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公开(公告)号：US07303869B2
公开(公告)日：2007-12-04
申请号：US10269222
申请日：2002-10-11
申请人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd M. Smith
发明人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd M. Smith
CPC分类号： C12Q1/683 , C12Q2561/109 , C12Q2565/101
摘要： The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5′ nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列变异的系统，组合物和方法。 本发明涉及将核酸连接到固体支持物和修饰核酸的方法。 例如，在一些实施方案中，切割剂的5'核酸酶活性用于切割在固体支持物上形成的切割结构，裂解事件的发生表明存在特定的核酸序列。
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公开(公告)号：US20060127949A1
公开(公告)日：2006-06-15
申请号：US11233913
申请日：2005-09-23
CPC分类号： G01N33/6845 , C07K16/00 , C07K2317/567 , C07K2317/569
摘要： A construct is provided that is capable of binding a plurality of molecules, the construct comprising a first moiety with a first molecule binding region and a first molecule non-binding region; and a second moiety with a second molecule-binding region and a second molecule-non-binding region, whereby the first binding region and second binding region are at opposite ends of the construct. Also provided is a method for detecting protein having certain amino acid sequences, the method comprising supplying a collection of proteins each with unknown amino acid sequences, contacting the collection with a moiety having a plurality of binding sites capable of binding with the protein having certain amino acid sequences so as to form a moiety-protein complex, and mixing the complex with a marker specific for the moiety in an amount sufficient to indicate existence of the complex
摘要翻译： 提供能够结合多个分子的构建体，所述构建体包含具有第一分子结合区域和第一分子非结合区域的第一部分; 和具有第二分子结合区和第二分子 - 非结合区的第二部分，其中第一结合区和第二结合区位于构建体的相对端。 还提供了一种用于检测具有某些氨基酸序列的蛋白质的方法，所述方法包括提供各自具有未知氨基酸序列的蛋白质的集合，使所述收集物与具有多个能够与具有某些氨基酸的蛋白质结合的结合位点的部分接触 酸序列，以便形成部分 - 蛋白质复合物，并将复合物与足以表明复合物存在的量的该部分特异性标记物混合
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公开(公告)号：US07659083B2
公开(公告)日：2010-02-09
申请号：US12199683
申请日：2008-08-27
申请人： Fred J. Stevens , Marianne Schiffer , Priscilla Wilkins-Stevens , W. Carey Hanly , Sandra L. Tollaksen
发明人： Fred J. Stevens , Marianne Schiffer , Priscilla Wilkins-Stevens , W. Carey Hanly , Sandra L. Tollaksen
IPC分类号： G01N33/00
CPC分类号： G01N33/6845 , C07K16/00 , C07K2317/567 , C07K2317/569
摘要： A construct is provided that is capable of binding a plurality of molecules, the construct comprising a first moiety with a first molecule binding region and a first molecule non-binding region; and a second moiety with a second molecule-binding region and a second molecule-non-binding region, whereby the first binding region and second binding region are at opposite ends of the construct. Also provided is a method for detecting protein having certain amino acid sequences, the method comprising supplying a collection of proteins each with unknown amino acid sequences, contacting the collection with a moiety having a plurality of binding sites capable of binding with the protein having certain amino acid sequences so as to form a moiety-protein complex, and mixing the complex with a marker specific for the moiety in an amount sufficient to indicate existence of the complex.
