公开(公告)号：US08858959B2

公开(公告)日：2014-10-14

申请号：US13546455

申请日：2012-07-11

申请人： Mark C. Jenkins ,  Raymond H. Fetterer ,  Joseph T. Persyn

发明人： Mark C. Jenkins ,  Raymond H. Fetterer ,  Joseph T. Persyn

IPC分类号： A61K39/012 ,  A61K39/002 ,  A61K9/16 ,  A61K9/00 ,  A61K39/00

CPC分类号： A61K39/002 ,  A61K9/0056 ,  A61K9/1658 ,  A61K39/012 ,  A61K2039/54 ,  A61K2039/542

摘要： Safe and effective gel-bead vaccines for treating domesticated birds for diseases caused by cyst-forming protozoa, especially for coccidiosis.

摘要翻译： 安全有效的凝胶珠疫苗用于治疗家禽由于囊肿形成原生动物引起的疾病，特别是针对球虫病。

公开(公告)号：US20130017220A1

公开(公告)日：2013-01-17

申请号：US13546455

申请日：2012-07-11

申请人： Mark C. Jenkins ,  Raymond H. Fetterer ,  Joseph T. Persyn

发明人： Mark C. Jenkins ,  Raymond H. Fetterer ,  Joseph T. Persyn

IPC分类号： A61K39/002 ,  A61P33/02 ,  A61K39/012

CPC分类号： A61K39/002 ,  A61K9/0056 ,  A61K9/1658 ,  A61K39/012 ,  A61K2039/54 ,  A61K2039/542

摘要： Safe and effective gel-bead vaccines for treating domesticated birds for diseases caused by cyst-forming protozoa, especially for coccidiosis.

摘要翻译： 安全有效的凝胶珠疫苗用于治疗家禽由于囊肿形成原生动物引起的疾病，特别是针对球虫病。

公开(公告)号：US06202249B1

公开(公告)日：2001-03-20

申请号：US09226896

申请日：1999-01-07

申请人： Mark C. Jenkins ,  Jay M. Jenkins

发明人： Mark C. Jenkins ,  Jay M. Jenkins

IPC分类号： A47L1326

CPC分类号： A47L13/284 ,  A47L13/30

摘要： A floor coating application device with a rigid body having attached thereto a removable applicator and a removable weight system. The rigid body having a planar surface with radial edges to facilitate the uniform application of a coating material to a surface. The removable applicator being a material suitable for uniformly spreading a coating material without flaws or shedding and attached to the rigid body in such a manner as to minimize the wicking of coating material through the applicator. The removable weight system capable of providing a consistent force against the rigid body to improve the uniformity of coating thicknesses. The floor coating application device is used to spread coating material with a handle connected to the rigid body or a mechanical coating device.

摘要翻译： 一种具有刚性体的地板涂布施加装置，其附接有可拆卸的施加器和可拆卸的重量系统。 该刚体具有具有径向边缘的平坦表面，以便于将涂层材料均匀地涂覆到表面上。 可移除的涂抹器是适于均匀地涂覆涂料的材料，没有瑕疵或脱落并附着到刚体，使得通过涂布器的涂料的芯吸最小化。 可拆卸的重量系统能够提供一致的力抵抗刚体，以改善涂层厚度的均匀性。 地板涂布施加装置用于铺展涂层材料，手柄连接到刚体或机械涂层装置。

公开(公告)号：US5591434A

公开(公告)日：1997-01-07

申请号：US229393

申请日：1994-04-15

申请人： Mark C. Jenkins ,  Ronald Fayer ,  Michael Tilley ,  Steven L. Upton

发明人： Mark C. Jenkins ,  Ronald Fayer ,  Michael Tilley ,  Steven L. Upton

IPC分类号： A61K39/00 ,  C07K14/44 ,  C07K16/04 ,  C07K16/20 ,  A61K39/002 ,  A61K39/395 ,  C12N15/30 ,  C12N1/21 ,  C12P21/02 ,  C12P21/08

CPC分类号： C07K16/04 ,  C07K14/44 ,  C07K16/20 ,  A61K39/00

摘要： Recombinant proteins have been developed for the immunization of animals against cryptosporidiosis. The Proteins are effective for the immunization of a variety of animals against Cryptosporidium parvum, particularly for the production of hyperimmune colostrum that may be used to confer passive immunity against the parasite. Isolated DNA sequences which encode these proteins have also been developed. The DNA sequences may be inserted into recombinant DNA molecules such as cloning vectors or expression vectors for the transformation of cells and the production of the proteins.

