发明申请
US20130065237A1 NOVEL MIXTURES FOR ASSAYING NUCLEIC ACID, NOVEL METHOD OF ASSAYING NUCLEIC ACID WITH THE USE OF THE SAME AND NUCLEIC ACID PROBE TO BE USED THEREFOR
有权
用于测定核酸的新型混合物，使用它们的核酸检测方法和使用其的核酸探针的新方法
- 专利标题： NOVEL MIXTURES FOR ASSAYING NUCLEIC ACID, NOVEL METHOD OF ASSAYING NUCLEIC ACID WITH THE USE OF THE SAME AND NUCLEIC ACID PROBE TO BE USED THEREFOR
- 专利标题（中）： 用于测定核酸的新型混合物，使用它们的核酸检测方法和使用其的核酸探针的新方法
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申请号： US13665566申请日： 2012-10-31
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公开(公告)号： US20130065237A1公开(公告)日： 2013-03-14
- 发明人: Kazunori NAKAMURA , Takahiro Kanagawa , Naohiro Noda , Satoshi Tsuneda , Hidenori Tani , Shinya Kurata
- 申请人： Kazunori NAKAMURA , Takahiro Kanagawa , Naohiro Noda , Satoshi Tsuneda , Hidenori Tani , Shinya Kurata
- 优先权： JP2003-423774 20031219
- 主分类号： G01N21/64
- IPC分类号： G01N21/64
摘要：
[Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid.[Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.
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