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公开(公告)号：US07811775B2
公开(公告)日：2010-10-12
申请号：US12127350
申请日：2008-05-27
IPC分类号： G01N33/53
CPC分类号： G01N33/542 , G01N33/582
摘要： A homogeneous assay for determining the aflatoxin content in agricultural products uses the technique of fluorescence polarization. A solvent is used to extract aflatoxins from a sample of the agricultural product. A mixture is prepared by combining the extract with a tracer and with a monoclonal antibody specific for aflatoxin. The tracer is able to bind to the monoclonal antibody to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating an aflatoxin oxime to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The aflatoxin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of aflatoxin solutions of known concentration.
摘要翻译： 用于确定农产品中黄曲霉毒素含量的均匀测定法使用荧光偏振技术。 使用溶剂从农产品样品中提取黄曲霉毒素。 通过将提取物与示踪剂和特异于黄曲霉毒素的单克隆抗体组合来制备混合物。 示踪剂能够结合单克隆抗体以产生可检测的荧光偏振变化。 通过将黄曲霉毒素与合适的荧光团缀合来制备示踪剂。 测量混合物的荧光偏振。 可以使用通过测量已知浓度的一系列黄曲霉毒素溶液的荧光偏振获得的标准曲线来计算混合物的黄曲霉毒素浓度。
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2.发明授权公开(公告)号：US06596546B1
公开(公告)日：2003-07-22
申请号：US09400798
申请日：1999-09-22
IPC分类号： G01N33533
CPC分类号： G01N33/56916 , G01N33/56911 , G01N33/5695 , G01N2333/23 , G01N2333/35 , G01N2400/50 , Y02A50/56
摘要： In a homogeneous immunoassay, fluorophore-conjugated lipopolysaccharide derived bacterial antigens are reacted with antibodies specific for the antigens. Quantitative detection of the formation of an immune complex is obtained by measuring the change in fluorescence polarization after complex formation. The reaction occurs quickly (less than two minutes to equilibrium), and involves the addition of only one reagent to a diluted serum specimen. The absence of a solid phase separation step eliminates false positive results and increases throughput.
摘要翻译： 在均相免疫测定中，使荧光团结合的脂多糖衍生的细菌抗原与抗原特异性抗体反应。 通过测量复合物形成后荧光极化的变化，可以获得免疫复合物形成的定量检测。 反应快速发生（不到两分钟至平衡），并且仅向稀释的血清样品中加入一种试剂。 没有固相分离步骤消除了假阳性结果并增加了产量。
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3.发明授权Fluorescent polarization immunoassay utilizing substituted triazinylaminofluoresceins 失效荧光偏振免疫测定利用取代的三嗪基氨基荧光素
公开(公告)号：US4420568A
公开(公告)日：1983-12-13
申请号：US325872
申请日：1981-11-30
IPC分类号： C07D473/08 , C07H15/236 , G01N33/533 , G01N33/54 , G01N33/52 , G01N33/58 , G01N33/74
CPC分类号： C07H15/234 , C07D473/08 , G01N33/533 , Y10S436/80 , Y10S436/817
摘要： An immunochemical method and reagent compounds for determining ligands in a sample. The reagent compounds are derivatives of a triazinylaminofluorescein and are represented by the structural formula ##STR1## wherein Y is halo or lower alkyl; andR is a ligand-analog wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody.The reagent compound and an antibody specific to the ligand are added to the sample in a fluorescence polarization immunoassay.
摘要翻译： 用于测定样品中配体的免疫化学方法和试剂化合物。 试剂化合物是三嗪基氨基荧光素的衍生物，其结构式为：其中Y为卤素或低级烷基; 并且R是配体 - 类似物，其中所述配体 - 类似物与所述配体具有至少一个共同表位，以便被共同抗体特异性地识别。 将试剂化合物和对配体特异的抗体以荧光偏振免疫测定法加入到样品中。
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公开(公告)号：US4131798A
公开(公告)日：1978-12-26
申请号：US784900
申请日：1977-04-05
摘要： A radioactive particle counter for a plurality of discrete radioactive particle emitting samples, including a plurality of detectors for each sample to simultaneously detect the respective emitted radioactive particles and provide a respective electrical signal, a plurality of storage counters for simultaneously accumulating the respective sample count during a predetermined counting interval, and a read-out control unit for periodically, sequentially reading out the accumulated sample count in each storage counter and coupling same to a control counter for temporary display of the accumulated sample count or for eventually totalling of the respective sample count.
