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1.发明授权公开(公告)号：US09279112B2
公开(公告)日：2016-03-08
申请号：US12713516
申请日：2010-02-26
申请人： Brian R. Scott , Patrick St-Pierre , James Lavigne , Nabil Masri , Theresa C. White , John J. Tomashek
发明人： Brian R. Scott , Patrick St-Pierre , James Lavigne , Nabil Masri , Theresa C. White , John J. Tomashek
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14
摘要： Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acid sequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolyzing cellulose with the modified cellulases in the presence of lignin.
摘要翻译： 提供了在木质素存在下表现出增加的纤维素水解活性的修饰的纤维素酶和/或包含修饰的连接肽的修饰的连接肽，其包含一个或多个氨基酸取代，插入或缺失，导致（a）计算的 接头肽的等电点和/或（b）相对于衍生了所述修饰的接头肽的亲本接头肽，接头肽中苏氨酸：丝氨酸的比例增加。 还提供了包含编码修饰的纤维素酶的核酸序列的遗传构建体，从宿主菌株制备修饰的纤维素酶的方法和在木质素存在下用修饰的纤维素酶水解纤维素的方法。
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公开(公告)号：US08263379B2
公开(公告)日：2012-09-11
申请号：US12504070
申请日：2009-07-16
IPC分类号： C12N9/24 , C12N1/00 , C12P21/06 , C12P7/06 , C07H21/04 , C07H1/00 , C07K1/00 , C12C11/00 , A21D2/00 , A23B7/00
CPC分类号： C12N9/2437 , C12N1/22 , C12N9/244 , C12Y302/01006 , C12Y302/01091 , Y02E50/17
摘要： A modified Family 6 glycosidase enzyme comprising amino acid substitutions at one or more positions selected from the group 182, 367, 399, 400 and 427 is provided (the position determined form alignment of a parental Family 6 glycosidase with SEQ ID NO: 1). Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified Family 6 glycosidase are also provided. Family 6 glycosidase of the invention display decreased hydrolysis activity of beta 1-4 linked polysaccharides and increased hydrolysis activity of beta 1-3, 1-4 linked polysaccharides compared with a parental Family 6 glycosidase. Such glycosidases find use in a variety of applications in industry, e.g., in hydrolysis of beta 1-3, 1-4 linked polysaccharides during the processing of cereal grains or the production of alcohol, animal feed or food products.
摘要翻译： 提供了修饰的家族6糖苷酶，其包含在选自182,367,399,440和427中的一个或多个位置上的氨基酸取代（通过亲本6族糖苷酶与SEQ ID NO：1的比对确定的位置）。 还提供了包含编码修饰的家族6糖苷酶的核酸序列的遗传构建体和遗传修饰微生物。 本发明的家族6糖苷酶与亲本6族糖苷酶相比，显示β1-4连接多糖的水解活性降低，β1-3,1-4连接多糖的水解活性增加。 这样的糖苷酶可用于工业中的各种应用，例如在谷物加工过程中水解β1-3,1-连接的多糖或生产醇，动物饲料或食品。
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公开(公告)号：US08486683B2
公开(公告)日：2013-07-16
申请号：US12790079
申请日：2010-05-28
申请人： Brian R. Scott , Chengsong Liu , James Lavigne , John J. Tomashek
发明人： Brian R. Scott , Chengsong Liu , James Lavigne , John J. Tomashek
CPC分类号： C12N1/22 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , Y02E50/16
摘要： Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improvements in one or more kinetic parameters (KG, KG2, kcat) comprising amino acid substitutions at one or more of positions 43, 101, 260 and 543. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.
摘要翻译： 提供衍生自里氏木霉Cel3Aβ-葡糖苷酶的修饰的β-葡糖苷酶，其在一个或多个位置43,101,260和/或其中包含氨基酸取代的一个或多个动力学参数（KG，KG2，kcat）表现出改善， 还提供了包含编码修饰的β-葡糖苷酶的核苷酸序列的遗传构建体，用于从宿主菌株产生修饰的β-葡糖苷酶的方法，以及在纤维素水解中使用经修饰的β-葡糖苷酶。
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公开(公告)号：US20100093040A1
公开(公告)日：2010-04-15
申请号：US12550615
申请日：2009-08-31
CPC分类号： C12P19/14 , C12N9/2445 , C12Y302/01021
摘要： Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improved stability at low pH, low pH and high aeration, low pH and high agitation, or low pH and elevated temperature. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.
摘要翻译： 提供了源自里氏木霉Cel3Aβ-葡糖苷酶的修饰的β-葡糖苷酶，其在低pH，低pH和高通气，低pH和高搅拌，或低pH和升高的温度下表现出改善的稳定性。 还提供了包含编码修饰的β-葡糖苷酶的核苷酸序列的遗传构建体，用于从宿主菌株产生修饰的β-葡糖苷酶的方法和在纤维素水解中使用经修饰的β-葡糖苷酶。
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公开(公告)号：US20100016570A1
公开(公告)日：2010-01-21
申请号：US12504070
申请日：2009-07-16
CPC分类号： C12N9/2437 , C12N1/22 , C12N9/244 , C12Y302/01006 , C12Y302/01091 , Y02E50/17
摘要： A modified Family 6 glycosidase enzyme comprising amino acid substitutions at one or more positions selected from the group 182, 367, 399, 400 and 427 is provided (the position determined form alignment of a parental Family 6 glycosidase with SEQ ID NO: 1). Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified Family 6 glycosidase are also provided. Family 6 glycosidase of the invention display decreased hydrolysis activity of beta 1-4 linked polysaccharides and increased hydrolysis activity of beta 1-3, 1-4 linked polysaccharides compared with a parental Family 6 glycosidase. Such glycosidases find use in a variety of applications in industry, e.g., in hydrolysis of beta 1-3, 1-4 linked polysaccharides during the processing of cereal grains or the production of alcohol, animal feed or food products.
