公开(公告)号：US20070128608A1

公开(公告)日：2007-06-07

申请号：US10583457

申请日：2004-12-20

申请人： Kazunori Nakamura ,  Takahiro Kanagawa ,  Naohiro Noda ,  Satoshi Tsuneda ,  Hidenori Tani ,  Shinya Kurata

发明人： Kazunori Nakamura ,  Takahiro Kanagawa ,  Naohiro Noda ,  Satoshi Tsuneda ,  Hidenori Tani ,  Shinya Kurata

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6818 ,  Y10T436/143333 ,  C12Q2545/107

摘要： [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid. [Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.

摘要翻译： [问题]提供用于测定靶核酸的新型混合物，其特征在于能够进行核酸测定，同时：1）不需要稀释目标核酸的步骤; 2）不需要根据靶核酸的浓度改变探针浓度的程序。 [解决问题的方法] 1）包含一个内标核酸和两个用荧光染料标记的核酸探针的混合物; 2）用于测量Km值的混合物，其包含一个具有部分突变的内标核酸和一个用荧光染料标记的核酸探针; 3）混合物，其包含一个内标核酸和用两种荧光染料标记的一个双核酸探针; 以及通过其使用来测定核酸的方法。

公开(公告)号：US20110224417A1

公开(公告)日：2011-09-15

申请号：US13089420

申请日：2011-04-19

申请人： KAZUNORI NAKAMURA ,  TAKAHIRO KANAGAWA ,  NAOHIRO NODA ,  SATOSHI TSUNEDA ,  HIDENORI TANI ,  SHINYA KURATA

发明人： KAZUNORI NAKAMURA ,  TAKAHIRO KANAGAWA ,  NAOHIRO NODA ,  SATOSHI TSUNEDA ,  HIDENORI TANI ,  SHINYA KURATA

IPC分类号： C07H21/04

CPC分类号： C12Q1/6818 ,  Y10T436/143333 ,  C12Q2545/107

摘要： [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid.[Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.

摘要翻译： [问题]提供用于测定靶核酸的新型混合物，其特征在于能够进行核酸测定，同时：1）不需要稀释目标核酸的步骤; 2）不需要根据靶核酸的浓度改变探针浓度的程序。 [解决问题的方法] 1）包含一个内标核酸和两个用荧光染料标记的核酸探针的混合物; 2）用于测量Km值的混合物，其包含一个具有部分突变的内标核酸和一个用荧光染料标记的核酸探针; 3）混合物，其包含一个内标核酸和用两种荧光染料标记的一个双核酸探针; 以及通过其使用来测定核酸的方法。

公开(公告)号：US20110212442A1

公开(公告)日：2011-09-01

申请号：US12737567

申请日：2009-07-30

申请人： Yuji Sekiguchi ,  Naohiro Noda ,  Ryo Miyata ,  Kazunori Nakamura ,  Shinya Kurata ,  Satoshi Tsuneda ,  Hidenori Tani

发明人： Yuji Sekiguchi ,  Naohiro Noda ,  Ryo Miyata ,  Kazunori Nakamura ,  Shinya Kurata ,  Satoshi Tsuneda ,  Hidenori Tani

IPC分类号： C12Q1/68 ,  C07H21/00 ,  C07K2/00 ,  C07H1/00

CPC分类号： C12Q1/6876 ,  C12Q1/6816 ,  C12Q2565/107 ,  C12Q2527/107 ,  C12Q2525/101 ,  C12Q2563/107 ,  C12Q2537/125

摘要： A nucleic acid probe set includes (A) a fluorescent probe and (B) a binding probe. The fluorescent probe (A) is formed of an oligonucleotide, which includes (a) a nucleotide unit labeled with (d) a fluorescent substance. The binding probe (B) is formed of an oligonucleotide having (b1) a fluorescent probe binding region, which can hybridize to the fluorescent probe (A), and (b2) a target nucleic acid binding region, which can hybridize to a target nucleic acid sequence (C). The fluorescent substance (d) is a fluorescent substance which changes in fluorescent character upon interaction with guanine. At least one of nucleotide units which constitute the fluorescent probe (A) is an artificial nucleotide unit having a function to raise a dissociation temperature between the probe (A) and the fluorescent probe binding region (b1). The nucleic acid probe is provided with an improved fluorescence quenching efficiency.

