公开(公告)号：US20080286988A1

公开(公告)日：2008-11-20

申请号：US12022780

申请日：2008-01-30

Applicant: Koji YANAI ,  Takayuki SHIBATA ,  Shoichi ISHIKAWA

Inventor： Koji YANAI ,  Takayuki SHIBATA ,  Shoichi ISHIKAWA

IPC: H05K1/00 ,  H01R43/00

CPC classification number: H05K7/026 ,  B60T8/3675 ,  H05K1/0263 ,  H05K3/3447 ,  H05K2201/09709 ,  H05K2201/10272 ,  Y10S439/949 ,  Y10T29/49117

Abstract: An electronic control unit includes a circuit board, a case, and a group of a plurality of bus bars fixed to the case by insert molding. The bus bars each include a terminal portion having a constant-width portion, and a base portion which is wider than the constant-width portion. The base portions of the respective bus bars are arranged parallel to each other in the width direction thereof. Each bus bar is bent at a bent portion of its base portion. The terminal portion of each bus bar extends through the circuit board and is soldered to and electrically connected to a terminal connecting portion of an electrical circuit on the circuit board at a soldered portion. The bent portions of alternate bus bars are offset from the bent portions of the other bus bars in the longitudinal direction of the bus bars, whereby the soldered portions of the respective terminal portions are arranged in a staggered manner.

Abstract translation: 电子控制单元包括电路板，壳体和通过插入成型固定到壳体的多个汇流条的组。 汇流条各自包括具有恒定宽度部分的端子部分和比恒定宽度部分宽的基部部分。 各母线的基部在宽度方向上彼此平行地配置。 每个汇流条在其基部的弯曲部分处弯曲。 每个母线的端子部分延伸穿过电路板，并焊接到电连接部分的电路板上的电路的端子连接部分并与之电连接。 备用汇流条的弯曲部分在汇流条的长度方向上偏离其它汇流条的弯曲部分，由此相应端子部分的焊接部分以交错方式排列。

公开(公告)号：US20080197697A1

公开(公告)日：2008-08-21

申请号：US12071173

申请日：2008-02-15

Applicant: Takahiro Naganuma ,  Koji Yanai

Inventor： Takahiro Naganuma ,  Koji Yanai

IPC: B60T8/50 ,  B60T8/34

CPC classification number: B60T8/3675

Abstract: A hydraulic brake pressure control apparatus has a hydraulic unit having a housing, a casing fixed to the housing, and a circuit board arranged in the casing. A resilient member is disposed between the housing and the casing such that the housing and the casing are separated from each other, in order to reduce vibration transfer from the hydraulic unit to the circuit board. A part of the resilient member is also disposed between a bolt inserted into a through-hole formed in the resilient member and the casing.

Abstract translation: 液压制动压力控制装置具有液压单元，其具有壳体，壳体固定到壳体以及布置在壳体中的电路板。 弹性构件设置在壳体和壳体之间，使得壳体和壳体彼此分离，以便减少从液压单元到电路板的振动传递。 弹性构件的一部分也设置在插入形成在弹性构件的通孔中的螺栓和壳体之间。

公开(公告)号：US06337201B1

公开(公告)日：2002-01-08

申请号：US09142623

申请日：1998-09-10

Applicant: Koji Yanai ,  Akitaka Nakane ,  Hirofumi Nakamura ,  Yuko Baba ,  Akemi Watabe ,  Masao Hirayama

Inventor： Koji Yanai ,  Akitaka Nakane ,  Hirofumi Nakamura ,  Yuko Baba ,  Akemi Watabe ,  Masao Hirayama

IPC: C12N924

CPC classification number: C12Y302/01026 ,  C12N9/2431

Abstract: A novel &bgr;-fructofuranosidase gene and a &bgr;-fructofuranosidase encoded by the gene, a process for isolating a &bgr;-fructofuranosidase gene using the novel &bgr;-fructofuranosidase gene, and a novel &bgr;-fructofuranosidase obtained by this isolation process are disclosed. A novel mold fungus having no &bgr;-fructofuranosidase activity suitable for the production of &bgr;-fructofuranosidase, and a system for producing a recombinant &bgr;-fructofuranosidase using the novel mold fungus as a host is disclosed. Further, a &bgr;-fructofuranosidase variant which selectively and efficiently produces a specific fructooligosaccharide such as 1-kestose from sucrose is disclosed.

