公开(公告)号：US5962272A

公开(公告)日：1999-10-05

申请号：US778494

申请日：1997-01-03

Applicant: Alex Chenchik ,  York Zhu ,  Luda Diatchenko ,  Paul Siebert

Inventor： Alex Chenchik ,  York Zhu ,  Luda Diatchenko ,  Paul Siebert

IPC: C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6855 ,  C12N15/1096

Abstract: The present invention pertains to methods for the synthesis and cloning of full-length cDNA, or cDNA fragments, that correspond to the complete sequence of 5'-ends of mRNA molecules. The method of the present invention comprises contacting RNA with a cDNA synthesis primer which can anneal to RNA, a suitable enzyme which possesses reverse transcriptase activity, and a template switching oligonucleotide under conditions sufficient to permit the template-dependent extension of the primer to generate an mRNA-cDNA hybrid. The template switching oligonucleotide hybridizes to the CAP site at the 5'-end of the RNA molecule and serves as a short, extended template for CAP-dependent extension of the 3'-end of the ss cDNA that is complementary to the template switching oligonucleotide. The resulting full-length ss cDNA includes the complete 5'-end of the RNA molecule as well as the sequence complementary to the template switching oligonucleotide, which can then serve as a universal priming site in subsequent amplification of the cDNA.The subject invention also pertains to the template switching oligonucleotides that can be used according to the subject method. Kits containing the template switching oligonucleotide are also included within the scope of the present invention.

Abstract translation: 本发明涉及合成和克隆与mRNA分子的5'末端的完整序列相对应的全长cDNA或cDNA片段的方法。 本发明的方法包括使RNA与可以与RNA退火的cDNA合成引物，其具有逆转录酶活性的合适的酶，以及模板切换寡核苷酸，其条件是足以允许引物的模板依赖性延伸以产生 mRNA-cDNA杂交。 模板切换寡核苷酸与RNA分子的5'末端的CAP位点杂交，作为与模板切换寡核苷酸互补的ssDNA的3'-末端的CAP依赖性延伸的短扩展模板 。 所得的全长ss cDNA包括RNA分子的完整5'-末端以及与模板切换寡核苷酸互补的序列，其随后可作为cDNA随后扩增中的通用启动位点。 本发明还涉及根据本发明方法可以使用的模板切换寡核苷酸。 含有模板切换寡核苷酸的试剂盒也包括在本发明的范围内。

公开(公告)号：US20110245208A1

公开(公告)日：2011-10-06

申请号：US13076386

申请日：2011-03-30

Applicant: Luda Diatchenko ,  William Maixner

Inventor： Luda Diatchenko ,  William Maixner

IPC: A61K31/138 ,  A61K31/485 ,  A61K31/167 ,  A61K31/135 ,  A61K31/4468 ,  A61K31/60 ,  A61K31/635 ,  A61K31/421 ,  A61K31/55 ,  A61K31/5513 ,  A61K31/57 ,  A61P29/00 ,  A61P25/06 ,  A61P1/00 ,  A61P25/00 ,  A61P19/02 ,  A61P9/10 ,  C12Q1/02

CPC classification number: A61K31/138 ,  A61K31/166 ,  A61K31/167 ,  A61K31/421 ,  A61K31/4468 ,  A61K31/485 ,  A61K31/55 ,  A61K31/5513 ,  A61K31/57 ,  A61K31/60 ,  A61K31/635 ,  A61K45/06 ,  C12Q1/6883 ,  C12Q2600/106 ,  A61K2300/00

Abstract: A method of combating a somatosensory disorder in a subject, comprising administering to the subject an effective amount of a composition comprising bupranolol and/or pharmaceutically acceptable derivative(s) thereof. Compositions useful for such administration are described, including salts, esters, solvates, etc. of tert-butyl[3-(2-chloro-5-methylphenoxy)-2-hydroxypropyl]amine, in which such salt, ester, solvate, etc. compound is in enantiomeric excess or homoenantiomeric in the R isomer thereof, or is formulated with racemic mixtures of the R and S stereoisomers of the salts, esters, solvates, etc. of tert-butyl[3-(2-chloro-5-methylphenoxy)-2-hydroxypropyl]amine. Combination therapy compositions of opioid receptor agonists and such compounds are also described. A method is disclosed of referential genotypic screening of candidate subjects in connection with therapeutic intervention using the compositions of the disclosure to combat the somatosensory disorder.

