公开(公告)号：US06316607B1

公开(公告)日：2001-11-13

申请号：US08472425

申请日：1995-06-07

申请人： Richard J. Massey ,  Michael J. Powell ,  Paul A. Mied ,  Peter Feng ,  Leopoldo Della Ciana ,  Walter J. Dressick ,  Mohindar S. Poonian

发明人： Richard J. Massey ,  Michael J. Powell ,  Paul A. Mied ,  Peter Feng ,  Leopoldo Della Ciana ,  Walter J. Dressick ,  Mohindar S. Poonian

IPC分类号： C12Q168

CPC分类号： G01N33/76 ,  C07D473/08 ,  C07F15/0026 ,  C07F15/0053 ,  C07H21/00 ,  C12Q1/04 ,  G01N33/533 ,  G01N33/569 ,  G01N33/582

摘要： Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids are provided. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety and capable of combining with the analyte of interest, exposing the resulting sample to electrochemical energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.

摘要翻译： 提供了对多组分液体中存在感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与标示有电化学发光化学部分的试剂接触，并能够与感兴趣的分析物结合，将所得样品暴露于电化学能并检测由电化学发光化学部分发射的电磁辐射。

公开(公告)号：US5811236A

公开(公告)日：1998-09-22

申请号：US468524

申请日：1995-06-06

申请人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo Della Ciana

发明人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo Della Ciana

IPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/00 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/569 ,  G01N33/58 ,  G01N33/76 ,  C07H21/04 ,  C07K15/28 ,  G01N23/53

CPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/0026 ,  C07F15/0053 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/6816 ,  G01N33/533 ,  G01N33/569 ,  G01N33/582 ,  G01N33/76 ,  G01N2458/30 ,  Y10S435/81

摘要： Electrochemiluminiscent moieties having the formula �Re(P).sub.m (L.sup.1).sub.n (L.sup.2).sub.o (L.sup.3).sub.p (L.sup.4).sub.q (L.sup.5).sub.r (L.sup.6).sub.s !.sub.t (B).sub.u wherein P is a polydentate ligand of Re; L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 and L.sup.6 are ligands of Re, each of which may be the same as or different from each other ligand; B is a substance which is a ligand of Re or is conjugated to one or more of P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 or L.sup.6 ; m is an integer equal to or greater than 1; each of n, o, p, q, r and s is zero or an integer; t is an integer equal to or greater than 1; and u is an integer equal to or greater than 1; P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5, L.sup.6 and B being of such composition and number that the chemical moiety can be induced to emit electromagnetic radiation and the total number of bonds to Re provided by the ligands of Re being equal to the coordination of Re are disclosed. Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids using these moieties are disclosed. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety containing rhenium and capable of combining with the analyte of interest, exposing the resulting sample to chemical, electrochemical, or electromagnetic energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.

摘要翻译： 具有式[Re（P）m（L1）n（L2）o（L3）p（L4）q（L5）r（L6）s] t（B）u的电化学发光部分，其中P是Re的多齿配体; L1，L2，L3，L4，L5和L6是Re的配体，其各自可以彼此相同或不同; B是作为Re的配体或与P，L1，L2，L3，L4，L5或L6中的一个或多个相结合的物质; m是等于或大于1的整数; n，o，p，q，r和s中的每一个为零或整数; t是等于或大于1的整数; u是等于或大于1的整数; P，L1，L2，L3，L4，L5，L6和B具有这样的组成和数量，可以诱导化学部分发射电磁辐射，并且由Re的配体提供的Re的总数相等于 Re的协调被披露。 公开了使用这些部分存在于多组分液体中的感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与含有铼的电化学发光化学部分标记的试剂接触并能够与感兴趣的分析物结合，将所得样品暴露于化学，电化学或电磁能并检测由电化学发光化学部分发射的电磁辐射。

公开(公告)号：US5716781A

公开(公告)日：1998-02-10

申请号：US470247

申请日：1995-06-06

申请人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo Della Ciana

发明人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo Della Ciana

IPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/00 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/569 ,  G01N33/58 ,  G01N33/76 ,  C12Q1/00 ,  C12Q1/70 ,  G01N33/48

CPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/0026 ,  C07F15/0053 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/6816 ,  G01N33/533 ,  G01N33/569 ,  G01N33/582 ,  G01N33/76 ,  G01N2458/30 ,  Y10S435/81

摘要： Electrochemiluminiscent moieties having the formula �Re(P).sub.m (L.sup.1).sub.n (L.sup.2).sub.o (L.sup.3).sub.p (L.sup.4).sub.q (L.sup.5).sub.r (L.sup.6).sub.s !.sub.t (B).sub.u wherein P is a polydentate ligand of Re; L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 and L.sup.6 are ligands of Re, each of which may be the same as or different from each other ligand; B is a substance which is a ligand of Re or is conjugated to one or more of P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 or L.sup.6 ; m is an integer equal to or greater than 1; each of n, o, p, q, r and s is zero or an integer; t is an integer equal to or greater than 1; and u is an integer equal to or greater than 1; P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5, L.sup.6 and B being of such composition and number that the chemical moiety can be induced to emit electromagnetic radiation and the total number of bonds to Re provided by the ligands of Re being equal to the coordination of Re are disclosed. Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids using these moieties are disclosed. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety containing rhenium and capable of combining with the analyte of interest, exposing the resulting sample to chemical, electrochemical, or electromagnetic energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.