Abstract translation: 公开了一种新颖的β-呋喃果糖苷酶基因和由该基因编码的β-呋喃果糖苷酶，使用新的β-呋喃果糖苷酶基因分离β-呋喃果糖苷酶基因的方法和通过该分离方法获得的新型β-呋喃果糖苷酶。 公开了一种不具有适用于β-呋喃果糖苷酶生产的β-呋喃果糖苷酶活性的新型霉菌，以及使用该新型霉菌作为宿主生产重组β-呋喃果糖苷酶的体系。 此外，公开了一种β-蔗糖呋喃糖苷酶变体，其选择性和有效地产生特定的低聚果糖，例如来自蔗糖的1-kestose。

公开(公告)号：US08440400B2

公开(公告)日：2013-05-14

申请号：US12996108

申请日：2009-06-05

Applicant: Takeshi Murakami ,  Naomi Sumida ,  Koji Yanai

Inventor： Takeshi Murakami ,  Naomi Sumida ,  Koji Yanai

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12N15/63 ,  C12Q1/6844 ,  C12Q2545/101 ,  C12Q2531/10

Abstract: The present invention relates to a process for efficiently amplifying a giant DNA. More particularly, the present invention relates to a process for amplifying DNA in a cell, comprising amplifying the DNA as the target of amplification in the presence of DNAs selected from the following (i), (ii) and (iii): (i) DNA encoding a protein selected from the following 1), 2) and 3): 1) a protein consisting of the amino acid sequence of SEQ ID NO: 1, 2) a protein comprising an amino acid sequence which has a deletion, substitution, insertion or addition of one or more amino acids in the amino acid sequence of SEQ ID NO: 1, and 3) a protein comprising an amino acid sequence which has an identity of 90% or more to the amino acid sequence of SEQ ID NO: 1, (ii) DNA consisting of the nucleotide sequence of SEQ ID NO: 2, and (iii) DNA hybridizing to the nucleotide sequence of SEQ ID NO: 2 under stringent conditions.

Abstract translation: 本发明涉及一种有效扩增巨DNA的方法。 更具体地说，本发明涉及一种用于扩增细胞中DNA的方法，包括在选自以下（i），（ii）和（iii）的DNA存在下扩增作为扩增靶的DNA：（i） 编码选自以下1），2）和3）的蛋白质的DNA：1）由SEQ ID NO：1的氨基酸序列组成的蛋白质，2）包含氨基酸序列的蛋白质，所述氨基酸序列具有缺失，取代， 在SEQ ID NO：1的氨基酸序列中插入或添加一个或多个氨基酸，以及3）包含与SEQ ID NO：1的氨基酸序列具有90％以上相同性的氨基酸序列的蛋白质。 1，（ii）由SEQ ID NO：2的核苷酸序列组成的DNA，和（iii）在严格条件下与SEQ ID NO：2的核苷酸序列杂交的DNA。

公开(公告)号：US20110171692A1

公开(公告)日：2011-07-14

申请号：US12996108

申请日：2009-06-05

Applicant: Takeshi Murakami ,  Naomi Sumida ,  Koji Yanai

Inventor： Takeshi Murakami ,  Naomi Sumida ,  Koji Yanai

IPC: C12P19/34 ,  C12N1/21 ,  C12N1/00 ,  C07K14/36 ,  C12N15/63

CPC classification number: C12Q1/686 ,  C12N15/63 ,  C12Q1/6844 ,  C12Q2545/101 ,  C12Q2531/10

