公开(公告)号：US11982611B2

公开(公告)日：2024-05-14

申请号：US16495738

申请日：2018-03-20

Applicant: NANOCELLECT BIOMEDICAL, INC.

Inventor： Sunghwan Cho ,  Jose Manuel Morachis ,  Ivan Gagne ,  Rick Segil ,  William Arthur Alaynick ,  Zhe Mei ,  Sean Phillips ,  Chien-Chun Yang ,  Dongseob Yun ,  Michael Jerome Benchimol ,  Manna Doud ,  Nicholas Sullivan ,  Constance Ardila

IPC: B01L3/00 ,  B07C5/342 ,  G01N15/14 ,  G01N15/10 ,  G01N15/149

CPC classification number: G01N15/1425 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  B07C5/342 ,  G01N15/1484 ,  B01L2200/0652 ,  B01L2300/0819 ,  B01L2400/082 ,  G01N2015/1006 ,  G01N15/149

Abstract: A method includes providing a cartridge and the cartridge includes a slot for receiving a microfluidic chip having a set of first channels. The cartridge also includes a set of second channels and each channel of the set of second channels is coupleable to a different channel of the set of first channels during use with the microfluidic chip. The cartridge also includes an indent configured for engagement and alignment of the cartridge during use. The method also includes inserting the cartridge into a device, such that the cartridge engages a first biasing member of the device configured for alignment of the cartridge in a first direction. The first biasing member is configured to bias movement of the cartridge into locking position with a notch of the device.

公开(公告)号：US20180163713A1

公开(公告)日：2018-06-14

申请号：US15738198

申请日：2016-06-23

Applicant: NanoCellect Biomedical, Inc.

Inventor： Jose M. MORACHIS ,  Sung Hwan CHO ,  Zhe MEI ,  Phillip POONKA ,  Constance ARDILA ,  Gerardo NAREZ ,  William ALAYNICK

IPC: F04B43/12 ,  B81B3/00 ,  F04B49/06 ,  F04B49/08 ,  F04B11/00 ,  F04B43/00

CPC classification number: F04B43/1292 ,  B81B3/00 ,  F04B11/0016 ,  F04B43/0081 ,  F04B43/043 ,  F04B43/12 ,  F04B49/06 ,  F04B49/08 ,  F04B2205/503

Abstract: A system includes a sorting chamber and a fluid channel in fluid communication with the sorting chamber. A peristaltic pump is in fluid communication with the fluid channel to pump fluid through the fluid channel to the sorting chamber at a fluid flow rate. A fluid damper is in fluid communication with the sample fluid channel. The fluid damper includes a gas and reduces variations in the fluid flow rate by compression and expansion of the gas in response to fluid flow in the fluid channel.

公开(公告)号：US20240344960A1

公开(公告)日：2024-10-17

申请号：US18624379

申请日：2024-04-02

Applicant: NanoCellect Biomedical, Inc.

Inventor： Sunghwan CHO ,  Jose Manuel MORACHIS ,  Ivan GAGNE ,  Rick SEGIL ,  William Arthur ALAYNICK ,  Zhe MEI ,  Sean PHILLIPS ,  Chien-Chun YANG ,  Dongseob YUN ,  Michael Jerome BENCHIMOL ,  Manna DOUD ,  Nicholas SULLIVAN ,  Constance ARDILA

IPC: G01N15/14 ,  B01L3/00 ,  B07C5/342 ,  G01N15/10 ,  G01N15/149

CPC classification number: G01N15/1425 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  B07C5/342 ,  G01N15/1484 ,  B01L2200/0652 ,  B01L2300/0819 ,  B01L2400/082 ,  G01N2015/1006 ,  G01N15/149

Abstract: A method includes providing a cartridge and the cartridge includes a slot for receiving a microfluidic chip having a set of first channels. The cartridge also includes a set of second channels and each channel of the set of second channels is coupleable to a different channel of the set of first channels during use with the microfluidic chip. The cartridge also includes an indent configured for engagement and alignment of the cartridge during use. The method also includes inserting the cartridge into a device, such that the cartridge engages a first biasing member of the device configured for alignment of the cartridge in a first direction. The first biasing member is configured to bias movement of the cartridge into locking position with a notch of the device.

公开(公告)号：US20230234056A1

公开(公告)日：2023-07-27

申请号：US18002019

申请日：2021-06-17

Applicant: NanoCellect Biomedical, Inc.