Abstract translation: 一种对抗受试者体细胞感染障碍的方法，包括向所述受试者施用有效量的包含苯丙酚和/或其药学上可接受的衍生物的组合物。 描述了可用于这种施用的组合物，包括[3-（2-氯-5-甲基苯氧基）-2-羟丙基]胺叔丁基酯的盐，酯，溶剂合物等，其中这种盐，酯，溶剂合物等 化合物在其R异构体中呈对映异构体过量或同型对映体，或者由[3-（2-氯-5-甲基 - 苯基） - 苯基） - 吡咯烷酮的盐，酯，溶剂合物等的R和S立体异构体的外消旋混合物配制， 甲基苯氧基）-2-羟丙基]胺。 还描述了阿片受体激动剂和这些化合物的组合治疗组合物。 公开了使用本公开的组合物与治疗性干预相关的候选受试者的参考基因型筛选的方法来对抗体感障碍。

公开(公告)号：US20240299448A1

公开(公告)日：2024-09-12

申请号：US18181865

申请日：2023-03-10

Applicant: Luda DIATCHENKO ,  Jeffrey MOGIL ,  Lucas Vasconcelos LIMA ,  Marc PARISIEN ,  Massimo ALLEGRI ,  Nader GHASEMLOU ,  Mohamad KARAKY

Inventor： Luda DIATCHENKO ,  Jeffrey MOGIL ,  Lucas Vasconcelos LIMA ,  Marc PARISIEN ,  Massimo ALLEGRI ,  Nader GHASEMLOU ,  Mohamad KARAKY

IPC: A61K35/15 ,  A61K31/197 ,  A61K31/485 ,  A61K31/56 ,  A61K38/18 ,  A61K38/19 ,  A61K38/20 ,  A61K38/21 ,  A61P25/04

CPC classification number: A61K35/15 ,  A61K31/197 ,  A61K31/485 ,  A61K31/56 ,  A61K38/1866 ,  A61K38/191 ,  A61K38/2006 ,  A61K38/204 ,  A61K38/2053 ,  A61K38/208 ,  A61K38/21 ,  A61P25/04

Abstract: The present invention relates to a method of preventing or treating chronic pain comprising administering to a subject, in an amount/number effective to elicit, sustain or potentiate an inflammatory process, a pro-inflammatory compound, myeloid leukocyte cells or a substance that increases the number or activity of myeloid leukocyte cells.

公开(公告)号：US08716349B2

公开(公告)日：2014-05-06

申请号：US13076386

申请日：2011-03-30

Applicant: Luda Diatchenko ,  William Maixner

Inventor： Luda Diatchenko ,  William Maixner

IPC: A61K31/135 ,  A61K31/44

CPC classification number: A61K31/138 ,  A61K31/166 ,  A61K31/167 ,  A61K31/421 ,  A61K31/4468 ,  A61K31/485 ,  A61K31/55 ,  A61K31/5513 ,  A61K31/57 ,  A61K31/60 ,  A61K31/635 ,  A61K45/06 ,  C12Q1/6883 ,  C12Q2600/106 ,  A61K2300/00

Abstract: A method of combating a somatosensory disorder in a subject, comprising administering to the subject an effective amount of a composition comprising bupranolol and/or pharmaceutically acceptable derivative(s) thereof. Compositions useful for such administration are described, including salts, esters, solvates, etc. of tert-butyl[3-(2-chloro-5-methylphenoxy)-2-hydroxypropyl]amine, in which such salt, ester, solvate, etc. compound is in enantiomeric excess or homoenantiomeric in the R isomer thereof, or is formulated with racemic mixtures of the R and S stereoisomers of the salts, esters, solvates, etc. of tert-butyl[3-(2-chloro-5-methylphenoxy)-2-hydroxypropyl]amine. Combination therapy compositions of opioid receptor agonists and such compounds are also described. A method is disclosed of referential genotypic screening of candidate subjects in connection with therapeutic intervention using the compositions of the disclosure to combat the somatosensory disorder.

公开(公告)号：US20090253585A1

公开(公告)日：2009-10-08

申请号：US12085785

申请日：2006-11-29

Applicant: Luda Diatchenko ,  William Maixner

Inventor： Luda Diatchenko ,  William Maixner

IPC: C40B30/04 ,  C40B40/06 ,  G06F19/00

CPC classification number: C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/172 ,  Y02A90/24

Abstract: Methods of predicting effective pharmacological therapies for a subject afflicted with a somatosensory disorder by determining a genotype of the subject with or without determination of psychosocial and/or neurological assessments of the subject are provided. Methods of predicting susceptibility of a subject to develop somatosensory disorders by determining a genotype of the subject with or without determination of psychosocial and/or neurological assessments of the subject are further provided.

Abstract translation: 提供了通过在有或无确定受试者的心理社会和/或神经学评估的情况下确定受试者的基因型来预测受体感障碍患者的有效药理学疗法的方法。 进一步提供了通过确定受试者的基因型来确定受试者的易感性以发展身体感觉障碍的方法，所述方法或不确定患者的心理社会和/或神经学评估。