Abstract: The present invention relates to a process for efficiently amplifying a giant DNA. More particularly, the present invention relates to a process for amplifying DNA in a cell, comprising amplifying the DNA as the target of amplification in the presence of DNAs selected from the following (i), (ii) and (iii): (i) DNA encoding a protein selected from the following 1), 2) and 3): 1) a protein consisting of the amino acid sequence of SEQ ID NO: 1, 2) a protein comprising an amino acid sequence which has a deletion, substitution, insertion or addition of one or more amino acids in the amino acid sequence of SEQ ID NO: 1, and 3) a protein comprising an amino acid sequence which has an identity of 90% or more to the amino acid sequence of SEQ ID NO: 1, (ii) DNA consisting of the nucleotide sequence of SEQ ID NO: 2, and (iii) DNA hybridizing to the nucleotide sequence of SEQ ID NO: 2 under stringent conditions.

Abstract translation: 本发明涉及一种有效扩增巨DNA的方法。 更具体地说，本发明涉及一种用于扩增细胞中DNA的方法，包括在选自以下（i），（ii）和（iii）的DNA存在下扩增作为扩增靶的DNA：（i） 编码选自以下1），2）和3）的蛋白质的DNA：1）由SEQ ID NO：1的氨基酸序列组成的蛋白质，2）包含氨基酸序列的蛋白质，所述氨基酸序列具有缺失，取代， 在SEQ ID NO：1的氨基酸序列中插入或添加一个或多个氨基酸，以及3）包含与SEQ ID NO：1的氨基酸序列具有90％以上相同性的氨基酸序列的蛋白质。 1，（ii）由SEQ ID NO：2的核苷酸序列组成的DNA，和（iii）在严格条件下与SEQ ID NO：2的核苷酸序列杂交的DNA。

公开(公告)号：US20080227315A1

公开(公告)日：2008-09-18

申请号：US12076171

申请日：2008-03-14

Applicant: Shigeki Banno ,  Takahiro Naganuma ,  Koji Yanai

Inventor： Shigeki Banno ,  Takahiro Naganuma ,  Koji Yanai

IPC: H01R12/00

CPC classification number: H01R12/58 ,  H05K3/308

Abstract: A terminal adapted to be inserted into a through-hole of a board from an insertion end and electrically connected to the board, includes a body portion in a cylindrical shape, a first retaining portion outwardly extending from the body portion so as to be elastically deformable in a perpendicular direction relative to an axial direction of the body portion and contacting a first surface of the board or a boundary portion between the first surface and the through-hole, and a second retaining portion positioned at a counter-insertion end side relative to the first retaining portion, outwardly protruding from the body portion and contacting a second surface, which is an opposite surface of the first surface, or a boundary portion between the second surface and the through-hole.

Abstract translation: 适于从插入端插入板的通孔并电连接到板的端子包括圆柱形的主体部分，从主体部分向外延伸以便可弹性变形的第一保持部分 在相对于所述主体部的轴向的垂直方向上，并且接触所述基板的第一表面或所述第一表面与所述通孔之间的边界部分，以及相对于所述通孔位于相反插入端侧的第二保持部 第一保持部分，从主体部分向外突出并且接触作为第一表面的相对表面的第二表面或第二表面和通孔之间的边界部分。

公开(公告)号：US20080199938A1

公开(公告)日：2008-08-21

申请号：US12101683

申请日：2008-04-11

Applicant: Kaoru OKAKURA ,  Koji Yanai

Inventor： Kaoru OKAKURA ,  Koji Yanai

IPC: C12N9/42 ,  C07H21/04 ,  C12N15/00 ,  C12N1/00 ,  D06M16/00 ,  D21C1/00

CPC classification number: C12N9/2437 ,  C11D3/38645 ,  C12Y302/01004 ,  D21C5/005 ,  D21H17/22

Abstract: Disclosed is a novel cellulose having an amino acid sequence in which the 162nd and/or 166th amino acid residues in the amino acid sequence of cellulose NCE5 are substituted. Further, a polynucleotide encoding the novel cellulose, an expression vector containing the polynucleotide, a host cell transformed with the expression vector, and a cellulose preparation and a washing composition containing the cellulose are disclosed. The cellulose of the present invention is resistant to surfactants, and maintains a high activity even under alkaline conditions.