摘要翻译： 提供了修饰的家族6糖苷酶，其包含在选自182,367,399,440和427中的一个或多个位置上的氨基酸取代（通过亲本6族糖苷酶与SEQ ID NO：1的比对确定的位置）。 还提供了包含编码修饰的家族6糖苷酶的核酸序列的遗传构建体和遗传修饰微生物。 本发明的家族6糖苷酶与亲本6族糖苷酶相比，显示β1-4连接多糖的水解活性降低，β1-3,1-4连接多糖的水解活性增加。 这样的糖苷酶可用于工业中的各种应用，例如在谷物加工过程中水解β1-3,1-连接的多糖或生产醇，动物饲料或食品。
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公开(公告)号：US20100221778A1
公开(公告)日：2010-09-02
申请号：US12713516
申请日：2010-02-26
申请人： Brian R. Scott , Patrick St-Pierre , James Lavigne , Nabil Masri , Theresa C. White , John J. Tomashek
发明人： Brian R. Scott , Patrick St-Pierre , James Lavigne , Nabil Masri , Theresa C. White , John J. Tomashek
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14
摘要： Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acidsequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolysing cellulose with the modified cellulases in the presence of lignin.
摘要翻译： 提供了在木质素存在下表现出增加的纤维素水解活性的修饰的纤维素酶和/或包含修饰的连接肽的修饰的连接肽，其包含一个或多个氨基酸取代，插入或缺失，导致（a）计算的 接头肽的等电点和/或（b）相对于衍生了所述修饰的接头肽的亲本接头肽，接头肽中苏氨酸：丝氨酸的比例增加。 还提供了包含编码修饰的纤维素酶的核酸序列的遗传构建体，从宿主菌株生产修饰的纤维素酶的方法和在木质素存在下用修饰的纤维素酶水解纤维素的方法。
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公开(公告)号：US08143049B2
公开(公告)日：2012-03-27
申请号：US12550615
申请日：2009-08-31
CPC分类号： C12P19/14 , C12N9/2445 , C12Y302/01021
摘要： Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improved stability at low pH, low pH and high aeration, low pH and high agitation, or low pH and elevated temperature. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.
摘要翻译： 提供了源自里氏木霉Cel3Aβ-葡糖苷酶的修饰的β-葡糖苷酶，其在低pH，低pH和高通气，低pH和高搅拌，或低pH和升高的温度下表现出改善的稳定性。 还提供了包含编码修饰的β-葡糖苷酶的核苷酸序列的遗传构建体，用于从宿主菌株产生修饰的β-葡糖苷酶的方法和在纤维素水解中使用经修饰的β-葡糖苷酶。
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公开(公告)号：US20100304438A1
公开(公告)日：2010-12-02
申请号：US12790079
申请日：2010-05-28
申请人： Brian R. Scott , Chengsong Liu , James Lavigne , John J. Tomashek
发明人： Brian R. Scott , Chengsong Liu , James Lavigne , John J. Tomashek
CPC分类号： C12N1/22 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , Y02E50/16
摘要： Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improvements in one or more kinetic parameters (KG, KG2, kcat) comprising amino acid substitutions at one or more of positions 43, 101, 260 and 543. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.
摘要翻译： 提供衍生自里氏木霉Cel3Aβ-葡糖苷酶的修饰的β-葡糖苷酶，其在一个或多个位置43,101,260和/或其中包含氨基酸取代的一个或多个动力学参数（KG，KG2，kcat）表现出改善， 还提供了包含编码修饰的β-葡糖苷酶的核苷酸序列的遗传构建体，用于从宿主菌株产生修饰的β-葡糖苷酶的方法，以及在纤维素水解中使用经修饰的β-葡糖苷酶。
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公开(公告)号：US20240172844A1
公开(公告)日：2024-05-30
申请号：US18071245
申请日：2022-11-29
申请人： Landon James Lavigne
发明人： Landon James Lavigne
IPC分类号： A44B11/25
CPC分类号： A44B11/25 , A44D2203/00
摘要： The present invention provides among other things a fastening apparatus for interconnecting a strap. The fastening apparatus is comprised of two interlocking members. Each interlocking member includes a first elongated portion, a second elongated portion, and bridge portion disposed between the first elongated portion and second elongated portion. One elongated portion may be longer than the other elongated portion. A ridge may be disposed along one of the elongated portions and a groove may be disposed along the other elongated portion. One or more magnets may be embedded in any of the two elongated portions or bridge portion. The fastening apparatus may be joined together by rotating one interlocking member by 180 degrees relative to the other interlocking member and sliding the two members together. The ridge and groove of one interlocking member slidably engages with the corresponding groove and corresponding ridge of the other interlocking member, respectively.
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