公开(公告)号：US5962271A

公开(公告)日：1999-10-05

申请号：US582562

申请日：1996-01-03

Applicant: Alex Chenchik ,  York Zhu ,  Luda Diatchenko ,  Paul Siebert

Inventor： Alex Chenchik ,  York Zhu ,  Luda Diatchenko ,  Paul Siebert

IPC: C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6855 ,  C12N15/1096

Abstract: Described are compositions and methods which allow for the efficient addition of a defined sequence at the 3'-end of a full-length cDNA in the course of first-strand cDNA synthesis from an mRNA template. A cDNA synthesis primer that is capable of annealing to mRNA is used to prime the first strand synthesis reaction. An oligonucleotide that is linked to the 5'-end of the mRNA serves as a short, extended template such that when the reverse transcriptase enzyme reaches the 5'-end of the mRNA, the enzyme switches templates and proceeds to transcribe through the end of the linked oligonucleotide. As a result, the single-stranded cDNA product which corresponds to the full-length mRNA, will have at the 3'-end a defined sequence which is complementary to the linked oligonucleotide. A conservative element in the oligonucleotide sequence responsible for this reaction can include 3 to 5 guanylic acid residues at the 3'-end of the oligonucleotide. The subject invention provides for the increased synthesis of full-length cDNA from mRNA templates. The full-length cDNA prepared according to the present invention can then be amplified using PCR or cloned using standard procedures.

Abstract translation: 描述了允许在mRNA模板的第一链cDNA合成过程中在全长cDNA的3'-末端有效添加限定序列的组合物和方法。 使用能够对mRNA退火的cDNA合成引物用于引发第一链合成反应。 与mRNA的5'-末端连接的寡核苷酸用作短的扩展模板，使得当逆转录酶达到mRNA的5'末端时，酶切换模板并继续转录到 连接的寡核苷酸。 结果，对应于全长mRNA的单链cDNA产物将在3'末端具有与连接的寡核苷酸互补的确定的序列。 负责该反应的寡核苷酸序列中的保守元件可以在寡核苷酸的3'-末端包括3至5个鸟苷酸残基。 本发明提供了从mRNA模板增加的全长cDNA的合成。 然后可以使用PCR扩增根据本发明制备的全长cDNA或使用标准程序克隆。

公开(公告)号：US5759822A

公开(公告)日：1998-06-02

申请号：US592820

申请日：1996-01-26

Applicant: Alex Chenchik ,  Luda Diatchenko ,  Paul Siebert ,  Sergey Lukianov ,  Konstantin Lukianov ,  Nadia Gurskaya ,  Victor Tarabykin ,  Eugene Sverdlov

Inventor： Alex Chenchik ,  Luda Diatchenko ,  Paul Siebert ,  Sergey Lukianov ,  Konstantin Lukianov ,  Nadia Gurskaya ,  Victor Tarabykin ,  Eugene Sverdlov

IPC: C12N15/09 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6855 ,  C12Q1/6848

Abstract: The subject invention pertains to novel materials and methods for suppressing amplification of particular DNA fragments during polymerase chain reaction (PCR). The PCR suppression method uses novel adapters that are ligated to the end of a DNA fragment prior to PCR amplification. Upon melting and annealing, single-stranded DNA fragments having self-complementary adapters at the 5'- and 3'-ends of the strand can form suppressive "pan-like" double-stranded structures that suppress amplification of the fragments during PCR. The subject method offers improved specificity and sensitivity of PCR amplification of a target DNA and does not require target DNA sequence information. The subject invention can be adapted to a variety of highly useful PCR techniques and applications.

公开(公告)号：US5565340A

公开(公告)日：1996-10-15

申请号：US381572

申请日：1995-01-27

Applicant: Alex Chenchik ,  Luda Diatchenko ,  Paul Siebert ,  Sergey Lukianov ,  Konstantin Lukianov ,  Nadia Gurskaya ,  Victor Tarabykin ,  Eugene Sverdlov

Inventor： Alex Chenchik ,  Luda Diatchenko ,  Paul Siebert ,  Sergey Lukianov ,  Konstantin Lukianov ,  Nadia Gurskaya ,  Victor Tarabykin ,  Eugene Sverdlov

IPC: C12N15/09 ,  C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC classification number: C12Q1/6855 ,  C12Q1/6848

Abstract: The subject invention pertains to novel materials and methods for suppressing amplification of particular DNA fragments during polymerase chain reaction (PCR). The PCR suppression method uses novel adapters that are ligated to the end of a DNA fragment prior to PCR amplification. Upon melting and annealing, single-stranded DNA fragments having self-complementary adapters at the 5'- and 3'-ends of the strand can form suppressive "pan-like" double-stranded structures that suppress amplification of the fragments during PCR. The subject method offers improved specificity and sensitivity of PCR amplification of a target DNA and does not require target DNA sequence information. The subject invention can be adapted to a variety of highly useful PCR techniques and applications.

Abstract translation: 本发明涉及在聚合酶链反应（PCR）期间抑制特定DNA片段扩增的新型材料和方法。 PCR抑制方法使用在PCR扩增之前连接到DNA片段末端的新型衔接子。 在融合和退火时，在链的5'和3'端具有自互补衔接子的单链DNA片段可形成抑制PCR期间片段扩增的抑制性“泛状”双链结构。 本发明方法提供目标DNA的PCR扩增的特异性和灵敏度提高，并且不需要靶DNA序列信息。 本发明可以适用于各种非常有用的PCR技术和应用。