摘要翻译： 核酸探针组包含（A）荧光探针和（B）结合探针。 荧光探针（A）由寡核苷酸形成，其包括（a）用（d）荧光物质标记的核苷酸单元。 结合探针（B）由具有（b1）可与荧光探针（A）杂交的荧光探针结合区域的寡核苷酸形成，（b2）可与靶核酸杂交的靶核酸结合区域 酸序列（C）。 荧光物质（d）是与鸟嘌呤相互作用时荧光特性变化的荧光物质。 构成荧光探针（A）的核苷酸单元中的至少一个是具有提高探针（A）和荧光探针结合区域（b1）之间的解离温度的功能的人造核苷酸单元。 核酸探针具有改善的荧光淬灭效率。

公开(公告)号：US20130065237A1

公开(公告)日：2013-03-14

申请号：US13665566

申请日：2012-10-31

申请人： Kazunori NAKAMURA ,  Takahiro Kanagawa ,  Naohiro Noda ,  Satoshi Tsuneda ,  Hidenori Tani ,  Shinya Kurata

发明人： Kazunori NAKAMURA ,  Takahiro Kanagawa ,  Naohiro Noda ,  Satoshi Tsuneda ,  Hidenori Tani ,  Shinya Kurata

IPC分类号： G01N21/64

CPC分类号： C12Q1/6818 ,  Y10T436/143333 ,  C12Q2545/107

摘要： [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid.[Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.

摘要翻译： [问题]提供用于测定靶核酸的新型混合物，其特征在于能够进行核酸测定，同时：1）不需要稀释目标核酸的步骤; 2）不需要根据靶核酸的浓度改变探针浓度的程序。 [解决问题的方法] 1）包含一个内标核酸和两个用荧光染料标记的核酸探针的混合物; 2）用于测量Km值的混合物，其包含一个具有部分突变的内标核酸和一个用荧光染料标记的核酸探针; 3）混合物，其包含一个内标核酸和用两种荧光染料标记的一个双核酸探针; 以及通过其使用来测定核酸的方法。

公开(公告)号：US20100015719A1

公开(公告)日：2010-01-21

申请号：US12540390

申请日：2009-08-13

申请人： Kazunori NAKAMURA ,  Takahiro Kanagawa ,  Naohiro Noda ,  Satoshi Tsuneda ,  Hidenori Tani ,  Shinya Kurata

发明人： Kazunori NAKAMURA ,  Takahiro Kanagawa ,  Naohiro Noda ,  Satoshi Tsuneda ,  Hidenori Tani ,  Shinya Kurata

IPC分类号： G01N33/00

CPC分类号： C12Q1/6818 ,  Y10T436/143333 ,  C12Q2545/107

摘要： [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid.[Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.

摘要翻译： [问题]提供用于测定靶核酸的新型混合物，其特征在于能够进行核酸测定，同时：1）不需要稀释目标核酸的步骤; 2）不需要根据靶核酸的浓度改变探针浓度的程序。 [解决问题的方法] 1）包含一个内标核酸和两个用荧光染料标记的核酸探针的混合物; 2）用于测量Km值的混合物，其包含一个具有部分突变的内标核酸和一个用荧光染料标记的核酸探针; 3）混合物，其包含一个内标核酸和用两种荧光染料标记的一个双核酸探针; 以及通过其使用来测定核酸的方法。

公开(公告)号：US07951604B2

公开(公告)日：2011-05-31

申请号：US12540390

申请日：2009-08-13

申请人： Kazunori Nakamura ,  Takahiro Kanagawa ,  Naohiro Noda ,  Satoshi Tsuneda ,  Hidenori Tani ,  Shinya Kurata

发明人： Kazunori Nakamura ,  Takahiro Kanagawa ,  Naohiro Noda ,  Satoshi Tsuneda ,  Hidenori Tani ,  Shinya Kurata

IPC分类号： G01N33/00 ,  G01N21/76 ,  C07H21/04

CPC分类号： C12Q1/6818 ,  Y10T436/143333 ,  C12Q2545/107

摘要： To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid.1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.

摘要翻译： 提供用于测定靶核酸的新型混合物，其特征在于能够进行核酸测定，同时：1）不需要稀释目标核酸的步骤; 2）不需要根据靶核酸的浓度改变探针浓度的程序。 1）包含一个内标核酸和两个用荧光染料标记的核酸探针的混合物; 2）用于测量Km值的混合物，其包含一个具有部分突变的内标核酸和一个用荧光染料标记的核酸探针; 3）混合物，其包含一个内标核酸和用两种荧光染料标记的一个双核酸探针; 以及通过其使用来测定核酸的方法。