Inventor： Sung Hwan CHO ,  Ashley LO ,  Jason HANCOCK ,  Jose MORACHIS

IPC: B01L3/00 ,  B32B27/32 ,  B32B27/08 ,  B32B3/26 ,  B32B27/16 ,  B32B7/12 ,  B32B3/30

CPC classification number: B01L3/502761 ,  B01L3/502707 ,  B32B27/325 ,  B32B27/08 ,  B32B3/266 ,  B32B27/16 ,  B32B7/12 ,  B32B3/30 ,  B01L2200/12 ,  B01L2200/0652 ,  B01L2400/0439 ,  B01L2300/021 ,  B01L2200/027 ,  B01L2300/165 ,  B01L2300/168 ,  B01L2200/04 ,  B32B2307/412 ,  B32B2255/10 ,  B32B2457/08

Abstract: Provided herein are devices, systems, and methods for particle sorting, including cell sorting, using microfluidics cartridges and microchips and the manufacture of the microfluidics cartridges and microchips by high-throughput approaches. Such methods, devices, and systems can be used to identify, sort, and collect a subset of particles or a single particle from a sample. The capability to manufacture such microfluidic tools in high volume may lower production costs and allow for the microfluidic tools to be used as consumables.

公开(公告)号：US10816550B2

公开(公告)日：2020-10-27

申请号：US14435710

申请日：2013-10-15

Applicant: NANOCELLECT BIOMEDICAL, INC. ,  The Regents of the University of California

Inventor： Sung Hwan Cho ,  Jose Morachis ,  Yuhwa Lo ,  Tsung-Feng Wu ,  Ian Quigley ,  Kendall Chuang ,  Phillip Poonka ,  William Alaynick

IPC: G01N33/574 ,  G01N15/14 ,  G01N33/58 ,  B01L3/00 ,  G01N21/64 ,  G01N15/10

Abstract: Provided here are cell detection systems, fluidic devices, structures and techniques related to particle and cell sorting and detection in fluid, for example sorting specific subpopulations of cell types. A method for verification of sorting of particles includes receiving a first detection signal that is associated with optical characteristics of a particle in a first channel. A sorting channel of a plurality of second channels is determined based on the first detection signal, thereby determining the sorting of the particle into the sorting channel based on the optical characteristics of the particle. A sorting signal for sorting the particle from the first channel into the sorting channel is transmitted. A second detection signal is received that is associated with the presence of the particle in the sorting channel. The sorting of the particle from the first channel into the sorting channel is verified based on the second detection signal.

公开(公告)号：US20150268244A1

公开(公告)日：2015-09-24

申请号：US14435710

申请日：2013-10-15

Applicant: NANOCELLECT BIOMEDICAL, INC.

Inventor： Sung Hwan Cho ,  Jose Morachis ,  Yuhwa Lo ,  Tsung-Fend Wu ,  Ian Quigley ,  Kendall Chaung ,  Phillip Poonka ,  William Alaynick

IPC: G01N33/574 ,  G01N21/64 ,  B01L3/00 ,  G01N15/14

Abstract: Provided here are cell detection systems, fluidic devices, structures and techniques related to particle and cell sorting and detection in fluid, for example sorting specific subpopulations of cell types. A method for verification of sorting of particles includes receiving a first detection signal that is associated with optical characteristics of a particle in a first channel. A sorting channel of a plurality of second channels is determined based on the first detection signal, thereby determining the sorting of the particle into the sorting channel based on the optical characteristics of the particle. A sorting signal for sorting the particle from the first channel into the sorting channel is transmitted. A second detection signal is received that is associated with the presence of the particle in the sorting channel. The sorting of the particle from the first channel into the sorting channel is verified based on the second detection signal.

Abstract translation: 这里提供了细胞检测系统，流体装置，与流体中的颗粒和细胞分选和检测有关的结构和技术，例如分选细胞类型的特定亚群。 用于验证颗粒排序的方法包括接收与第一通道中的颗粒的光学特性相关联的第一检测信号。 基于第一检测信号确定多个第二通道的分选通道，从而基于颗粒的光学特性确定颗粒进入分选通道的分类。 发送用于将粒子从第一通道分类到排序通道的排序信号。 接收与分选通道中的粒子的存在相关联的第二检测信号。 基于第二检测信号，验证从第一通道到分选通道的粒子的分类。