摘要翻译： 提供能够结合多个分子的构建体，所述构建体包含具有第一分子结合区域和第一分子非结合区域的第一部分; 和具有第二分子结合区和第二分子 - 非结合区的第二部分，其中第一结合区和第二结合区位于构建体的相对端。 还提供了一种用于检测具有某些氨基酸序列的蛋白质的方法，所述方法包括提供各自具有未知氨基酸序列的蛋白质的集合，使所述收集物与具有多个能够与具有某些氨基酸的蛋白质结合的结合位点的部分接触 酸序列，以便形成部分 - 蛋白质复合物，并将该复合物与足以指示复合物存在的量的该部分特异性标记混合。
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公开(公告)号：US20080319172A1
公开(公告)日：2008-12-25
申请号：US12199683
申请日：2008-08-27
申请人： Fred J. Stevens , Marianne Schiffer , Priscilla Wilkins-Stevens , W. Carey Hanly , Sandra L. Tollaksen
发明人： Fred J. Stevens , Marianne Schiffer , Priscilla Wilkins-Stevens , W. Carey Hanly , Sandra L. Tollaksen
IPC分类号： C07K16/00
CPC分类号： G01N33/6845 , C07K16/00 , C07K2317/567 , C07K2317/569
摘要： A construct is provided that is capable of binding a plurality of molecules, the construct comprising a first moiety with a first molecule binding region and a first molecule non-binding region; and a second moiety with a second molecule-binding region and a second molecule-non-binding region, whereby the first binding region and second binding region are at opposite ends of the construct. Also provided is a method for detecting protein having certain amino acid sequences, the method comprising supplying a collection of proteins each with unknown amino acid sequences, contacting the collection with a moiety having a plurality of binding sites capable of binding with the protein having certain amino acid sequences so as to form a moiety-protein complex, and mixing the complex with a marker specific for the moiety in an amount sufficient to indicate existence of the complex
摘要翻译： 提供能够结合多个分子的构建体，所述构建体包含具有第一分子结合区域和第一分子非结合区域的第一部分; 和具有第二分子结合区和第二分子 - 非结合区的第二部分，其中第一结合区和第二结合区位于构建体的相对端。 还提供了一种用于检测具有某些氨基酸序列的蛋白质的方法，所述方法包括提供各自具有未知氨基酸序列的蛋白质的集合，使所述收集物与具有多个能够与具有某些氨基酸的蛋白质结合的结合位点的部分接触 酸序列，以便形成部分 - 蛋白质复合物，并将复合物与足以表明复合物存在的量的该部分特异性标记物混合
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公开(公告)号：US06485943B2
公开(公告)日：2002-11-26
申请号：US09274163
申请日：1999-03-22
IPC分类号： C12N1500
CPC分类号： C07K16/00
摘要： A method for recombinant antibody subunit dimerization including modifying at least one codon of a nucleic acid sequence to replace an amino acid occurring naturally in the antibody with a charged amino acid at a position in the interface segment of the light polypeptide variable region, the charged amino acid having a first polarity; and modifying at least one codon of the nucleic acid sequence to replace an amino acid occurring naturally in the antibody with a charged amino acid at a position in an interface segment of the heavy polypeptide variable region corresponding to a position in the light polypeptide variable region, the charged amino acid having a second polarity opposite the first polarity. Nucleic acid sequences which code for novel light chain proteins, the latter of which are used in conjunction with the inventive method, are also provided.
摘要翻译： 一种用于重组抗体亚单位二聚化的方法，包括修饰核酸序列的至少一个密码子以用光多肽可变区的界面片段中的位置上的带电荷氨基酸代替抗体天然存在的氨基酸， 具有第一极性的酸; 以及修饰所述核酸序列的至少一个密码子以用对应于所述轻多肽可变区中位置的所述重多肽可变区的界面片段中的带电荷氨基酸替代所述抗体中天然存在的氨基酸， 带电荷的氨基酸具有与第一极性相反的第二极性。 还提供了编码新型轻链蛋白的核酸序列，其后者与本发明的方法结合使用。
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公开(公告)号：US07485443B2
公开(公告)日：2009-02-03
申请号：US10197189
申请日：2002-07-17
申请人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd Smith
发明人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd Smith
IPC分类号： C12P19/34
CPC分类号： C12Q1/683 , C12Q2565/549 , C12Q2561/109
摘要： The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5′ nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列变异的系统，组合物和方法。 本发明涉及将核酸连接到固体支持物和修饰核酸的方法。 例如，在一些实施方案中，切割剂的5'核酸酶活性用于切割在固体支持物上形成的切割结构，裂解事件的发生表明存在特定的核酸序列。
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