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公开(公告)号：US06812016B2
公开(公告)日：2004-11-02
申请号：US09919932
申请日：2001-08-02
申请人： Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
发明人： Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
IPC分类号： C12N914
摘要： The present invention relates to isolated polynucleotides which encode proteins with O-acetylhomoserine sulfhydrylase, vectors and cells containing the polynucleotides as well as methods of preparing L-amino acids.
摘要翻译： 本发明涉及用O-乙酰高丝氨酸巯基化酶编码蛋白质的分离的多核苷酸，含有多核苷酸的载体和细胞以及L-氨基酸的制备方法。
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公开(公告)号：US20050266535A1
公开(公告)日：2005-12-01
申请号：US11168476
申请日：2005-06-29
申请人： Brigitte Bathe , Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Michael Binder , Dieter Greissinger , Georg Thierbach , Alfred Puehler
发明人： Brigitte Bathe , Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Michael Binder , Dieter Greissinger , Georg Thierbach , Alfred Puehler
CPC分类号： C12N9/1007 , A23K20/142 , C07K14/34 , C12P13/12
摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metE gene is present in enhanced form, and the use of the polynucleotide sequences as hybridization probes.
摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，c）与a）或b）的多核苷酸互补的多核苷酸，以及d）至少包含至少 a），b）或c）的多核苷酸序列的15个连续核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metE基因以增强形式存在，以及使用多核苷酸 序列作为杂交探针。
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公开(公告)号：US20050074802A1
公开(公告)日：2005-04-07
申请号：US10936597
申请日：2004-09-09
申请人： Daniel Rey , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Brigitte Bathe , Klaus Huthmacher , Walter Pfefferle
发明人： Daniel Rey , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Brigitte Bathe , Klaus Huthmacher , Walter Pfefferle
IPC分类号： A23K10/12 , A23K20/142 , A23K40/10 , C07K14/34 , C12N1/21 , C12N15/31 , C12P13/12 , C12Q1/68 , C07H21/04 , C12N9/10 , C12N9/88 , C12N15/74
CPC分类号： C07K14/34 , A23K10/12 , A23K20/142 , A23K40/10 , C12P13/12
摘要： The invention relates to polynucleotides comprising polynucleotide sequences corresponding to the metD gene and parts thereof that encode polypeptide sequences and parts thereof possessing varying degrees of MetD transcription regulator activity, methods for preparation of L-amino acids, and methods of screening and amplifying polynucleotides encoding polypeptide sequences which comprise varying degrees of MetD transcription regulator activity. Further, the invention relates to animal food additives based on fermentation liquor and containing L-methionine, and to the preparation of such additive.
摘要翻译： 本发明涉及多核苷酸，其包含对应于metD基因的多核苷酸序列及其部分，其编码具有不同程度的MetD转录调节活性的多肽序列及其部分，用于制备L-氨基酸的方法，以及筛选和扩增编码多肽的多核苷酸的方法 包含不同程度的MetD转录调节活性的序列。 此外，本发明涉及基于发酵液并含有L-甲硫氨酸的动物性食品添加剂以及这种添加剂的制备。
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公开(公告)号：US06815196B2
公开(公告)日：2004-11-09
申请号：US09919831
申请日：2001-08-02
申请人： Brigitte Bathe , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
发明人： Brigitte Bathe , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
IPC分类号： C12N120
摘要： The present invention relates to isolated polynucleotides from Corynebacterium glutamicum encoding proteins of the transcription activator (MetR) and O-succinylhomoserine sulfhydrylase (MetZ). The invention also relates to producing L-amino acids, particularly methionine, in coryneform cells having attenuated metR and metZ genes.
摘要翻译： 本发明涉及编码转录激活子（MetR）和O-琥珀酰高丝氨酸巯基酸酶（MetZ）的蛋白质的谷氨酸棒杆菌分离的多核苷酸。 本发明还涉及在具有减毒的metR和metZ基因的棒状细胞中产生L-氨基酸，特别是甲硫氨酸。
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公开(公告)号：US06958228B2
公开(公告)日：2005-10-25
申请号：US09919891
申请日：2001-08-02
申请人： Brigitte Bathe , Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
发明人： Brigitte Bathe , Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
CPC分类号： C12N9/1007 , A23K20/142 , C12P13/12 , C12R1/15
摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metH gene is present in enhanced form, and use of the polynucleotide sequences as hybridization probes.
摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，c）与a）或b）的多核苷酸互补的多核苷酸，以及d）至少包含至少 a），b）或c）的多核苷酸序列的15个连续核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metH基因以增强形式存在，以及使用多核苷酸序列 作为杂交探针。
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公开(公告)号：US20050233373A1
公开(公告)日：2005-10-20
申请号：US11155656
申请日：2005-06-20
申请人： Brigitte Bathe , Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
发明人： Brigitte Bathe , Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
CPC分类号： C12N9/1007 , A23K20/142 , C12P13/12 , C12R1/15
摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metH gene is present in enhanced form, and use of the polynucleotide sequences as hybridization probes.
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7.发明授权Isolated polynucleotide from Corynebacterium encoding a homocysteine methyltransferase 有权来自编码高半胱氨酸甲基转移酶的棒状杆菌的分离的多核苷酸公开(公告)号：US06942996B2
公开(公告)日：2005-09-13
申请号：US09919835
申请日：2001-08-02
申请人： Brigitte Bathe , Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
发明人： Brigitte Bathe , Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
CPC分类号： C12N9/1007 , A23K20/142 , C07K14/34 , C12P13/12
摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID. No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and p1 d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metE gene is present in enhanced form, and the use of the polynucleotide sequences as hybridization probes.
摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID NO：1的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸。 b）编码包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽的多核苷酸，c）与a）或 b）和p1 d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metE基因是 以增强形式存在，以及使用多核苷酸序列作为杂交探针。
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公开(公告)号：US20050064551A1
公开(公告)日：2005-03-24
申请号：US10838245
申请日：2004-05-05
申请人： Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
发明人： Bettina Moeckel , Walter Pfefferle , Klaus Huthmacher , Christian Rueckert , Joern Kalinowski , Alfred Puehler , Michael Binder , Dieter Greissinger , Georg Thierbach
摘要： The present invention relates to isolated polynucleotides which encode proteins with O-acetylhomoserine sulfhydrylase, vectors and cells containing the polynucleotides as well as methods of preparing L-amino acids.
摘要翻译： 本发明涉及用O-乙酰高丝氨酸巯基化酶编码蛋白质的分离的多核苷酸，含有多核苷酸的载体和细胞以及L-氨基酸的制备方法。
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公开(公告)号：US07005175B2
公开(公告)日：2006-02-28
申请号：US10287463
申请日：2002-11-04
CPC分类号： B64C1/067 , Y10T428/13 , Y10T428/23 , Y10T428/238 , Y10T428/239 , Y10T428/24942
摘要： An aircraft fuselage shell is fabricated of a double-walled composite structural component, including a core element bonded and sandwiched between an inner cover layer and an outer cover layer. The inner cover layer forms the main load bearing fuselage inner skin, and the outer cover layer forms the fuselage outer skin which carries a smaller proportion of the loads. The core element may consist of fiber-reinforced composite material while the cover layers may consist of an aluminum alloy, another metal, or fiber-reinforced composite material. The core element is air permeable and has air flow channels extending longitudinally therethrough in at least one direction. The cover layers do not seal or enclose the core element, so the core element is ventilatable. A ventilating airflow carries moisture as water vapor out of the core element, and then the moisture is condensed and removed by a moisture management unit.
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