摘要翻译： 一种用于多个离散放射性粒子发射样品的放射性粒子计数器，包括用于每个样本的多个检测器，用于同时检测各个发射的放射性粒子并提供相应的电信号;多个存储计数器，用于在 预定的计数间隔和读出控制单元，用于周期性地顺序地读出每个存储计数器中累积的采样计数并将其耦合到控制计数器，以暂时显示累积的采样计数或最终合计各个采样计数 。
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5.发明授权Fluorescence polarization—based diagnostic assay for equine infectious anemia virus 有权基于荧光偏振的马传染性贫血病毒诊断试验公开(公告)号：US06350574B1
公开(公告)日：2002-02-26
申请号：US09400564
申请日：1999-09-21
IPC分类号： C12Q170
CPC分类号： G01N33/533 , G01N33/56983 , G01N2333/155 , Y10S435/968
摘要： A fluorescence polarization assay for Equine Infectious Anemia Virus utilizes a short peptide reagent probe derived from a conserved immunodominant region of gp45. The probe is N-terminally labeled, preferably with 6-carboxyfluorescein, and purified by HPLC, which reacts in a homogenous assay with anti-EIAV antibodies contained in the serum of field infected horses and ponies. The assay has a sensitivity of about 90 percent with a specificity approaching 100 percent.
摘要翻译： 马传染性贫血病毒的荧光偏振测定利用源自gp45的保守免疫显性区域的短肽试剂探针。 探针是N-末端标记的，优选用6-羧基荧光素，并通过HPLC纯化，其在均匀测定中与包含在场感染的马和小马血清中的抗EIAV抗体反应。 该测定具有约90％的灵敏度，特异性接近100％。
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公开(公告)号：US5097097A
公开(公告)日：1992-03-17
申请号：US376190
申请日：1989-06-30
IPC分类号： C07D473/08 , C07H15/236 , C07H21/00 , G01N33/533
CPC分类号： C07H15/234 , C07D473/08 , C07H21/00 , G01N33/533
摘要： This disclosure relates to a method and reagents for determining ligands in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine. In particular, this disclosure relates to a fluorescent polarization immunoassay procedure and to a novel class of tracer compounds employed as reagents in such procedures. The procedure disclosed combines the specificity of an immunoassay with the speed and convenience of fluorescent polarization techniques to provide a means for determining the amount of a specific ligand present in a sample.
摘要翻译： 本公开涉及用于测定生物流体如血清，血浆，脊髓液，羊膜液和尿液中配体的方法和试剂。 特别地，本公开内容涉及荧光偏振免疫测定方法以及在这种方法中用作试剂的新型示踪剂化合物。 所公开的方法将免疫测定的特异性与荧光偏振技术的速度和方便相结合，以提供测定样品中存在的特定配体的量的方法。
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7.发明授权Fluorescence polarization immunoassay utilizing substituted carboxyfluoresceins 失效荧光偏振免疫测定利用取代的羧基荧光素
公开(公告)号：US4668640A
公开(公告)日：1987-05-26
申请号：US730233
申请日：1985-05-02
IPC分类号： C07D311/82 , C07D405/12 , C07D473/08 , C07J41/00 , G01N33/533 , G01N33/58 , G01N33/536
CPC分类号： C07D405/12 , C07D311/82 , C07D473/08 , C07J41/0005 , G01N33/533 , G01N33/582 , Y10S436/80
摘要： This disclosure relates to a method and reagents for determining ligands in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure and to a novel class of tracer compounds employed as reagents in such procedures. The procedure disclosed combines the specificity of an immunoassay with the speed and convenience of fluorescence polarization techniques to provide a means for determining the amount of a specific ligand present in a sample.
