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    Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
    2.
    发明授权
    Combinatorial DNA library for producing modified N-glycans in lower eukaryotes 有权
    用于在低等真核生物中生产修饰的N-聚糖的组合DNA文库

    公开(公告)号：US07935513B2

    公开(公告)日：2011-05-03

    申请号：US12156936

    申请日：2008-06-05

    申请人： Tillman U. Gerngross ,  Stefan Wildt ,  Byung-Kwon Choi ,  Juergen Hermann Nett ,  Piotr Bobrowicz ,  Stephen R. Hamilton ,  Robert C. Davidson

    发明人： Tillman U. Gerngross ,  Stefan Wildt ,  Byung-Kwon Choi ,  Juergen Hermann Nett ,  Piotr Bobrowicz ,  Stephen R. Hamilton ,  Robert C. Davidson

    IPC分类号： C12N1/15 ,  C12P21/00

    CPC分类号： C12P21/005 ,  C07K14/47 ,  C12N9/1051 ,  C12N15/1082 ,  C12N15/79

    摘要： The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.

    摘要翻译： 本发明涉及具有修饰的寡糖的真核宿主细胞，其可以通过异源表达一组糖基转移酶，糖转运体和甘露糖苷酶进一步修饰，以成为用于产生哺乳动物例如人治疗性糖蛋白的宿主菌株。 本发明提供核酸分子和组合文库，其可用于成功靶向和表达哺乳动物酶活性，例如参与糖基化的真核宿主细胞中的细胞内区室的那些。 该方法提供了可用于表达和靶向参与糖基化的任何所需基因的工程化宿主细胞。 建立或选择具有修饰寡糖的宿主细胞。 在工程化宿主细胞中制备的N-聚糖具有Man5GlcNAc2核心结构，然后可以通过异源表达一种或多种酶，例如糖基转移酶，糖转运蛋白和甘露糖苷酶来进一步修饰，以产生人样糖蛋白。 为了生产治疗性蛋白质，该方法可适用于工程化可能获得任何所需糖基化结构的细胞系。

    Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
    5.
    发明申请
    Combinatorial DNA library for producing modified N-glycans in lower eukaryotes 有权
    用于在低等真核生物中生产修饰的N-聚糖的组合DNA文库

    公开(公告)号：US20090209024A1

    公开(公告)日：2009-08-20

    申请号：US12156936

    申请日：2008-06-05

    申请人： Tillman U. Gerngross ,  Stefan Wildt ,  Byung-Kwon Choi ,  Juergen Hermann Nett ,  Piotr Bobrowicz ,  Stephen R. Hamilton ,  Robert C. Davidson

    发明人： Tillman U. Gerngross ,  Stefan Wildt ,  Byung-Kwon Choi ,  Juergen Hermann Nett ,  Piotr Bobrowicz ,  Stephen R. Hamilton ,  Robert C. Davidson

    IPC分类号： C12N1/19 ,  C12N1/15

    CPC分类号： C12P21/005 ,  C07K14/47 ,  C12N9/1051 ,  C12N15/1082 ,  C12N15/79

    摘要： The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.

    摘要翻译： 本发明涉及具有修饰的寡糖的真核宿主细胞，其可以通过异源表达一组糖基转移酶，糖转运体和甘露糖苷酶进一步修饰，以成为用于产生哺乳动物例如人治疗性糖蛋白的宿主菌株。 本发明提供核酸分子和组合文库，其可用于成功靶向和表达哺乳动物酶活性，例如参与糖基化的真核宿主细胞中的细胞内区室的那些。 该方法提供了可用于表达和靶向参与糖基化的任何所需基因的工程化宿主细胞。 建立或选择具有修饰寡糖的宿主细胞。 在工程化宿主细胞中制备的N-聚糖具有Man5GlcNAc2核心结构，然后可以通过异源表达一种或多种酶，例如糖基转移酶，糖转运蛋白和甘露糖苷酶来进一步修饰，以产生人样糖蛋白。 为了生产治疗性蛋白质，该方法可适用于工程化可能获得任何所需糖基化结构的细胞系。

    Combinatorial DNA Library for Producing Modified N-Glycans In Lower Eukaryotes
    6.
    发明申请
    Combinatorial DNA Library for Producing Modified N-Glycans In Lower Eukaryotes 审中-公开
    用于在低等真核生物中生产修饰的N-糖的组合DNA文库

    公开(公告)号：US20130217067A1

    公开(公告)日：2013-08-22

    申请号：US13408432

    申请日：2012-02-29

    申请人： TILLMAN U. GERNGROSS ,  Stefan Wildt ,  Byung-kwon Choi ,  Juergen Hermann Nett ,  Piotr Bobrowicz ,  Stephen R. Hamilton ,  Robert C. Davidson

    发明人： TILLMAN U. GERNGROSS ,  Stefan Wildt ,  Byung-kwon Choi ,  Juergen Hermann Nett ,  Piotr Bobrowicz ,  Stephen R. Hamilton ,  Robert C. Davidson

    IPC分类号： C07K14/47

    CPC分类号： C12P21/005 ,  C07K14/47 ,  C12N9/1051 ,  C12N15/1082 ,  C12N15/79

    摘要： The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yield human-like glycoproteins.

