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公开(公告)号：US08445581B2
公开(公告)日：2013-05-21
申请号：US12309794
申请日：2007-08-02
Applicant: Pathiraja Arachchillage Gunatillake , Roshan Tyrrel Anton Mayadunne , Raju Adhikari , Heng Chy Taing , Tam Phuong Thi Le , Jerome Anthony Werkmeister , John Alan Maurice Ramshaw , Jacinta Frances White , Tracy Ann Tebb
Inventor： Pathiraja Arachchillage Gunatillake , Roshan Tyrrel Anton Mayadunne , Raju Adhikari , Heng Chy Taing , Tam Phuong Thi Le , Jerome Anthony Werkmeister , John Alan Maurice Ramshaw , Jacinta Frances White , Tracy Ann Tebb
IPC: C08F290/02 , C08F299/00 , C08G63/08 , C08L33/04
CPC classification number: C08G18/4277 , A61L27/16 , C08F290/00 , C08F290/02 , C08F299/00 , C08G18/10 , C08G18/4833 , C08G18/771 , C08G18/8116 , C08G63/08 , C08G63/664 , C08G2230/00 , C08L53/00 , C08L53/005 , C08L53/02 , C08L2666/02
Abstract: The present invention provides a biocompatible prepolymer comprising hydrophilic and hydrophobic segments, wherein the hydrophobic segments have at least one ethylenically unsaturated functional group and at least 5% of the segments have two or more ethylenically unsaturated functional groups and water. The invention further provides a biocompatible prepolymer composition comprising hydrophilic and hydrophobic prepolymers, wherein at least one of the hydrophobic prepolymers has at least one ethylenically unsaturated functional group and at least 5% of the prepolymers have two or more ethylenically unsaturated functional groups and water. The invention further provides use of the prepolymer or prepolymer compositions of the invention in biomedical applications such as tissue engineering, as bone substitutes or scaffolds, and in wound treatment.
Abstract translation: 本发明提供了包含亲水和疏水链段的生物相容性预聚物，其中疏水链段具有至少一个烯键式不饱和官能团，并且至少5％的链段具有两个或多个烯键式不饱和官能团和水。 本发明还提供了包含亲水和疏水预聚物的生物相容性预聚物组合物，其中至少一种疏水性预聚物具有至少一个烯键式不饱和官能团，并且至少5％的预聚物具有两个或更多个烯属不饱和官能团和水。 本发明进一步提供本发明的预聚物或预聚物组合物在生物医学应用如组织工程，骨替代物或支架中的用途，以及在伤口治疗中的用途。
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公开(公告)号：US20090324675A1
公开(公告)日：2009-12-31
申请号：US12309794
申请日：2007-08-02
Applicant: Pathiraja Arachchillage Gunatillake , Roshan Tyrrel Anton Mayadunne , Raju Adhikari , Heng Chy Taing , Tam Phuong Thi Le , Jerome Anthony Werkmeister , John Alan Maurice Ramshaw , Jacinta Frances White , Tracy Ann Tebb
Inventor： Pathiraja Arachchillage Gunatillake , Roshan Tyrrel Anton Mayadunne , Raju Adhikari , Heng Chy Taing , Tam Phuong Thi Le , Jerome Anthony Werkmeister , John Alan Maurice Ramshaw , Jacinta Frances White , Tracy Ann Tebb
CPC classification number: C08G18/4277 , A61L27/16 , C08F290/00 , C08F290/02 , C08F299/00 , C08G18/10 , C08G18/4833 , C08G18/771 , C08G18/8116 , C08G63/08 , C08G63/664 , C08G2230/00 , C08L53/00 , C08L53/005 , C08L53/02 , C08L2666/02
Abstract: The present invention provides a biocompatible prepolymer comprising hydrophilic and hydrophobic segments, wherein the hydrophobic segments have at least one ethylenically unsaturated functional group and at least 5% of the segments have two or more ethylenically unsaturated functional groups and water. The invention further provides a biocompatible prepolymer composition comprising hydrophilic and hydrophobic prepolymers, wherein at least one of the hydrophobic prepolymers has at least one ethylenically unsaturated functional group and at least 5% of the prepolymers have two or more ethylenically unsaturated functional groups and water. The invention further provides use of the prepolymer or prepolymer compositions of the invention in biomedical applications such as tissue engineering, as bone substitutes or scaffolds, and in wound treatment.