摘要翻译： 已经开发了用于免疫动物以防止隐孢子虫病的重组蛋白质。 蛋白质可有效地免疫多种动物对小隐孢子虫，特别是用于生产可用于赋予对寄生虫的被动免疫的超免疫初乳。 已经开发了编码这些蛋白质的分离的DNA序列。 可以将DNA序列插入到重组DNA分子中，例如用于转化细胞和产生蛋白质的克隆载体或表达载体。

公开(公告)号：US5122471A

公开(公告)日：1992-06-16

申请号：US308219

申请日：1989-02-09

申请人： Mark C. Jenkins ,  Hyun S. Lillehoj ,  John B. Dame ,  Harry D. Danforth ,  Michael D. Ruff

发明人： Mark C. Jenkins ,  Hyun S. Lillehoj ,  John B. Dame ,  Harry D. Danforth ,  Michael D. Ruff

IPC分类号： A61K39/00 ,  C07K14/455 ,  C07K16/20 ,  C12N1/21

CPC分类号： C07K14/455 ,  C07K16/20 ,  A61K39/00

摘要： Disclosed are DnA sequences which code for antigenic proteins, methods for identifying such DNA sequences, and antigens coded for by such DNA sequences.The first step of the method is to provide a multiplicity of DNA sequences. These sequences are then inserted into DNA expression vectors to form recombinant expression vectors. The expression vectors are inserted into suitable hosts to form transformants which express the DNA sequences. The transformants are then contacted with antibodies directed against Eimeria antigens to identify transformants containing DNA sequences which code for Eimeria antigens. These antigens are then produced from the DNA sequences identified as coding for the antigens. The antigens so produced are contacted with white blood cells which effect a cell-mediated immune response, which white blood cells are sensitized to an antigenic Eimeria protein, to thereby identify DNA sequences which code for antigens that induce a cell-mediated immune response to avian coccidiosis.The DNA sequences of the present invention comprise cloned genes or fragments thereof that code on expression for an antigenic protein that activates white blood cells which effect a cell-mediated immune response, which white blood cells are sensitized to an antigenic Eimeria protein.

摘要翻译： 公开了编码抗原蛋白质的DnA序列，鉴定此类DNA序列的方法和由该DNA序列编码的抗原。 该方法的第一步是提供多个DNA序列。 然后将这些序列插入DNA表达载体中以形成重组表达载体。 将表达载体插入合适的宿主中以形成表达DNA序列的转化体。 然后将转化体与针对艾美球虫抗原的抗体接触，以鉴定含有编码艾美球虫抗原的DNA序列的转化体。 然后从鉴定为编码抗原的DNA序列产生这些抗原。 这样产生的抗原与影响细胞介导的免疫应答的白细胞接触，白血球对抗原性艾美球虫蛋白致敏，从而鉴定编码诱导细胞介导的免疫应答的抗原的DNA序列 球虫病 本发明的DNA序列包含编​​码激活影响细胞介导的免疫应答的白细胞的抗原性蛋白质的表达的克隆基因或其片段，所述白细胞对抗原性艾美球虫蛋白致敏。

公开(公告)号：US07887818B2

公开(公告)日：2011-02-15

申请号：US12070684

申请日：2008-02-20

申请人： Wenbin Tuo ,  Mark C. Jenkins ,  Yan Zhao

发明人： Wenbin Tuo ,  Mark C. Jenkins ,  Yan Zhao

IPC分类号： A61K39/012 ,  C07K14/00

CPC分类号： A61K39/002 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55561 ,  A61K2039/55566

摘要： Neospora caninum is the causal agent of bovine neosporosis which results in high levels of abortion. The protective efficacy of two Neospora antigens: Neospora cyclophilin (NcCyP) and NcSRS2 was evaluated. Mice vaccinated with recombinant (r) NcCyP, rNcSRS2, and the combination rNcCyP plus rNcSRS2, formulated with adjuvant ImmuMax-SR® and CpG were challenge-infected 3 weeks following the booster immunization and necropsied 3 weeks after the challenge infection. Mice vaccinated with rNcCyP, rNcSRS2, or the combination of rNcCyP and rNcSRS2 responded with high levels of NcCyP- or NcSRS2- specific antibodies. Mice which received vaccines formulated with either rNcCyP or the combination rNcCyP and rNcSRS2 had a higher (p