    摘要翻译： 本发明涉及具有修饰的寡糖的真核宿主细胞，其可以通过异源表达一组糖基转移酶，糖转运体和甘露糖苷酶进一步修饰，以成为用于产生哺乳动物例如人治疗性糖蛋白的宿主菌株。 本发明提供核酸分子和组合文库，其可用于成功靶向和表达哺乳动物酶活性，例如参与糖基化的真核宿主细胞中的细胞内区室的那些。 该方法提供了可用于表达和靶向参与糖基化的任何所需基因的工程化宿主细胞。 建立或选择具有修饰寡糖的宿主细胞。 在工程化宿主细胞中制备的N-聚糖具有Man5GlcNAc2核心结构，然后可以通过异源表达一种或多种酶，例如糖基转移酶，糖转运蛋白和甘露糖苷酶来进一步修饰，以产生人样糖蛋白。

    Combinatorial DNA Library for Producing Modified N-Glycans in Lower Eukaryotes
    7.
    发明申请
    Combinatorial DNA Library for Producing Modified N-Glycans in Lower Eukaryotes 审中-公开
    用于在低等真核生物中生产修饰的N-糖的组合DNA文库

    公开(公告)号：US20120052530A1

    公开(公告)日：2012-03-01

    申请号：US13156804

    申请日：2011-06-09

    申请人： TILLMAN U. GERNGROSS ,  Stefan Wildt ,  Byung-kwon Choi ,  Juergen Hermann Nett ,  Piotr Bobrowicz ,  Stephen R. Hamilton ,  Robert C. Davidson

    发明人： TILLMAN U. GERNGROSS ,  Stefan Wildt ,  Byung-kwon Choi ,  Juergen Hermann Nett ,  Piotr Bobrowicz ,  Stephen R. Hamilton ,  Robert C. Davidson

    IPC分类号： C12P21/00 ,  C12N1/19 ,  C40B40/08

    CPC分类号： C12P21/005 ,  C07K14/47 ,  C12N9/1051 ,  C12N15/1082 ,  C12N15/79

    摘要： The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.

    摘要翻译： 本发明涉及具有修饰的寡糖的真核宿主细胞，其可以通过异源表达一组糖基转移酶，糖转运体和甘露糖苷酶进一步修饰，以成为用于产生哺乳动物例如人治疗性糖蛋白的宿主菌株。 本发明提供核酸分子和组合文库，其可用于成功靶向和表达哺乳动物酶活性，例如参与糖基化的真核宿主细胞中的细胞内区室的那些。 该方法提供了可用于表达和靶向参与糖基化的任何所需基因的工程化宿主细胞。 建立或选择具有修饰寡糖的宿主细胞。 在工程化宿主细胞中制备的N-聚糖具有Man5GlcNAc2核心结构，然后可以通过异源表达一种或多种酶，例如糖基转移酶，糖转运蛋白和甘露糖苷酶来进一步修饰，以产生人样糖蛋白。 为了生产治疗性蛋白质，该方法可适用于工程化可能获得任何所需糖基化结构的细胞系。

    Production of modified glycoproteins having multiple antennary structures
    8.
    发明申请
    Production of modified glycoproteins having multiple antennary structures 审中-公开
    具有多个触角结构的修饰的糖蛋白的生产

    公开(公告)号：US20090226959A1

    公开(公告)日：2009-09-10

    申请号：US12313636

    申请日：2008-11-21

    申请人： Piotr Bobrowicz ,  Stephen R. Hamilton ,  Tillman U. Gerngross ,  Stefan Wildt ,  Byung-Kwon Choi ,  Juergen Hermann Nett ,  Robert C. Davidson

    发明人： Piotr Bobrowicz ,  Stephen R. Hamilton ,  Tillman U. Gerngross ,  Stefan Wildt ,  Byung-Kwon Choi ,  Juergen Hermann Nett ,  Robert C. Davidson

    IPC分类号： C12P21/00 ,  C12N1/19 ,  C12N1/15 ,  C12N15/63 ,  C07K2/00

    CPC分类号： C12P21/005 ,  C12N9/1051

    摘要： The present invention relates to eukaryotic host cells, especially lower eukaryotic host cells, having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar and sugar nucleotide transporters to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells exhibit GnTIII, GnTIV, GnTV, GnT VI or GnTIX activity, which produce bisected and/or multiantennary N-glycan structures and may be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar, sugar nucleotide transporters, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.

