公开(公告)号：US20060163119A1

公开(公告)日：2006-07-27

申请号：US10533109

申请日：2003-10-31

Applicant: Ken Hirano ,  Yoshinobu Baba

Inventor： Ken Hirano ,  Yoshinobu Baba

IPC: B07B7/04

CPC classification number: G01N15/1456 ,  B01L3/5027 ,  B07C5/36 ,  G01N2015/149

Abstract: The present invention provides a fine-particle sorting technique that uses optical pressure (optical force) and that can be applied in place of heretofore known cell sorting techniques; and a fine-particle recovering technique therefor. The fine-particle recovering method of the present invention comprises directing a laser beam toward a flow path of fine particles in such a manner that the laser beam crosses the flow direction of the fine particles to thereby deflect the direction of movement of the fine particles to be recovered, in the direction of convergence of the laser beam. The sorting method of the present invention comprises performing flow cytometric sorting of fine particles according to the above recovering method of the present invention.

Abstract translation: 本发明提供使用光学压力（光学力）的微粒分选技术，其可以用于代替迄今为止已知的细胞分选技术; 及其微粒回收技术。 本发明的微细颗粒回收方法包括将激光束朝向细颗粒的流动路径引导，使得激光束穿过细颗粒的流动方向，从而将细颗粒的移动方向偏转到 沿着激光束的会聚方向被恢复。 本发明的分选方法包括按照本发明的上述回收方法进行细粒的流式细胞分选。

公开(公告)号：US5917282A

公开(公告)日：1999-06-29

申请号：US847261

申请日：1997-05-01

Applicant: Motohiro Suyama ,  Yoshihiko Kawai ,  Suenori Kimura ,  Ken Hirano ,  Norio Asakura ,  Tetsuya Morita

Inventor： Motohiro Suyama ,  Yoshihiko Kawai ,  Suenori Kimura ,  Ken Hirano ,  Norio Asakura ,  Tetsuya Morita

IPC: G01J1/02 ,  G01J1/42 ,  H01J40/04 ,  H01J43/04 ,  H01J43/08 ,  H01J43/28

CPC classification number: H01J40/04 ,  H01L31/1075

Abstract: This invention relates to an electron tube which stabilizes the orbits of electrons accelerated and focused by an electron lens and has a structure for effectively suppressing noise generated due to discharge. This electron tube has, at two ends of an insulating container, a cathode electrode and an anode electrode which constitute the electron lens. Particularly, in the electron tube, one end of the cathode electrode and a photocathode are supported by a conductive member arranged at one end of the insulating container, and the cathode electrode is electrically connected to the photocathode. The cathode electrode partially extends to a stem along the inner wall of the insulating container and is tapered toward the stem so that the distal end portion of the cathode electrode is separated from the inner wall of the insulating container. Therefore, the electron tube realizes, regardless of the size of the insulating container, a structure for preventing the insulating container from being charged and suppressing discharge followed by light emission between the cathode electrode and the insulating container.

Abstract translation: 本发明涉及一种电子管，其使由电子透镜加速和聚焦的电子的轨道稳定，并且具有有效抑制由放电产生的噪声的结构。 该电子管在绝缘容器的两端具有构成电子透镜的阴极电极和阳极电极。 特别地，在电子管中，阴极电极的一端和光电阴极由布置在绝缘容器的一端的导电构件支撑，并且阴极电连接到光电阴极。 阴极电极沿着绝缘容器的内壁部分地延伸到主管，并且朝向杆逐渐变细，使得阴极电极的远端部分与绝缘容器的内壁分离。 因此，不管绝缘容器的尺寸如何，电子管实现了防止绝缘容器被充电的结构，并且抑制了在阴极电极和绝缘容器之间发光的放电。

公开(公告)号：US06198221B1

公开(公告)日：2001-03-06

申请号：US09207667

申请日：1998-12-09

Applicant: Motohiro Suyama ,  Suenori Kimura ,  Norio Asakura ,  Ken Hirano ,  Yoshihiko Kawai ,  Yutaka Hasegawa ,  Tetsuya Morita

Inventor： Motohiro Suyama ,  Suenori Kimura ,  Norio Asakura ,  Ken Hirano ,  Yoshihiko Kawai ,  Yutaka Hasegawa ,  Tetsuya Morita

IPC: H01J4312

CPC classification number: H01J9/26 ,  H01J43/28 ,  H01J2231/50068

Abstract: The present invention relates to an electron tube comprising, at least, a cathode electrode, a face plate having a photocathode, and an electron entrance surface provided at a position where the electron emitted from the photocathode reaches. The object of the present invention is to provide an electron tube which can reduce its size and has a structure for improving the workability in its assembling process. In particular, the electron tube according to the present invention has a bonding ring, provided between the face plate and the cathode electrode, for bonding the face plate and the cathode electrode together. The bonding ring is made of a metal material selected from the group consisting of In, Au, Pb, alloys containing In, and alloys containing Pb.