Abstract translation: 公开了具有氨基酸序列的新型纤维素，其中纤维素NCE5的氨基酸序列中第162位和/或第166位氨基酸残基被取代。 此外，公开了编码新型纤维素的多核苷酸，含有多核苷酸的表达载体，用表达载体转化的宿主细胞，以及含有纤维素的纤维素制剂和洗涤组合物。 本发明的纤维素耐表面活性剂，即使在碱性条件下也能保持高活性。

公开(公告)号：US20080165510A1

公开(公告)日：2008-07-10

申请号：US12003873

申请日：2008-01-03

Applicant: Kosei Nishimoto ,  Koji Yanai ,  Takahiro Naganuma

Inventor： Kosei Nishimoto ,  Koji Yanai ,  Takahiro Naganuma

IPC: H05K5/00 ,  B60T8/40

CPC classification number: H05K5/0047 ,  B60T8/3675 ,  H05K1/0263 ,  H05K3/32 ,  H05K3/3447 ,  H05K7/142 ,  H05K2201/1003 ,  H05K2201/10295 ,  H05K2201/10424 ,  H05K2201/10962

Abstract: A resin case main body includes a connector housing, a component housing, and a joint portion. A busbar includes a reinforcement plate portion which is orthogonal to a plane of a board and is parallel to an alignment direction of the connector housing, the joint portion, and the component housing. The reinforcement plate portion is formed by insert molding in the joint portion.

Abstract translation: 树脂壳体主体包括连接器壳体，部件壳体和接头部分。 母线包括加强板部分，其与板的平面正交并且平行于连接器壳体，接合部分和部件壳体的对准方向。 加强板部分通过在接头部分中嵌入成型而形成。

公开(公告)号：US07285404B1

公开(公告)日：2007-10-23

申请号：US10070387

申请日：2000-09-07

Applicant: Naoki Midoh ,  Kaoru Okakura ,  Koichi Miyamoto ,  Manabu Watanabe ,  Koji Yanai ,  Tetsuya Yasutake ,  Sato Aihara ,  Takafumi Futamura ,  Horst Kleinkauf ,  Takeshi Murakami

Inventor： Naoki Midoh ,  Kaoru Okakura ,  Koichi Miyamoto ,  Manabu Watanabe ,  Koji Yanai ,  Tetsuya Yasutake ,  Sato Aihara ,  Takafumi Futamura ,  Horst Kleinkauf ,  Takeshi Murakami

IPC: C12N9/00

CPC classification number: C12N9/00 ,  C12P21/00

Abstract: The present invention provides an enzyme that synthesizes a cyclic depsipeptide, particularly the substance 1022, and a gene thereof. A cyclic depsipeptide synthetase according to the present invention comprises (a) an amino acid sequence of SEQ ID NO: 2 or (b) a modified amino acid sequence of the amino acid sequence of SEQ ID NO: 2 that have one or more modifications selected from a substitution, a deletion, an addition and an insertion and has cyclic depsipeptide synthetase activity. A cyclic depsipeptide synthetase gene according to the present invention comprises a nucleotide sequence encoding a cyclic depsipeptide synthetase. The present invention also provides a recombinant vector and a transformant for expressing the cyclic depsipeptide synthetase, and a mass production system for the cyclic depsipeptide. The present invention further provides a method for producing the cyclic depsipeptide synthetase.

