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    Method and computer program product for determining whether or not a test protein has thermostability
    1.
    发明授权
    Method and computer program product for determining whether or not a test protein has thermostability 失效
    用于确定测试蛋白质是否具有热稳定性的方法和计算机程序产品

    公开(公告)号：US07693664B2

    公开(公告)日：2010-04-06

    申请号：US11062833

    申请日：2005-02-23

    Applicant: Hideto Takami ,  Koki Horikoshi ,  Yoshihiro Takaki ,  Gab-Joo Chee ,  Shinro Nishi ,  Shigeru Shimamura ,  Hiroko Suzuki ,  Ikuo Uchiyama ,  Zhijun Li

    Inventor： Hideto Takami ,  Koki Horikoshi ,  Yoshihiro Takaki ,  Gab-Joo Chee ,  Shinro Nishi ,  Shigeru Shimamura ,  Hiroko Suzuki ,  Ikuo Uchiyama ,  Zhijun Li

    IPC: G01N33/48

    CPC classification number: G06F19/24

    Abstract: The invention relates to a method of judging the thermostability of a protein, comprising the steps of calculating an analytical value specific to a test protein by a principal component analysis based on the amino acid composition of the protein calculated from the data of the amino acid sequence of the protein or the nucleotide sequence of the gene and comparing the analytical value with an analytical value of a protein which is retained by a thermostable organism and corresponds to the test protein, and further relates to a program for allowing a computer to execute processing for judging the thermostability of a protein by the method, and a computer readable recording medium having recorded the program thereon.

    Abstract translation: 本发明涉及一种判断蛋白质的热稳定性的方法，包括以下步骤：基于由氨基酸序列的数据计算的蛋白质的氨基酸组成，通过主成分分析计算测试蛋白质特异性的分析值 的蛋白质或该基因的核苷酸序列，并将分析值与由热稳定性生物体保留并对应于测试蛋白质的蛋白质的分析值进行比较，并且还涉及允许计算机执行处理的程序 通过该方法判断蛋白质的热稳定性，以及在其上记录有程序的计算机可读记录介质。

    Copper tolerant yeast and pectinases produced by the yeast
    2.
    发明授权
    Copper tolerant yeast and pectinases produced by the yeast 失效
    由酵母生产的耐铜酵母和果胶酶

    公开(公告)号：US07273741B2

    公开(公告)日：2007-09-25

    申请号：US10252093

    申请日：2002-09-23

    Applicant: Fumiyoshi Abe ,  Koki Horikoshi

    Inventor： Fumiyoshi Abe ,  Koki Horikoshi

    IPC: C12N9/26 ,  C12N1/14 ,  C12N1/20 ,  C08B30/04

    CPC classification number: C12N9/2408 ,  C12P3/00 ,  C12R1/645

    Abstract: The object of the present invention is to provide a yeast which is tolerant to copper and which can incorporate copper at a high concentration, and also a method of removing or recovering copper from extracellular solution. The present invention is copper-tolerant yeast and the pectinases produced by the yeast. Particularly, the present invention is copper-tolerant yeast Cryptococcus sp. N6 strain isolated from deep-sea sediments and the pectinases produced by the yeast.

    Abstract translation: 本发明的目的是提供一种对铜具有耐受性并且可以以高浓度掺入铜的酵母，以及从细胞外溶液中除去或回收铜的方法。 本发明是耐铜酵母和由酵母生产的果胶酶。 特别地，本发明是耐铜酵母隐球菌属 从深海沉积物中分离的N6菌株和由酵母产生的果胶酶。

    Method of coring crustal core sample, and antimicrobial polymeric gel and gel material used in the method
    3.
    发明授权
    Method of coring crustal core sample, and antimicrobial polymeric gel and gel material used in the method 失效
    取芯地核心样品的方法，以及该方法中使用的抗菌聚合物凝胶和凝胶材料

    公开(公告)号：US07013993B2

    公开(公告)日：2006-03-21

    申请号：US10354789

    申请日：2003-01-30

    Applicant: Noriaki Masui ,  Shigeru Deguchi ,  Kaoru Tsujii ,  Koki Horikoshi

    Inventor： Noriaki Masui ,  Shigeru Deguchi ,  Kaoru Tsujii ,  Koki Horikoshi

    IPC: E21B49/00

    CPC classification number: E21B49/02 ,  E21B25/08 ,  G01N2001/021

    Abstract: A method of taking a crustal core sample, wherein the crustal core sample is obtained in a state coated with an antimicrobial polymeric gel formed of a polymer and an inorganic antimicrobial agent dispersed in the polymer. The inorganic antimicrobial agent is a compound containing at least one of silver, zinc or ions thereof. The inorganic antimicrobial agent is carried on a carrier material. The polymer forming the antimicrobial polymeric gel contains a hydrophilic group, and the antimicrobial polymeric gel contains the inorganic antimicrobial agent in a proportion of 0.0001 to 10.0 mass %.