摘要翻译： 本公开涉及用于测定生物流体如血清，血浆，脊髓液，羊膜液和尿液中配体的方法和试剂。 特别地，本发明涉及荧光偏振免疫测定方法以及在这种方法中用作试剂的新型示踪剂化合物。 所公开的方法将免疫测定的特异性与荧光偏振技术的速度和方便相结合，以提供测定样品中存在的特定配体的量的方法。
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8.发明授权Fluorescent polarization immunoassay utilizing substituted triazinylaminofluorescein aminoglycosides 失效荧光偏振免疫测定利用取代的三嗪基氨基荧光素氨基糖苷类
公开(公告)号：US4593089A
公开(公告)日：1986-06-03
申请号：US546778
申请日：1983-10-31
IPC分类号： C07D473/08 , C07H15/236 , C07H21/00 , G01N33/533 , C07H15/232 , C07H15/234
CPC分类号： C07H15/234 , C07D473/08 , C07H21/00 , G01N33/533
摘要： This disclosure relates to a method and reagents for determining ligands in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine. In particular, this disclosure relates to a fluorescent polarization immunoassay procedure and to a novel class of tracer compounds employed as reagents in such procedures. The procedure disclosed combines the specificity of an immunoassay with the speed and convenience of fluorescent polarization techniques to provide a means for determining the amount of a specific ligand present in a sample.
摘要翻译： 本公开涉及用于测定生物流体如血清，血浆，脊髓液，羊膜液和尿液中配体的方法和试剂。 特别地，本公开内容涉及荧光偏振免疫测定方法以及在这种方法中用作试剂的新型示踪剂化合物。 所公开的方法将免疫测定的特异性与荧光偏振技术的速度和方便相结合，以提供测定样品中存在的特定配体的量的方法。
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9.发明授权Peptide-based fluorescence polarization assay for detection of antibodies to Mycobacterium bovis 有权用于检测牛分枝杆菌抗体的基于肽的荧光偏振测定法公开(公告)号：US07547506B2
公开(公告)日：2009-06-16
申请号：US10492998
申请日：2002-10-31
IPC分类号： C12Q1/00 , A61K39/04 , G01N33/566 , A61K38/00
CPC分类号： G01N33/5695 , C07K14/35 , G01N33/542
摘要： The present invention provides an assay for detection of M. bovis-infected animals. A tracer, comprising a peptide of M. bovis protein MPB70 conjugated to a fluorophore, is added to a serum sample from an animal to form a mixture. The fluorescence polarization of the mixture in then measured and compared to the fluorescence polarization of a control. The present invention further provides a tracer for use in fluorescence polarization assay to detect antibodies specific for M. bovis. The tracer comprises a peptide of M. bovis protein MPB70 conjugated to a fluorophore, such that the tracer is able to bind to antibodies specific for M. bovis to produce a detectable change in fluorescence polarization.
摘要翻译： 本发明提供了用于检测牛分枝杆菌感染动物的检测方法。 将包含与荧光团缀合的牛分枝杆菌蛋白MPB70的肽的示踪剂加入到来自动物的血清样品中以形成混合物。 然后测量混合物的荧光偏振，并与对照的荧光极化进行比较。 本发明还提供了用于荧光偏振测定以检测牛分枝杆菌特异性的抗体的示踪剂。 示踪剂包含与荧光团缀合的牛分枝杆菌蛋白MPB70的肽，使得示踪剂能够结合对牛分枝杆菌特异性的抗体以产生可检测的荧光偏振变化。
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10.发明授权Fluorescence polarization-based homogeneous assay for fumonisin determination in grains 有权基于荧光偏振的均相测定法测定米色霉菌公开(公告)号：US06482601B1
公开(公告)日：2002-11-19
申请号：US09659663
申请日：2000-09-11
IPC分类号： G01N33569
CPC分类号： G01N33/542 , G01N21/6445 , G01N33/56961 , Y10S435/81 , Y10S435/975 , Y10S436/825 , Y10S530/823 , Y10T436/107497 , Y10T436/25375
摘要： A homogeneous assay for determining the fumonisin content in grains uses the technique of fluorescence polarization. A grain extract is prepared by shaking a crushed grain sample with a solvent. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to fumonisin. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating fumonisin to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The fumonisin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of fumonisin solutions of known concentration.
摘要翻译： 用于测定谷粒中伏马毒素含量的均匀测定使用荧光偏振技术。 通过用溶剂摇动破碎的谷物样品来制备谷物提取物。 通过将谷物提取物与示踪剂和特异于伏马菌素的单克隆抗体组合来制备混合物。 示踪剂能够结合单克隆抗体以产生可检测的荧光偏振变化。 通过将伏马毒素与合适的荧光团缀合来制备示踪剂。 测量混合物的荧光偏振。 可以使用通过测量已知浓度的一系列伏马菌素溶液的荧光偏振获得的标准曲线来计算混合物的伏马毒素浓度。
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