Abstract translation: 本发明涉及一种电子管，其至少包括阴极电极，具有光电阴极的面板和设置在从光电阴极发射的电子到达的位置处的电子入射面。 本发明的目的是提供一种电子管，其可以减小其尺寸并具有用于提高其组装过程中的可加工性的结构。 特别地，根据本发明的电子管具有设置在面板和阴极之间的用于将面板和阴极接合在一起的接合环。 接合环由选自In，Au，Pb，含有In的合金和含Pb合金的金属材料制成。

公开(公告)号：US07428971B2

公开(公告)日：2008-09-30

申请号：US10533109

申请日：2003-10-31

Applicant: Ken Hirano ,  Yoshinobu Baba

Inventor： Ken Hirano ,  Yoshinobu Baba

IPC: B03B5/62

CPC classification number: G01N15/1456 ,  B01L3/5027 ,  B07C5/36 ,  G01N2015/149

Abstract: The present invention provides a fine-particle sorting technique that uses optical pressure (optical force) and that can be applied in place of heretofore known cell sorting techniques; and a fine-particle recovering technique therefor. The fine-particle recovering method of the present invention comprises directing a laser beam toward a flow path of fine particles in such a manner that the laser beam crosses the flow direction of the fine particles to thereby deflect the direction of movement of the fine particles to be recovered, in the direction of convergence of the laser beam. The sorting method of the present invention comprises performing flow cytometric sorting of fine particles according to the above recovering method of the present invention.

Abstract translation: 本发明提供使用光学压力（光学力）的微粒分选技术，其可以用于代替迄今为止已知的细胞分选技术; 及其微粒回收技术。 本发明的微细颗粒回收方法包括将激光束朝向细颗粒的流动路径引导，使得激光束穿过细颗粒的流动方向，从而将细颗粒的移动方向偏转到 沿着激光束的会聚方向被恢复。 本发明的分选方法包括按照本发明的上述回收方法进行细粒的流式细胞分选。

公开(公告)号：US20090291440A1

公开(公告)日：2009-11-26

申请号：US12301766

申请日：2006-11-16

Applicant: Ken Hirano ,  Yoshinobu Baba ,  Mitsuru Ishikawa ,  Yoshiyuki Mizushina ,  Takahiro Nishimoto

Inventor： Ken Hirano ,  Yoshinobu Baba ,  Mitsuru Ishikawa ,  Yoshiyuki Mizushina ,  Takahiro Nishimoto

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6869 ,  C12P19/34 ,  C12Q1/6874 ,  C12Q2535/101 ,  C12Q2537/149 ,  C12Q2521/543 ,  C12Q2521/101 ,  C12Q2565/518

Abstract: The present invention provides a nucleic acid synthesis method capable of continuously carrying out an extension reaction and a single molecule sequencing method capable of obtaining base information accurately at high speed. A method for synthesizing a nucleic acid, including the steps of: forming a complex of a target nucleic acid hybridized to a primer oligonucleotide and a DNA polymerase β; allowing the DNA polymerase β to incorporate a fluorescently-labeled dNTP so that the fluorescently-labeled dNTP is bound to the 3′ end of the primer oligonucleotide; and allowing the DNA polymerase β to continuously incorporate fluorescently-labeled dNTP to extend a nucleic acid complementary to the target nucleic acid from the 3′ end of the bound fluorescently-labeled dNTP. A method for sequencing a single nucleic acid molecule, including the steps of said method for synthesizing a nucleic acid, wherein fluorescence emitted from each of the fluorescently-labeled dNTP incorporated into the DNA polymerase β is sequentially detected to carry out the sequencing of the target nucleic acid.

Abstract translation: 本发明提供能够连续进行延伸反应的核酸合成方法和能够高速准确获得基础信息的单分子测序方法。 一种合成核酸的方法，包括以下步骤：形成与引物寡核苷酸杂交的靶核酸和DNA聚合酶β的复合物; 使DNA聚合酶β掺入荧光标记的dNTP，使得荧光标记的dNTP与引物寡核苷酸的3'末端结合; 并允许DNA聚合酶β连续掺入荧光标记的dNTP，以从结合的荧光标记的dNTP的3'末端延伸与靶核酸互补的核酸。 一种用于对单个核酸分子进行测序的方法，包括所述合成核酸的方法的步骤，其中顺序检测从引入到DNA聚合酶β中的每个荧光标记的dNTP发射的荧光，以进行靶的测序 核酸。

公开(公告)号：US5883466A

公开(公告)日：1999-03-16

申请号：US891840

申请日：1997-07-14

Applicant: Motohiro Suyama ,  Suenori Kimura ,  Norio Asakura ,  Ken Hirano ,  Yoshihiko Kawai ,  Yutaka Hasegawa ,  Tetsuya Morita

Inventor： Motohiro Suyama ,  Suenori Kimura ,  Norio Asakura ,  Ken Hirano ,  Yoshihiko Kawai ,  Yutaka Hasegawa ,  Tetsuya Morita

IPC: H01J43/28 ,  H01J43/12

CPC classification number: H01J43/28

Abstract: The present invention relates to an electron tube includes, at least, a cathode electrode and a face plate having a photocathode which are arranged at one end of a body, and a stem arranged at the other end of the body for defining the position of an electron entrance surface where the electron emitted from the photocathode reaches. The object of the present invention is to provide an electron tube which can reduce its size and has a structure for improving the workability in its assembling process. In particular, the electron tube in accordance with the present invention comprises a bonding ring, provided between the face plate and the cathode electrode, for bonding the face plate and the cathode electrode together. The bonding ring is made of a metal material selected from the group consisting of In, Au, Pb, alloys containing In, and alloys containing Pb.

Abstract translation: 本发明涉及一种电子管，至少包括一个阴极电极和一个具有光电阴极的面板，其布置在主体的一端，以及一个杆，设置在主体的另一端，用于限定一个 从光电阴极发射的电子到达的电子入射面。 本发明的目的是提供一种电子管，其可以减小其尺寸并具有用于提高其组装过程中的可加工性的结构。 特别地，根据本发明的电子管包括设置在面板和阴极之间的用于将面板和阴极结合在一起的接合环。 接合环由选自In，Au，Pb，含有In的合金和含Pb合金的金属材料制成。