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US06337201B1 &bgr;-fructofuranosidase and its gene, method of isolating &bgr;-fructofuranosidase gene, system for producing &bgr;-fructofuranosidase, and &bgr;-fructofuranosidase variant 有权
β-呋喃果糖苷酶及其基因,分离β-呋喃果糖苷酶基因的方法,β-呋喃果糖苷酶的制备和β-呋喃果糖苷酶变体

&bgr;-fructofuranosidase and its gene, method of isolating &bgr;-fructofuranosidase gene, system for producing &bgr;-fructofuranosidase, and &bgr;-fructofuranosidase variant
Abstract:
A novel &bgr;-fructofuranosidase gene and a &bgr;-fructofuranosidase encoded by the gene, a process for isolating a &bgr;-fructofuranosidase gene using the novel &bgr;-fructofuranosidase gene, and a novel &bgr;-fructofuranosidase obtained by this isolation process are disclosed. A novel mold fungus having no &bgr;-fructofuranosidase activity suitable for the production of &bgr;-fructofuranosidase, and a system for producing a recombinant &bgr;-fructofuranosidase using the novel mold fungus as a host is disclosed. Further, a &bgr;-fructofuranosidase variant which selectively and efficiently produces a specific fructooligosaccharide such as 1-kestose from sucrose is disclosed.
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