公开(公告)号：US06433144B1

公开(公告)日：2002-08-13

申请号：US09229039

申请日：1999-01-12

Applicant: Joseph P. Morris ,  Duy Nguyen ,  James Kappelman ,  Michael D. Potter ,  Mead M. McCabe ,  Reza Ziai ,  Stephen Feldman ,  Hipolito Hartman

Inventor： Joseph P. Morris ,  Duy Nguyen ,  James Kappelman ,  Michael D. Potter ,  Mead M. McCabe ,  Reza Ziai ,  Stephen Feldman ,  Hipolito Hartman

IPC: C07K1700

CPC classification number: C07K14/56 ,  A61K38/00

Abstract: This invention relates to methods for isolating highly purified mixtures of natural Type I interferon from white blood cells. The invention also relates to highly-purified mixtures of Type I interferon which resemble natural Type I interferon in that the highly purified mixtures of natural Type I interferon includes at least 9 subtypes, i.e., alpha-1, alpha-2, alpha-5, alpha-7, alpha-8, alpha-10, alpha-14, alpha-21 and omega, giving rise to at least 16, and possibly 20 or more molecular species, including alpha-1a, alpha-1new, alpha-2a, alpha-2b, alpha-2c, alpha-5, alpha-5LG, alpha-7, alpha-8a, alpha-8c, alpha-10a, alpha-14a, alphal4-b, alpha 14-c, alpha-14LG, alpha-21a, alpha-21b, alpha-21c, omega and omega LG.

Abstract translation: 本发明涉及从白细胞中分离出高度纯化的天然I型干扰素混合物的方法。 本发明还涉及类似于天然I型干扰素的I型干扰素的高度纯化的混合物，因为天然I型干扰素的高度纯化的混合物包括至少9种亚型，即α-1，α-2，α-5， α-7，α-8，α-10，α-14，α-21和ω，产生至少16种，并且可能有20种或更多种分子物质，包括α-1a，α-1新，α-2a， α-2b，α-2c，α-5，α-5LG，α-7，α-8a，α-8c，α-10a，α-14a，alphal4-b，α14-c，α-14LG， -21a，α-21b，α-21c，ω和ωLG。

公开(公告)号：US5593829A

公开(公告)日：1997-01-14

申请号：US241385

申请日：1994-05-03

Applicant: Mead M. McCabe

Inventor： Mead M. McCabe

IPC: C07H15/12 ,  C07H19/04 ,  C07H19/06 ,  C07H19/16 ,  C07H21/00 ,  C12Q1/68 ,  C07H21/04 ,  C07H19/167 ,  G01N33/53

CPC classification number: C07H15/12 ,  C07H19/04 ,  C07H19/06 ,  C07H19/16 ,  C07H21/00 ,  C12Q1/6816 ,  Y10S435/975

Abstract: The invention provides methods for attaching detectable labels to the N.sup.7 -formyl group, of imidazole ring-opened alkylated purines and purine derivatives, directly, through a linking group, or by subsequent derivatization of a blocking group. The invention provides simple and efficient methods for labeling polynucleotides. In addition, the invention provides derivatized ring-opened alkylated purines and purine derivatives, including reagents for chemical and enzymatic synthesis of detectably labeled polynucleotides.

Abstract translation: 本发明提供了通过连接基团直接将可检测标记连接到咪唑开环烷基化嘌呤和嘌呤衍生物的N7-甲酰基，咪唑开环烷基化嘌呤和嘌呤衍生物的方法，或通过随后的封闭基团的衍生化。 本发明提供用于标记多核苷酸的简单而有效的方法。 此外，本发明提供衍生化的开环烷基化嘌呤和嘌呤衍生物，包括用于化学和酶合成可检测标记的多核苷酸的试剂。

公开(公告)号：US5656742A

公开(公告)日：1997-08-12

申请号：US469925

申请日：1995-06-06

Applicant: Mead M. McCabe

Inventor： Mead M. McCabe

IPC: C07H15/12 ,  C07H19/04 ,  C07H19/06 ,  C07H19/16 ,  C07H21/00 ,  C12Q1/68 ,  C07H21/04 ,  C07H19/073

CPC classification number: C07H15/12 ,  C07H19/04 ,  C07H19/06 ,  C07H19/16 ,  C07H21/00 ,  C12Q1/6816 ,  Y10S435/975

Abstract: The invention provides methods for attaching detectable labels to the N.sup.7 -formyl group, of imidazole ring-opened alkylated purines and purine derivatives, directly, through a linking group, or by subsequent derivatization of a blocking group. The invention provides simple and efficient methods for labeling polynucleotides. In addition, the invention provides derivatized ring-opened alkylated purines and purine derivatives, including reagents for chemical and enzymatic synthesis of detectably labeled polynucleotides.

Abstract translation: 本发明提供了通过连接基团直接将可检测标记连接到咪唑开环烷基化嘌呤和嘌呤衍生物的N7-甲酰基，咪唑开环烷基化嘌呤和嘌呤衍生物的方法，或通过随后的封闭基团的衍生化。 本发明提供用于标记多核苷酸的简单而有效的方法。 此外，本发明提供衍生化的开环烷基化嘌呤和嘌呤衍生物，包括用于化学和酶合成可检测标记的多核苷酸的试剂。

公开(公告)号：US06350589B1

公开(公告)日：2002-02-26

申请号：US09224895

申请日：1998-12-31

Applicant: Joseph P. Morris ,  Duy Nguyen ,  James Kappelman ,  Michael D. Potter ,  Mead M. McCabe ,  Reza Ziai ,  Stephen B. Feldman ,  Hipolito Hartman

Inventor： Joseph P. Morris ,  Duy Nguyen ,  James Kappelman ,  Michael D. Potter ,  Mead M. McCabe ,  Reza Ziai ,  Stephen B. Feldman ,  Hipolito Hartman

IPC: C12P100

CPC classification number: C07K14/56 ,  A61K38/00

Abstract: This invention relates to methods for isolating highly-purified mixtures of natural type I interferons from white blood cells. The invention also relates to highly-purified mixtures of natural type I interferons which resemble natural type I interferon in that it includes 9 subtypes, i.e., alpha-1, alpha-2, alpha-5, alpha-7, alpha-8, alpha-10, alpha-14, alpha-21 and omega, giving rise to possibly 20 molecular species, including alpha-1a, alpha-1new, alpha-2a, alpha-2b, alpha-2c, alpha-5, alpha-5LG, alpha-7, alpha-8a, alpha-8c, alpha-10a, alpha-14a, alpha14-b, alpha 14-c, alpha-14LG, alpha-21a, alpha-21b, alpha-21c, omega and omega LG.

Abstract translation: 本发明涉及从白细胞分离天然I型干扰素的高度纯化混合物的方法。 本发明还涉及类似于天然I型干扰素的天然I型干扰素的高度纯化的混合物，因为它包括9种亚型，即α-1，α-2，α-5，α-7，α-8，α -10，α-14，α-21和ω，产生可能20个分子物质，包括α-1a，α-1新，α-2a，α-2b，α-2c，α-5，α-5LG， α-7，α-8a，α-8c，α-10a，α-14a，α14-b，α14-c，α-14LG，α-21a，α-21b，α-21c，ω和ω-LG。