Abstract translation: 本发明提供合成环状缩肽，特别是物质1022及其基因的酶。 根据本发明的循环倍肽合成酶包含（a）SEQ ID NO：2的氨基酸序列或（b）SEQ ID NO：2的氨基酸序列的修饰的氨基酸序列，其具有一个或多个修饰选择 从取代，缺失，添加和插入，并具有循环衰老肽合成酶活性。 根据本发明的环状缩肽酶合成酶基因包含编码环状倍肽合成酶的核苷酸序列。 本发明还提供了用于表达环状缩酚酸肽合成酶的重组载体和转化体，以及用于环状缩酚酸肽的批量生产系统。 本发明还提供了一种用于制备环状缩酚酸肽合成酶的方法。

公开(公告)号：US07109018B1

公开(公告)日：2006-09-19

申请号：US10089514

申请日：2000-09-29

Applicant: Koji Yanai ,  Kaoru Okakura ,  Shohei Yasuda ,  Manabu Watanabe ,  Koichi Miyamoto ,  Naoki Midoh ,  Takeshi Murakami

Inventor： Koji Yanai ,  Kaoru Okakura ,  Shohei Yasuda ,  Manabu Watanabe ,  Koichi Miyamoto ,  Naoki Midoh ,  Takeshi Murakami

IPC: C12N1/20 ,  C12N5/10 ,  C07H21/04

CPC classification number: C12N9/0004 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P13/001 ,  C12P13/02

Abstract: An objective of the present invention is to provide a transformant altered so as to produce a secondary metabolite in which a benzene ring of the secondary product is modified at the para-position with a functional group containing a nitrogen atom. A transformant according to the present invention is a transformant of an organism producing a secondary metabolite having a benzene ring skeleton without substitution at the para-position with a functional group containing a nitrogen atom, said transformant being transformed by introducing genes involved in a biosynthetic pathway from chorismic acid to p-aminophenylpyruvic acid, including a gene encoding an amino acid sequence (SEQ ID NO: 2) having 4-amino-4-deoxychorismic acid synthase activity, a gene encoding an amino acid sequence (SEQ ID NO: 4) having 4-amino-4-deoxychorismic acid mutase activity and a gene encoding an amino acid sequence (SEQ ID NO: 6) having 4-amino-4-deoxyprephenic acid dehydrogenase activity, so as to produce a secondary metabolite having a benzene ring skeleton substituted at the para-position with a functional group containing a nitrogen atom. Another objective of the present invention is to provide a novel gene involved in the biosynthetic pathway from chorismic acid to p-aminophenylpyruvic acid. A novel gene according to the present invention comprises genes encoding the amino acid sequences of SEQ ID NOs: 2, 4 and 6 or modified sequences thereof.

Abstract translation: 本发明的目的是提供改性的转化体，以便产生次级代谢物，其中次级产物的苯环在对位上用含氮原子的官能团改性。 根据本发明的转化体是产生具有苯环骨架的次级代谢物的生物体的转化体，其在具有含氮原子的官能团的对位没有取代，所述转化体通过引入参与生物合成途径的基因而转化 包括编码氨基酸序列（SEQ ID NO：4）的氨基酸序列（SEQ ID NO：2）的编码氨基酸序列（SEQ ID NO：4）的氨基酸序列（SEQ ID NO：2）的基因的氨基苯基丙酮酸， 具有4-氨基-4-脱氧酸性变位酶活性的基因，以及编码具有4-氨基-4-脱氧蝶呤酸脱氢酶活性的氨基酸序列（SEQ ID NO：6）的基因，以产生具有苯环骨架的次级代谢物 在对位上用含有氮原子的官能团取代。 本发明的另一个目的是提供一种涉及从氯酸到对氨基苯基丙酮酸生物合成途径的新基因。 根据本发明的新基因包含编码SEQ ID NO：2,4和6的氨基酸序列的基因或其修饰序列。