摘要:
A smart pet collar apparatus for pet training and safety includes a collar having a plurality of adjustment apertures extending from the collar. A buckle frame has a buckle cavity, a first buckle bar, and a second buckle bar. A prong is coupled to the second buckle bar and is selectively engageable with the plurality of adjustment apertures. A battery is coupled within the buckle cavity. A charge port, a microprocessor, a GPS tracker, a transceiver, and a plurality of control buttons are coupled to the buckle frame. The charge port is in operational communication with the battery. The GPS chip and the transceiver are in operational communication with the battery and the microprocessor. The transceiver is configured to interact with a smartphone.
摘要:
Provided are isolated cellobiohydrolases comprising a modified Glycoside Hydrolase (GH) Family 7 catalytic domain, a GH Family 7 catalytic domain and a modified Family 1 carbohydrate binding module (CBM), or both a modified Family 7 catalytic domain and a modified Family 1 CBM. Such isolated cellobiohydrolases exhibit from 45% to about 99.9% amino acid sequence identity to amino acids 1-436 of SEQ ID NO: 1 or to amino acids 1-438 of SEQ ID NO: 2 and improved activity on process substrates. Also provided are genetic constructs and genetically modified microbes for expressing the isolated cellobiohydrolases, a process for producing the isolated cellobiohydrolases, cellulase enzyme mixtures comprising the isolated cellobiohydrolase and a process for hydrolyzing a cellulosic substrate with such cellulase enzyme mixtures.
摘要翻译:提供的是分离的纤维二糖水解酶,其包含修饰的糖苷水解酶(GH)家族7催化结构域,GH家族7催化结构域和修饰的家族1碳水化合物结合模块(CBM),或修饰的家族7催化结构域和修饰的家族1 CBM两者。 这种分离的纤维二糖水解酶显示与SEQ ID NO:1的氨基酸1-436或SEQ ID NO:2的氨基酸1-438具有45%至约99.9%的氨基酸序列同一性,并且改善了对工艺底物的活性。 还提供了用于表达分离的纤维二糖水解酶的遗传构建体和遗传修饰的微生物,用于产生分离的纤维二糖水解酶的方法,包含分离的纤维二糖水解酶的纤维素酶混合物和用这种纤维素酶混合物水解纤维素底物的方法。
摘要:
The present invention provides a pin and tumbler cylinder lock and a retrofitted cylinder lock which minimizes unauthorized openings of the lock by bumping. The cylinder lock utilizes specially designed driver pins which are correlated in size to the largest possible stack height of the tumbler pins with a bump key inserted in the plug portion of the lock and the shear line height of the lock. All the driver pins are about the same height and preferably symmetrical with a lip on each end. The invention also provides a method for retrofitting existing locks by removing at least one of the driver pins and replacing the removed driver pin or pins with the driver pins of the invention.
摘要:
Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improved stability at low pH, low pH and high aeration, low pH and high agitation, or low pH and elevated temperature. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.
摘要:
An enzyme mixture for the enzymatic hydrolysis of a pretreated lignocellulosic feedstock to soluble sugars is provided. Also provided is a process for hydrolyzing a pretreated lignocellulosic feedstock with an enzyme mixture. The enzymatic hydrolysis comprises adding a cellulase enzyme mixture to a pretreated lignocellulosic feedstock. The cellulase enzyme mixture comprises a primary cellulase mixture of CBH1 and CBH2, and EG1 and EG2. The CBH1 and CBH2 being present at greater than or equal to 55% and less than 85% of the primary cellulase mixture. Furthermore, CBH2 is present at a fraction relative to CBH1 and CBH2, and EG2 is present at a fraction relative to the EG1 and EG2.
摘要:
Provided is an enzyme mixture for hydrolyzing a pretreated lignocellulosic feedstock to soluble sugars. The enzyme mixture comprises EG4 at a fractional concentration (fEG4) of about 0.25 to about 0.83 (w/w), Swollenin at a fractional concentration (fSwo1) of about 0 to about 0.66 (w/w), and Cip1 at a fractional concentration (fCip1) of 0 to about 0.33 measured relative to all accessory enzymes present in the enzyme mixture. Also provided are processes for converting a pretreated lignocellulosic feedstock to soluble sugars using the enzyme mixtures, and methods of using and producing such enzyme mixtures.
摘要:
A remotely actuated manhole system is disclosed having a cover that is pivotable relative to a frame secured with a collar on a bulk carrier, the cover being pivotable around a substantially vertical axis between and to open and closed positions. In the closed position covering an opening to a compartment in the bulk carrier, the cover is shiftable vertically between and to locked and unlocked positions by an inflatable seal member below the cover. The manhole system includes catches that prevent over-rotation of the cover from the open position to the closed position, and the catches engage in the locked position by the vertical upward movement of the cover. The inflation of the seal member and the pivoting of the cover are remotely actuated.
摘要:
The present invention provides a pin and tumbler cylinder lock and a retrofitted cylinder lock which minimizes unauthorized openings of the lock by bumping. The cylinder lock utilizes specially designed driver pins which are correlated in size to the largest possible stack height of the tumbler pins with a bump key inserted in the plug portion of the lock and the shear line height of the lock. All the driver pins are about the same height and preferably symmetrical with a lip on each end. The invention also provides a method for retrofitting existing locks by removing at least one of the driver pins and replacing the removed driver pin or pins with the driver pins of the invention.
摘要:
An improved system and technique for processing a communications session in a data communications system suitably includes the steps of receiving the communications session at a line interface card, embedding the communications session into an asynchronous transfer mode (ATM) virtual circuit at the line interface card, transporting the virtual circuit from the line interface card to a processor card via a backplane. The processor card suitably extracts the communications session from the virtual circuit, and processes the communications session as appropriate.
摘要:
A method for forming a semiconductor device comprises forming a dielectric layer over a semiconductor wafer substrate assembly having closely spaced regions, such as a memory transistor array, and widely spaced regions, such as a periphery. Under conditions specified, the dielectric layer forms to have a first thickness over the closely spaced regions and a second thickness over the widely spaced regions. The second thickness is much thinner than the first thickness and dielectric over the widely spaced regions may be etched away with a blanket etch which leaves the majority of the dielectric layer over the closely spaced regions.