    摘要翻译： 本发明涉及具有修饰的寡糖的真核宿主细胞，特别是较低的真核宿主细胞，其可以通过异源表达一组糖基转移酶，糖和糖核苷酸转运蛋白进一步修饰，以成为用于产生哺乳动物的宿主菌株， 人类治疗糖蛋白。 该方法提供了可用于表达和靶向参与糖基化的任何所需基因的工程化宿主细胞。 制备或选择具有修饰的脂质连接寡糖的宿主细胞。 在工程化宿主细胞中制备的N-聚糖表现出GnTIII，GnTIV，GnTV，GnT VI或GnTIX活性，其产生二等分和/或多元N-聚糖结构，并且可以通过异源表达一种或多种酶，例如糖基转移酶 ，糖，糖核苷酸转运蛋白，以产生人样糖蛋白。 为了生产治疗性蛋白质，该方法可适用于工程化可能获得任何所需糖基化结构的细胞系。

    Device and method of designing knit products to be manufactured on a knitting machine
    9.
    发明授权
    Device and method of designing knit products to be manufactured on a knitting machine 失效
    在针织机上制作针织产品的设备和方法

    公开(公告)号：US06611730B1

    公开(公告)日：2003-08-26

    申请号：US09483953

    申请日：2000-01-14

    申请人： Thomas Stoll ,  Juergen Hermann ,  Helmut Bruendl ,  Horst Fries ,  Hermann Weiss ,  Michael Haug ,  Karsten Stello ,  Gerhard Ertl

    发明人： Thomas Stoll ,  Juergen Hermann ,  Helmut Bruendl ,  Horst Fries ,  Hermann Weiss ,  Michael Haug ,  Karsten Stello ,  Gerhard Ertl

    IPC分类号： G06F1900

    CPC分类号： D04B37/02 ,  D04B7/26 ,  D04B7/28

    摘要： A device for designing knit products to be manufactured on a knitting machine includes at least one storage device for storing data required for the production of the knit products on the knitting machine; at least one display device for displaying design images for the knit products; and at least one input device for altering the design images. Data to be exchanged between the storage, input and display devices is processed to generate at least one knit image presentation and at least one corresponding thread course presentation for display as the design images on the display device, and one of the presentations is simultaneously correspondingly altered as the other is being altered by using the input device.

    摘要翻译： 用于设计在针织机上制造针织产品的装置包括至少一个存储装置，用于存储在针织机上生产针织产品所需的数据; 至少一个用于显示针织产品的设计图像的显示装置; 以及用于改变设计图像的至少一个输入装置。 在存储，输入和显示设备之间进行交换的数据被处理以产生至少一个针织图像呈现和至少一个对应的线程课程呈现，用于在显示设备上显示为设计图像，并且一个演示文稿同时被相应地改变 因为另一个是通过使用输入设备来改变的。

    Microprocessor controlled sensor signal conditioning circuit
    10.
    发明授权
    Microprocessor controlled sensor signal conditioning circuit 失效
    微处理器控制传感器信号调理电路

    公开(公告)号：US5764541A

    公开(公告)日：1998-06-09

    申请号：US575792

    申请日：1995-12-22

    申请人： Juergen Hermann ,  Richard Bruce Kash

    发明人： Juergen Hermann ,  Richard Bruce Kash

    IPC分类号： G01L19/04 ,  G01D3/024 ,  G01D3/028 ,  G01D18/00 ,  G01G5/06 ,  G01L27/00 ,  G01C25/00

    CPC分类号： G01G5/06

    摘要： A method and apparatus for measurement signal compensation comprises providing an analog-to-digital converter that includes a dual slope integrator. A microprocessor controls the reference voltage applied to the integrator. An amplifier having a switchable gain, controls the amplification of the input signal to the integrator during the various phases of integration. An off-set compensation value is stored in a memory device for providing off-set compensation by charging a capacitor connected to the integrator. A full-scale rough adjust value is also stored on the memory device and is used as a specific reference to produce a reference voltage that is, in turn, used in the integrator during the negative slope phase. During the positive slope phase, the integration time is controlled by means of full-scale fine adjust values. The temperature dependent full-scale fine adjust values are produced from the preprogrammed values in memory by using interpolation techniques. For example, in the case of a piezo-resistive pressure sensor application, the temperature sensing is undertaken by intermittently sensing the temperature of the measuring circuitry using an on-chip temperature sensor, or the temperature at the top of the sensor bridge using the pressure sensors, or by using external temperature sensors. Pressure measurement depends on the plus and minus outputs of the sensor bridge, and measurement is performed by appropriately adjusting the integrator to provide for temperature compensation during the analog-to-digital conversion process.

    摘要翻译： 用于测量信号补偿的方法和装置包括提供包括双斜率积分器的模数转换器。 微处理器控制施加到积分器的参考电压。 具有可切换增益的放大器在集成的各个阶段期间控制对积分器的输入信号的放大。 偏移补偿值存储在存储器件中，用于通过对连接到积分器的电容器进行充电来提供偏置补偿。 全面的粗略调整值也存储在存储器件中，并且用作特定参考以产生在负斜率阶段期间在积分器中使用的参考电压。 在正斜率阶段，通过满量程微调值控制积分时间。 通过使用插值技术，通过存储器中的预编程值产生与温度相关的全刻度微调值。 例如，在压电压力传感器应用的情况下，通过使用片上温度传感器间歇地感测测量电路的温度或使用压力传感器桥顶部温度进行温度感测 传感器，或使用外部温度传感器。 压力测量取决于传感器桥的正，负输出，通过适当调整积分器来进行测量，以在模数转换过程中提供温度补偿。