摘要翻译： 新孢子虫是牛新孢子虫病的致病因子，导致高水平的流产。 评估了两种新孢子虫抗原：新孢子虫亲环蛋白（NcCyP）和NcSRS2的保护功效。 用加强免疫3周后用重组（r）NcCyP，rNcSRS2和用佐剂ImmuMax-SR和CpG配制的rNcCyP加rNcSRS2接种的小鼠进行攻击感染，并在攻击感染后3周进行尸检。 用rNcCyP，rNcSRS2或rNcCyP和rNcSRS2的组合接种的小鼠响应高水平的NcCyP或NcSRS2特异性抗体。 使用rNcCyP或rNcCyP和rNcSRS2组合配制疫苗的小鼠与模拟或未接种疫苗的小鼠相比具有较高的（p <0.01）的保护作用。 单独使用rNcSRS2免疫的组显示略低的保护水平。 结果表明，NcCyP是一种高效的疫苗候选物，可用于保护新孢子虫感染。

公开(公告)号：US20090208519A1

公开(公告)日：2009-08-20

申请号：US12070684

申请日：2008-02-20

申请人： Wenbin Tuo ,  Mark C. Jenkins ,  Yan Zhao

发明人： Wenbin Tuo ,  Mark C. Jenkins ,  Yan Zhao

IPC分类号： A61K39/00 ,  A61P31/00

CPC分类号： A61K39/002 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55561 ,  A61K2039/55566

摘要： Neospora caninum is the causal agent of bovine neosporosis which results in high levels of abortion. The present study determined the protective efficacy of two Neospora antigens—Neospora cyclophilin (NcCyP) and NcSRS2. The ability of native NcCyP to upregulate mouse IFNγ was also confirmed in this study. Recombinant NcCyP or NcSRS2 were tested either alone or in combination and formulated with adjuvant ImmuMax-SR and CpG. Female BALB/c mice (n=15) of 10-12 weeks of age were immunized s.c. twice in a 2-week interval with vaccines containing either NcCyP alone, NcSRS2 alone, NcCyP plus NcSRS2, or non-recombinant bacterial antigen (NR) in 2 separate trials. All mice were challenge-infected 3 weeks following the booster immunization and necropsied 3 weeks after the challenge infection. Brain and serum were collected and Nc-specific DNA sequence in brain tissue and antibodies in serum were analyzed by PCR or ELISA/Western blotting. Results showed that mice vaccinated with rNcCyP, rNcSRS2, or both rNcCyP and rNcSRS2 responded with high levels of NcCyP or NcSRS2 specific antibodies. Overall, mice received vaccines formulated with either rNcCyP or rNcCyP and rNcSRS2 had a higher (p

摘要翻译： 新孢子虫是牛新孢子虫病的致病因子，导致高水平的流产。 本研究确定了两种新孢子虫抗原 - 新孢子虫亲环蛋白（NcCyP）和NcSRS2的保护功效。 在本研究中也证实了天然NcCyP上调小鼠IFNγ的能力。 单独或组合测试重组NcCyP或NcSRS2，并用佐剂ImmuMax-SR和CpG配制。 10-12周龄的雌性BALB / c小鼠（n = 15）免疫s.c. 在两个单独的试验中，在含有单独的NcCyP，单独的NcSRS2，NcCyP加NcSRS2或非重组细菌抗原（NR）的疫苗的两周间隔中两次。 所有小鼠在加强免疫后3周进行攻击感染，并在攻击感染后3周进行尸检。 收集脑和血清，通过PCR或ELISA / Western印迹分析血清中脑组织和抗体中的Nc特异性DNA序列。 结果表明，用rNcCyP，rNcSRS2或rNcCyP和rNcSRS2两者接种的小鼠均响应高水平的NcCyP或NcSRS2特异性抗体。 总体而言，小鼠接受用rNcCyP或rNcCyP配制的疫苗，当与模拟或未接种疫苗的小鼠相比时，rNcSRS2具有较高的（p <0.01）的保护。 单独使用rNcSRS2免疫的组表现出略低的保护水平，比未接种组更高（p <0.05），但与模拟接种组相比没有差异（p = 0.06）。 本研究的结果表明，NcCyP是一种非常有效的候选疫苗，可用于防止新孢子虫感染。