    Abstract translation: 一种采取地壳芯样品的方法，其中所述地壳芯样品以涂覆有分散在聚合物中的聚合物和无机抗微生物剂形成的抗微生物聚合物凝胶的状态获得。 无机抗微生物剂是含有银，锌或其离子中的至少一种的化合物。 无机抗微生物剂载于载体材料上。 形成抗微生物聚合物凝胶的聚合物包含亲水基团，抗微生物聚合物凝胶含有0.0001至10.0质量％的无机抗微生物剂。

    Extremely halophilic methanogenic archaebacteria
    4.
    发明授权
    Extremely halophilic methanogenic archaebacteria 失效
    极度嗜盐的产甲烷古细菌

    公开(公告)号：US5350684A

    公开(公告)日：1994-09-27

    申请号：US668157

    申请日：1991-03-12

    Applicant: Naoki Nakatsugawa ,  Koki Horikoshi

    Inventor： Naoki Nakatsugawa ,  Koki Horikoshi

    IPC: C02F11/04 ,  C12N1/20 ,  C12N5/02 ,  C12P5/02 ,  C12R1/01

    CPC classification number: C12R1/01 ,  C12P5/023 ,  Y02E50/343 ,  Y10S435/822

    Abstract: Methanohalococcus alcaliphilum having an optimum growth sodium chloride concentration ranging from about 2.5 to about 3 mole are herein disclosed. The bacteria are halophilic or halophilic and alkalophilic and can be used as the microorganisms which play a central role in the methods of methane fermentation to enhance the efficiency of such a method since the use of the microorganism makes it possible to carry out the method at an alkaline pH, in the presence of salts in a high concentration and at a low temperature.

    Abstract translation: 本文公开了具有约2.5至约3摩尔的最佳生长氯化钠浓度的甲烷卤代球菌。 细菌是嗜盐的或嗜盐的和嗜碱性的，并且可以用作在甲烷发酵方法中发挥中心作用的微生物，以提高这种方法的效率，因为微生物的使用使得可以在 碱性pH，在高浓度和低温的盐存在下。

    High alkaline protease and use thereof
    8.
    发明授权
    High alkaline protease and use thereof 失效
    高碱性蛋白酶及其用途

    公开(公告)号：US08153413B2

    公开(公告)日：2012-04-10

    申请号：US11719713

    申请日：2005-11-16

    Applicant: Jie Lu ,  Zhi jun Li ,  Si Hung Vo ,  Yuji Hatada ,  Ken Takai ,  Susumu Ito ,  Koki Horikoshi

    Inventor： Jie Lu ,  Zhi jun Li ,  Si Hung Vo ,  Yuji Hatada ,  Ken Takai ,  Susumu Ito ,  Koki Horikoshi

    IPC: C12N9/00 ,  C12N9/92 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC classification number: C12N9/52

    Abstract: The invention aims to provide a novel alkaline protease having peculiar properties such as high alkali activity, resistance to surfactants and calcium-dependent thermostability and exhibiting excellent performance in highly alkaline detergents, and a gene coding for the amino acid sequence thereof. There is provided an alkaline protease with such properties that an active pH range is from 5 to 13, an optimum pH is approximately 12.6, an optimum temperature is 70° C., no activity drop by heating is observed up to 65° C. at pH 10 and the optimum temperature and the thermostability are not affected by Ca2+ ions. Specifically, there is provided, for example, an alkaline protease having an amino acid sequence constituting a mature enzyme as represented by SEQ ID NO: 3 or an amino acid sequence resulting from deletion, substitution, situs inversus arrangement, addition or insertion of a part of amino acids thereof, or derived from Alkaliphillus transvaalensis. The protease cleaves 26 peptide bonds among 29 peptide bonds of acidic insulin B-chain.

    Abstract translation: 本发明的目的在于提供一种具有高碱性，耐表面活性和钙依赖性热稳定性等特性，在高碱性洗涤剂中表现出优异性能的新型碱性蛋白酶及其氨基酸序列的编码基因。 提供了具有这样的性质的碱性蛋白酶，其活性pH范围为5至13，最佳pH为约12.6，最适温度为70℃，在65℃下不观察到加热下的活性降低 pH 10，最佳温度和热稳定性不受Ca2 +离子的影响。 具体地，例如提供了具有SEQ ID NO：3所示的构成成熟酶的氨基酸序列的氨基酸序列的碱性蛋白酶或由缺失，取代，位置反转排列，添加或插入部分 的氨基酸，或衍生自变形白菜（Alkaliphillus transvaalensis）。 蛋白酶在酸性胰岛素B链的29个肽键中切割26个肽键。

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