摘要翻译： 具有式[Re（P）m（L1）n（L2）o（L3）p（L4）q（L5）r（L6）s] t（B）u的电化学发光部分其中P是Re的多齿配体; L1，L2，L3，L4，L5和L6是Re的配体，其各自可以彼此相同或不同; B是作为Re的配体或与P，L1，L2，L3，L4，L5或L6中的一个或多个相结合的物质; m是等于或大于1的整数; n，o，p，q，r和s中的每一个为零或整数; t是等于或大于1的整数; u是等于或大于1的整数; P，L1，L2，L3，L4，L5，L6和B具有这样的组成和数量，可以诱导化学部分发射电磁辐射，并且由Re的配体提供的Re的总数相等于 Re的协调被披露。 公开了使用这些部分存在于多组分液体中的感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与含有铼的电化学发光化学部分标记的试剂接触并能够与感兴趣的分析物结合，将所得样品暴露于化学，电化学或电磁能并检测由电化学发光化学部分发射的电磁辐射。

公开(公告)号：US06916606B2

公开(公告)日：2005-07-12

申请号：US10274079

申请日：2002-10-18

申请人： Richard J. Massey ,  Michael J. Powell ,  Paul A. Mied ,  Peter Feng ,  Leopoldo Della Ciana ,  Walter J. Dressick ,  Mohindar S. Poonian

发明人： Richard J. Massey ,  Michael J. Powell ,  Paul A. Mied ,  Peter Feng ,  Leopoldo Della Ciana ,  Walter J. Dressick ,  Mohindar S. Poonian

IPC分类号： C07D473/08 ,  C07F15/00 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/569 ,  G01N33/58 ,  G01N33/76 ,  C12Q1/70 ,  A61K38/00 ,  G01N33/53 ,  G01N33/566

CPC分类号： G01N33/76 ,  C07D473/08 ,  C07F15/0026 ,  C07F15/0053 ,  C07H21/00 ,  C12Q1/04 ,  G01N33/533 ,  G01N33/569 ,  G01N33/582

摘要： Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids are provided. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety and capable of combining with the analyte of interest, exposing the resulting sample to electrochemical energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.Further provided are methods for detecting and identifying the presence of a multiplicity of analytes in a liquid food or food homogenate. These methods comprise immersing a diagnostic reagent holder, provided with a multiplicity of reagents, into the food or food homogenate, removing the diagnostic reagent holder from the liquid food or food homogenate, and detecting and identifying the presence of a multiplicity of analytes of interest bound to the diagnostic reagent holder, thereby detecting and identifying the presence of a multiplicity of analytes of interest in the food or food homogenate.The invention further provides an enzyme immunoassay for coliform bacteria. This assay comprises inoculating a sample into a suitable medium for coliform reproduction, immobilizing coliforms present in the medium to a suitable surface, treating the surface with an antibody directed to the immobilized coliforms and detecting the presence of the immobilized coliforms immobilized to a suitable surface.

摘要翻译： 提供了对多组分液体中存在感兴趣分析物的定性和定量的电化学发光测定。 这些方法包括使样品与标示有电化学发光化学部分的试剂接触，并能够与感兴趣的分析物结合，将所得样品暴露于电化学能并检测由电化学发光化学部分发射的电磁辐射。 还提供了用于检测和鉴定液体食物或食物匀浆中多种分析物的存在的方法。 这些方法包括将设置有多种试剂的诊断试剂保持器浸入食物或食物匀浆中，从液体食物或食物匀浆中除去诊断试剂载体，以及检测和鉴定多种感兴趣分析物的存在 到诊断试剂保持器，从而检测和鉴定食物或食物匀浆中感兴趣的多种分析物的存在。 本发明还提供了大肠杆菌的酶免疫测定法。 该测定包括将样品接种到用于大肠杆菌繁殖的合适的培养基中，将存在于培养基中的大肠杆菌固定在合适的表面上，用针对固定的大肠杆菌的抗体处理该表面并检测固定在合适的表面上的固定的大肠杆菌的存在。

公开(公告)号：US06468741B1

公开(公告)日：2002-10-22

申请号：US09157788

申请日：1998-09-21

申请人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo Della Ciana

发明人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo Della Ciana

IPC分类号： C12Q168

CPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/0026 ,  C07F15/0053 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/6816 ,  G01N33/533 ,  G01N33/569 ,  G01N33/582 ,  G01N33/76 ,  G01N2458/30 ,  Y10S435/81

摘要： A labeled substance comprising a biological substance linked to a luminescent rhenium-containing label. Qualitative and quantitative electrochemiluminescent assays using the same. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety containing rhenium and capable of combining with the analyte of interest, exposing the resulting sample to chemical, electrochemical, or electromagnetic energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.

