉及使用具有对网织红细胞表面标志物具有结合特异性的第一荧光标记抗体，对于血小板表面标志物具有结合特异性的第二荧光标记抗体 ，以及在红细胞群体中染色DNA（微核）的核酸染色染料。 因为第一和第二荧光标记的抗体的荧光发射光谱基本上彼此不重叠或者与核酸染色染料的发射光谱基本重叠，所以在标记和染料激发时，可以检测荧光发射和光散射 由红细胞群体和血小板产生，并计数所述样品中一种或多种红细胞群体的细胞数。 特别地，使用第二抗体可防止血小板相关聚集体在评分程序中的干扰。

公开(公告)号：US20080217784A1

公开(公告)日：2008-09-11

申请号：US11875711

申请日：2007-10-19

申请人： Florian Binder ,  Stephen Dertinger ,  Barbara Hasler ,  Alfred Martin ,  Grit Sommer ,  Holger Torwesten

发明人： Florian Binder ,  Stephen Dertinger ,  Barbara Hasler ,  Alfred Martin ,  Grit Sommer ,  Holger Torwesten

IPC分类号： H01L23/48 ,  H01L21/44

CPC分类号： H01L21/76898 ,  H01L21/486 ,  H01L24/12 ,  H01L24/16 ,  H01L24/28 ,  H01L24/33 ,  H01L24/48 ,  H01L24/73 ,  H01L2224/16145 ,  H01L2224/16225 ,  H01L2224/16235 ,  H01L2224/32145 ,  H01L2224/32225 ,  H01L2224/48091 ,  H01L2224/48227 ,  H01L2224/48235 ,  H01L2224/48472 ,  H01L2224/73265 ,  H01L2924/00011 ,  H01L2924/00014 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01019 ,  H01L2924/0102 ,  H01L2924/01023 ,  H01L2924/01024 ,  H01L2924/01029 ,  H01L2924/0103 ,  H01L2924/01033 ,  H01L2924/01047 ,  H01L2924/0105 ,  H01L2924/01058 ,  H01L2924/01072 ,  H01L2924/01073 ,  H01L2924/01074 ,  H01L2924/01078 ,  H01L2924/01079 ,  H01L2924/01082 ,  H01L2924/0132 ,  H01L2924/04941 ,  H01L2924/078 ,  H01L2924/09701 ,  H01L2924/10253 ,  H01L2924/10329 ,  H01L2924/14 ,  H01L2924/15311 ,  H01L2924/15787 ,  H01L2924/181 ,  H01L2924/19041 ,  H01L2924/30107 ,  H01L2924/00 ,  H01L2924/00012 ,  H01L2224/0401 ,  H01L2224/45099 ,  H01L2224/45015 ,  H01L2924/207

摘要： A substrate has at least one feedthrough with at least one channel from a first main surface of the substrate to a second main surface of the substrate. The at least one channel is closed off with a first material. The at least one closed-off channel is filled with an electrically conductive second material.

摘要翻译： 衬底具有至少一个馈通，其具有从衬底的第一主表面到衬底的第二主表面的至少一个通道。 至少一个通道用第一材料封闭。 至少一个闭合通道填充有导电的第二材料。