公开(公告)号：US09683227B2

公开(公告)日：2017-06-20

申请号：US14833206

申请日：2015-08-24

Applicant: INVISTA North America SA.R.L.

Inventor： Eric Althoff ,  Nadia Kadi ,  Mihai Luchian Azoitei ,  Yih-En A Ban ,  Daniela Grabs-Röthlisberger ,  Alexander Pisarchik ,  Alexandre Zanghellini ,  Adriana L. Botes

IPC: C12N9/88 ,  A61K38/00 ,  C12P5/00 ,  C12P5/02 ,  C07C11/167 ,  C07C11/18 ,  C07C11/21 ,  C07K16/40 ,  C08F36/06 ,  C08F36/08 ,  C08F36/22 ,  G01N33/573 ,  C08F136/06 ,  C08F136/08

CPC classification number: C12N9/88 ,  A61K38/00 ,  C07C11/167 ,  C07C11/18 ,  C07C11/21 ,  C07K16/40 ,  C07K2299/00 ,  C08F36/06 ,  C08F36/08 ,  C08F36/22 ,  C08F136/06 ,  C08F136/08 ,  C12P5/007 ,  C12P5/026 ,  C12Y402/01053 ,  C12Y402/01095 ,  C12Y402/01127 ,  C12Y402/01131 ,  G01N33/573

Abstract: The present disclosure provides novel polypeptides with 3-buten-2-ol dehydratase activity, polypeptides with catalytic activity in the conversion of 3-methyl-3-buten-2-ol to isoprene, and crystal structure data for one of such polypeptides. Methods of making and using the polypeptides and their related crystal structure data are also provided.