摘要翻译： 包含与发光含铼标记物连接的生物物质的标记物质。 使用相同的定性和定量电化学发光测定。 这些方法包括使样品与含有铼的电化学发光化学部分标记的试剂接触并能够与感兴趣的分析物结合，将所得样品暴露于化学，电化学或电磁能并检测由电化学发光化学部分发射的电磁辐射。

公开(公告)号：US5591581A

公开(公告)日：1997-01-07

申请号：US227898

申请日：1994-04-15

申请人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo D. Ciana

发明人： Richard J. Massey ,  Michael J. Powell ,  Walter J. Dressick ,  Jonathan K. Leland ,  Janel K. Hino ,  Mohindar S. Poonian ,  Leopoldo D. Ciana

IPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/00 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/569 ,  G01N33/58 ,  G01N33/76

CPC分类号： C07D213/22 ,  C07D213/48 ,  C07D213/55 ,  C07D401/12 ,  C07D405/14 ,  C07D473/08 ,  C07F13/00 ,  C07F15/0026 ,  C07F15/0053 ,  C07H21/00 ,  C12Q1/04 ,  C12Q1/6816 ,  G01N33/533 ,  G01N33/569 ,  G01N33/582 ,  G01N33/76 ,  G01N2458/30 ,  Y10S435/81

摘要： Electrochemiluminescent moieties having the formula(Re(P).sub.m (L.sup.1).sub.n (L.sup.2).sub.o (L.sup.3).sub.p (L.sup.4).sub.t (B).sub.uwhereinP is a polydentate ligand of Re;L.sup.1, L.sup.2, L.sup.3 and L.sup.4 are ligands of Re, each of which may be the same as or not the same as each other ligand;B is a substance which is a ligand of Re or is conjugated to one or more of P, L.sup.1, L.sup.2, L.sup.3 and L.sup.4 ;m is an integer equal to or greater than 1;each of n, o, p, q, r and s is zero or an integer;t is an integer equal to or greater than 1; andu is an integer equal to or greater than 1;P, L.sup.1, L.sup.2, L.sup.3, L.sup.4 and B being of such composition and number that the chemical moiety can be induced to electrochemiluminesce and the total number of bonds to Re provided by the ligands of Re being equal to the coordination number of Re are disclosed.Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids using these moieties are also disclosed.

摘要翻译： 具有式（Re（P）m（L1）n（L2）o（L3）p（L4）t（B）u的电化学发光部分，其中P是Re; L1，L2，L3和L4的多齿配体， Re，其各自可以与每个其它配体相同或不相同; B是作为Re的配体或与P，L1，L2，L3和L4中的一个或多个相结合的物质; m是 整数等于或大于1; n，o，p，q，r和s中的每一个为零或整数; t为等于或大于1的整数; u为等于或大于1的整数; P，L1，L2，L3，L4和B具有化学部分可以被诱导为电化学发光的组成和数量，并且Re的配体提供的Re的总数等于Re的配位数 还公开了使用这些部分存在于多组分液体中的感兴趣分析物的定性和定量电化学发光测定。

公开(公告)号：US4728609A

公开(公告)日：1988-03-01

申请号：US778779

申请日：1985-09-24

申请人： Ram S. Bhatt ,  Kenneth J. Collier ,  Robert M. Crowl ,  Mohindar S. Poonian

发明人： Ram S. Bhatt ,  Kenneth J. Collier ,  Robert M. Crowl ,  Mohindar S. Poonian

IPC分类号： C07H21/00 ,  C12P21/00 ,  C07H15/12 ,  C12N1/00 ,  C12N1/20 ,  C12N15/00 ,  C12P21/02

CPC分类号： C07H21/00 ,  Y10S930/12

摘要： Th preparation of a double-stranded polydeoxy ribonucleotide having cohesive termini each comprising one strand of a double-strand restriction endonuclease recognition site and, between the termini, a structural gene coating for the expression of recombinant growth hormone releasing factor, at least a majority of the codons and the coding strand of said gene being codons preferred for the expression of microbial genomes; a method of producing such DNA a recombinant microbial cloning vehicle comprising such DNA and the novel methods of producing said peptide are described.

摘要翻译： 制备具有粘连末端的双链多脱氧核糖核苷酸，每个包含双链限制性内切核酸酶识别位点的一条链，并且在该末端之间，用于表达重组生长激素释放因子的结构基因包膜，至少大部分 所述基因的密码子和编码链是用于表达微生物基因组的密码子; 描述了生产这种DNA的方法，包含这种DNA的重组微生物克隆载体和产生所述肽的新方法。