公开(公告)号：US06710166B1

公开(公告)日：2004-03-23

申请号：US09888501

申请日：2001-06-26

申请人： Mark C. Jenkins ,  Ron Fayer ,  James Trout

发明人： Mark C. Jenkins ,  Ron Fayer ,  James Trout

IPC分类号： A61K3800

CPC分类号： C07K14/44 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/505 ,  C07K2319/00 ,  Y02A50/488 ,  Y02A50/489

摘要： Recombinant proteins have been developed for the immunization of animals against cryptosporidiosis. The proteins are effective for the immunization of a variety of animals against Cryptosporidium parvum, particularly for the production of hyperimmune colostrum that may be used to confer passive immunity against the parasite. Isolated DNA sequences which encode these proteins have also been developed. The DNA sequences may be inserted into recombinant DNA molecules such as cloning vectors or expression vectors for the transformation of cells and the production of the proteins.

摘要翻译： 已经开发了用于免疫动物以防止隐孢子虫病的重组蛋白质。 这些蛋白质可以有效地免疫多种动物对小隐孢子虫，特别是用于生产可能被用于赋予对寄生虫的被动免疫的超免疫初乳。 已经开发了编码这些蛋白质的分离的DNA序列。 可以将DNA序列插入到重组DNA分子中，例如用于转化细胞和产生蛋白质的克隆载体或表达载体。

公开(公告)号：US06521229B1

公开(公告)日：2003-02-18

申请号：US09888655

申请日：2001-06-26

申请人： Mark C. Jenkins ,  Ronald Fayer ,  James Trout

发明人： Mark C. Jenkins ,  Ronald Fayer ,  James Trout

IPC分类号： A61K39395

CPC分类号： C07K14/44 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/505 ,  C07K2319/00 ,  Y02A50/488 ,  Y02A50/489

摘要： Recombinant proteins have been developed for the immunization of animals against cryptosporidiosis. The proteins are effective for the immunization of a variety of animals against Cryptosporidium parvum, particularly for the production of hyperimmune colostrum that may be used to confer passive immunity against the parasite. Isolated DNA sequences which encode these proteins have also been developed. The DNA sequences may be inserted into recombinant DNA molecules such as cloning vectors or expression vectors for the transformation of cells and the production of the proteins.

摘要翻译： 已经开发了用于免疫动物以防止隐孢子虫病的重组蛋白质。 这些蛋白质可以有效地免疫多种动物对小隐孢子虫，特别是用于生产可能被用于赋予对寄生虫的被动免疫的超免疫初乳。 已经开发了编码这些蛋白质的分离的DNA序列。 可以将DNA序列插入到重组DNA分子中，例如用于转化细胞和产生蛋白质的克隆载体或表达载体。

公开(公告)号：US06476192B1

公开(公告)日：2002-11-05

申请号：US08624677

申请日：1996-04-15

申请人： Nicola C. Lally ,  Mark C. Jenkins ,  Jitender P. Dubey

发明人： Nicola C. Lally ,  Mark C. Jenkins ,  Jitender P. Dubey

IPC分类号： C07K100

CPC分类号： G01N33/56905 ,  C07K14/44

摘要： Antigens for the detection of antibodies to Neospora parasites for the diagnosis of neosporosis have been identified. Recombinant antigens may be produced by expression of DNA sequences derived from Neospora caninum. Both antigens are capable of detecting antibody responses in animals experimentally inoculated with N. caninum but show no evidence of cross-reactivity with serum from animals inoculated with closely related parasites such as Toxoplasma gondii or Sarcocystis species.

摘要翻译： 已经鉴定了用于检测新孢子虫寄生虫抗体用于诊断新孢子虫病的抗原。 重组抗原可以通过表达源自新孢子虫的DNA序列产生。 两种抗原都能够检测动物实验接种卡那霉素的动物的抗体反应，但是没有证据表明与接种密切相关的寄生虫如弓形虫或蛛网膜囊虫的动物的血清交叉反应。