Abstract translation: 本发明提供了包含亲水和疏水链段的生物相容性预聚物，其中疏水链段具有至少一个烯键式不饱和官能团，并且至少5％的链段具有两个或多个烯键式不饱和官能团和水。 本发明还提供了包含亲水和疏水预聚物的生物相容性预聚物组合物，其中至少一种疏水性预聚物具有至少一个烯键式不饱和官能团，并且至少5％的预聚物具有两个或更多个烯属不饱和官能团和水。 本发明进一步提供本发明的预聚物或预聚物组合物在生物医学应用如组织工程，骨替代物或支架中的用途，以及在伤口治疗中的用途。
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Patent Agency Ranking
公开(公告)号：US09216235B2
公开(公告)日：2015-12-22
申请号：US12673400
申请日：2008-08-14
Applicant: Alan George Brownlee , Christopher Malcolm Elvin , Jerome Anthony Werkmeister , John Alan Maurice Ramshaw , Charles Mark Lindall
Inventor： Alan George Brownlee , Christopher Malcolm Elvin , Jerome Anthony Werkmeister , John Alan Maurice Ramshaw , Charles Mark Lindall
CPC classification number: A61L24/10 , A61L24/001 , A61L24/0026 , A61L24/102 , A61L24/106 , A61L24/108 , A61L26/0028 , A61L26/0061 , A61L26/0076 , A61L27/22 , A61L27/50 , C08H1/00 , C09J189/00
Abstract: A method of crosslinking a protein or peptide for use as a biomaterial, the method comprising the step of irradiating a photoactivatable metal-ligand complex and an electron acceptor in the presence of the protein or peptide, thereby initiating a crosslinking reaction to form a 3-dimensional matrix of the biomaterial.
Abstract translation: 交联用作生物材料的蛋白质或肽的方法，所述方法包括在蛋白质或肽存在下照射可光活化的金属 - 配体复合物和电子受体的步骤，由此引发交联反应以形成3- 生物材料的三维矩阵。
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4.发明授权Method for producing, in yeast, a hydroxylated triple helical protein, and yeast host cells useful in said method 失效在酵母中生产羟基化三螺旋蛋白的方法和用于所述方法的酵母宿主细胞公开(公告)号：US06451557B1
公开(公告)日：2002-09-17
申请号：US09297269
申请日：1999-04-28
Applicant: Paul Richard Vaughan , Maria Galanis , John Alan Maurice Ramshaw , Jerome Anthony Werkmeister
Inventor： Paul Richard Vaughan , Maria Galanis , John Alan Maurice Ramshaw , Jerome Anthony Werkmeister
IPC: C12P2102
CPC classification number: C12N9/0071 , A61K38/00 , C07K14/78 , C12P21/02
Abstract: A method for producing a hydroxylated triple helical protein is disclosed, as well as a yeast host cell used in the method. The method comprises culturing a yeast host cell comprising: (1) a first DNA molecule comprising a DNA sequence encoding P4H&agr; subunit operably linked to a promoter functional in the yeast host cell, (2) a second DNA molecule comprising a DNA sequence encoding P4H&agr; subunit operably linked to a promoter functional in the yeast host cell, and (3) a third DNA molecule comprising a DNA sequence encoding a polypeptide or peptide operably linked to a promoter functional in the yeast host cell, wherein the polypeptide or peptide is one which, when hydroxylated, forms the hydroxylated triple helical protein; and wherein during culturing, each of the first DNA molecule, the second DNA molecule and the third DNA molecule are replicated, stably retained and segregated.
Abstract translation: 公开了一种生产羟基化三螺旋蛋白的方法，以及用于该方法的酵母宿主细胞。 该方法包括培养酵母宿主细胞，其包括：（1）第一DNA分子，其包含编码与在酵母宿主细胞中有功能的启动子可操作地连接的P4Halpha亚基的DNA序列，（2）第二DNA分子，其包含编码P4Halpha亚单位的DNA序列 连接到在酵母宿主细胞中起促进作用的启动子，和（3）第三DNA分子，其包含可操作地连接到在酵母宿主细胞中有功能的启动子的多肽或肽的DNA序列，其中多肽或肽是， 当羟基化时，形成羟基化三螺旋蛋白; 并且其中在培养过程中，复制，稳定地保留和分离第一DNA分子，第二DNA分子和第三DNA分子。
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公开(公告)号：US20110190813A1
公开(公告)日：2011-08-04
申请号：US12673400
申请日：2008-08-14
Applicant: Alan George Brownlee , Christopher Malcolm Elvin , Jerome Anthony Werkmeister , John Alan Maurice Ramshaw , Charles Mark Lindall
Inventor： Alan George Brownlee , Christopher Malcolm Elvin , Jerome Anthony Werkmeister , John Alan Maurice Ramshaw , Charles Mark Lindall
IPC: A61B17/08 , C07K14/00 , C07K14/75 , C07K14/78 , C07K14/435 , C07K1/00 , C07K14/765
CPC classification number: A61L24/10 , A61L24/001 , A61L24/0026 , A61L24/102 , A61L24/106 , A61L24/108 , A61L26/0028 , A61L26/0061 , A61L26/0076 , A61L27/22 , A61L27/50 , C08H1/00 , C09J189/00
Abstract: A method of crosslinking a protein or peptide for use as a biomaterial, the method comprising the step of irradiating a photoactivatable metal-ligand complex and an electron acceptor in the presence of the protein or peptide, thereby initiating a crosslinking reaction to form a 3-dimensional matrix of the biomaterial.
Abstract translation: 交联用作生物材料的蛋白质或肽的方法，所述方法包括在蛋白质或肽存在下照射可光活化的金属 - 配体复合物和电子受体的步骤，由此引发交联反应以形成3- 生物材料的三维矩阵。
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公开(公告)号：US20090098177A1
公开(公告)日：2009-04-16
申请号：US12292169
申请日：2008-11-13
Applicant: Jerome Anthony Werkmeister , Wei-Bor Tsai , John Alan Maurice Ramshaw , Helmut Werner Thissen , Ken-Yuan Chang
Inventor： Jerome Anthony Werkmeister , Wei-Bor Tsai , John Alan Maurice Ramshaw , Helmut Werner Thissen , Ken-Yuan Chang
CPC classification number: A61L27/3886 , A61K35/12 , A61L27/3608 , A61L27/3804 , A61L27/3817 , A61L27/3852 , A61L27/3895 , A61L27/52 , A61L2400/06 , A61L2430/06 , C12N5/0075 , C12N5/0653 , C12N5/0654 , C12N5/0655 , C12N5/0656 , C12N2533/40 , C12N2533/54
Abstract: Methods for treating diseased or damaged tissue in a subject are disclosed, involving administering to said subject at a site wherein diseased or damaged tissue occurs, cells of a type(s) normally found in healthy tissue corresponding to the diseased or damaged tissue, and/or suitable progenitor cells thereof, in association with bioresorbable beads or particles and optionally a gel and/or gel-forming substance. Where the cells an/or suitable progenitor cells thereof are chondrocytes, embryonic stem cells and/or bone marrow stromal cells, the methods of the invention are suitable for treating, for example, articular cartilage degeneration associated with primary osteoarthritis. Also disclosed is a device having tissue-like characteristics for treating diseased or damaged tissue in a subject, wherein the device comprises cells of a type(s) normally found in healthy tissue corresponding to the diseased or damaged tissue, and/or suitable progenitor cells thereof, in association with bioresorbable beads or particles and optionally a gel and/or gel-forming substance.
Abstract translation: 公开了用于治疗受试者中的患病或受损组织的方法，包括在发生病变或损伤的组织的部位施用所述受试者，通常在对应于患病或损伤的组织的健康组织中发现的细胞和/ 或其合适的祖细胞，其与生物可再吸收的珠粒或颗粒以及任选的凝胶和/或凝胶形成物质结合。 在细胞/或其合适的祖细胞是软骨细胞，胚胎干细胞和/或骨髓基质细胞的情况下，本发明的方法适用于治疗例如与原发性骨关节炎相关的关节软骨变性。 还公开了一种具有用于治疗受试者中的患病或受损组织的组织样特征的装置，其中该装置包括通常存在于对应于患病或受损组织的健康组织中的类型的细胞和/或合适的祖细胞 与生物可再吸收的珠粒或颗粒以及任选的凝胶和/或凝胶形成物质结合。
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7.发明申请Method for producing, in yeast, a hydroxylated triple helical protein, and yeast host cells useful in said method 审中-公开在酵母中生产羟基化三螺旋蛋白的方法和用于所述方法的酵母宿主细胞公开(公告)号：US20080139477A1
公开(公告)日：2008-06-12
申请号：US11979178
申请日：2007-10-31
Applicant: Paul Richard Vaughan , Maria Galanis , John Alan Maurice Ramshaw , Jerome Anthony Werkmeister
Inventor： Paul Richard Vaughan , Maria Galanis , John Alan Maurice Ramshaw , Jerome Anthony Werkmeister
CPC classification number: C12N9/0071 , A61K38/00 , C07K14/78 , C12P21/02
Abstract: The invention relates to a method for producing hydroxylated triple helical proteins in yeast host cells by introducing to a suitable yeast host cell, DNA sequences encoding the triple helical protein as well as prolyl 4-hydroxylase (PH4), in a manner wherein the introduced DNA sequences are replicated, stably retained and segregated by the yeast host cells.
Abstract translation: 本发明涉及通过将合适的酵母宿主细胞，编码三螺旋蛋白的DNA序列以及脯氨酰4-羟化酶（PH4）引入酵母宿主细胞中来生产羟基化三螺旋蛋白的方法，其中引入的DNA 序列被酵母宿主细胞复制，稳定保留和分离。
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