公开(公告)号：US20050266535A1

公开(公告)日：2005-12-01

申请号：US11168476

申请日：2005-06-29

申请人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach ,  Alfred Puehler

发明人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach ,  Alfred Puehler

IPC分类号： A23K1/16 ,  C07K14/34 ,  C12N9/10 ,  C12P13/12 ,  C12P13/04 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12N9/1007 ,  A23K20/142 ,  C07K14/34 ,  C12P13/12

摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metE gene is present in enhanced form, and the use of the polynucleotide sequences as hybridization probes.

摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，c）与a）或b）的多核苷酸互补的多核苷酸，以及d）至少包含至少 a），b）或c）的多核苷酸序列的15个连续核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metE基因以增强形式存在，以及使用多核苷酸 序列作为杂交探针。

公开(公告)号：US5633154A

公开(公告)日：1997-05-27

申请号：US336069

申请日：1994-11-04

申请人： Andreas Schaefer ,  Anna-Hildegard Seep-Feldhaus ,  Wolfgang Jaeger ,  Joern Kalinowski ,  Wolfgang Wohlleben ,  Alfred Puehler

发明人： Andreas Schaefer ,  Anna-Hildegard Seep-Feldhaus ,  Wolfgang Jaeger ,  Joern Kalinowski ,  Wolfgang Wohlleben ,  Alfred Puehler

IPC分类号： C12N15/09 ,  C07K14/34 ,  C12N1/21 ,  C12N9/10 ,  C12N15/11 ,  C12N15/77 ,  C12Q1/68 ,  C12R1/15

CPC分类号： C12N9/1055 ,  C07K14/34 ,  C12N15/11 ,  C12N15/77 ,  C12Q1/68 ,  C12Q1/689 ,  Y10S435/843

摘要： A method of locating insertion elements (IS elements) or transposons in coryneform bacteria, a positive selection system suitable for the above, the IS elements found in this manner and their use, is disclosed. The method involves:(1) The construction of a non-self-transferrable vector mobilizable from an E. coli mobilizer strain which vector is composed of(a) A DNA segment containing a replicon functional in E. coli,(b) A second DNA segment containing the DNA fragment coding for the mobilization function (Mob site containing the oriT),(c) A third DNA segment which recombines homologously in Gram-positive bacteria and/or contains a replicon functional in coryneform bacteria,(d) A DNA segment from Bacillus subtilis containing the sacB gene,(2) Transfer of this vector by means of conjugative transfer into the coryneform recipient strains,(3) Cultivation of the transconjugants containing the vector in an .about.10% sucrose-containing nutrient medium,(4) Lysis of the sucrose-resistant clones, cleaving of the plasmids with restriction endonucleases and analysis of the fragments.

摘要翻译： 公开了一种在棒状细菌中定位插入元件（IS元件）或转座子的方法，适用于上述的正选择系统，以这种方式发现的IS元件及其用途。 该方法包括：（1）从大肠杆菌动员菌株可移动的非自转型载体的构建，其载体由（a）在大肠杆菌中含有复制子功能的DNA片段组成，（b）第二 含有编码动员功能的DNA片段（含有oriT的Mob位点）的DNA片段，（c）在革兰氏阳性细菌中同源重组和/或含有棒状细菌功能性复制子的第三DNA片段，（d）DNA （2）通过共轭转移将该载体转移到棒状细胞受体菌株中，（3）在含有10％蔗糖的营养培养基中含有载体的转导结合体的培养物（4 ）分解蔗糖抗性克隆，用限制性内切核酸酶切割质粒并分析片段。

公开(公告)号：US06958228B2

公开(公告)日：2005-10-25

申请号：US09919891

申请日：2001-08-02

申请人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

发明人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： A23K1/16 ,  C12N9/10 ,  C12P13/12 ,  C07H21/04 ,  C12N1/20 ,  C12N15/09

CPC分类号： C12N9/1007 ,  A23K20/142 ,  C12P13/12 ,  C12R1/15

摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metH gene is present in enhanced form, and use of the polynucleotide sequences as hybridization probes.

摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，c）与a）或b）的多核苷酸互补的多核苷酸，以及d）至少包含至少 a），b）或c）的多核苷酸序列的15个连续核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metH基因以增强形式存在，以及使用多核苷酸序列 作为杂交探针。

公开(公告)号：US20050233373A1

公开(公告)日：2005-10-20

申请号：US11155656

申请日：2005-06-20

申请人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

发明人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： A23K1/16 ,  C12N9/10 ,  C12P13/12 ,  C12Q1/68 ,  C07H21/04 ,  C12N15/74 ,  C12P13/04

CPC分类号： C12N9/1007 ,  A23K20/142 ,  C12P13/12 ,  C12R1/15

摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metH gene is present in enhanced form, and use of the polynucleotide sequences as hybridization probes.

公开(公告)号：US06942996B2

公开(公告)日：2005-09-13

申请号：US09919835

申请日：2001-08-02

申请人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

发明人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： A23K1/16 ,  C07K14/34 ,  C12N9/10 ,  C12P13/12 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12N9/1007 ,  A23K20/142 ,  C07K14/34 ,  C12P13/12

摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID. No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and p1 d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metE gene is present in enhanced form, and the use of the polynucleotide sequences as hybridization probes.

摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID NO：1的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸。 b）编码包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽的多核苷酸，c）与a）或 b）和p1 d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metE基因是 以增强形式存在，以及使用多核苷酸序列作为杂交探针。

公开(公告)号：US20050064551A1

公开(公告)日：2005-03-24

申请号：US10838245

申请日：2004-05-05

申请人： Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

发明人： Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： C12N1/21 ,  C12N9/88 ,  C12P13/08 ,  C12P13/12 ,  C12N9/12 ,  C07H21/04 ,  C12N9/16 ,  C12N15/74

CPC分类号： C12P13/12 ,  C12N9/88 ,  C12P13/08

摘要： The present invention relates to isolated polynucleotides which encode proteins with O-acetylhomoserine sulfhydrylase, vectors and cells containing the polynucleotides as well as methods of preparing L-amino acids.

摘要翻译： 本发明涉及用O-乙酰高丝氨酸巯基化酶编码蛋白质的分离的多核苷酸，含有多核苷酸的载体和细胞以及L-氨基酸的制备方法。

公开(公告)号：US20050074802A1

公开(公告)日：2005-04-07

申请号：US10936597

申请日：2004-09-09

申请人： Daniel Rey ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Brigitte Bathe ,  Klaus Huthmacher ,  Walter Pfefferle

发明人： Daniel Rey ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Brigitte Bathe ,  Klaus Huthmacher ,  Walter Pfefferle

IPC分类号： A23K10/12 ,  A23K20/142 ,  A23K40/10 ,  C07K14/34 ,  C12N1/21 ,  C12N15/31 ,  C12P13/12 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/74

CPC分类号： C07K14/34 ,  A23K10/12 ,  A23K20/142 ,  A23K40/10 ,  C12P13/12

摘要： The invention relates to polynucleotides comprising polynucleotide sequences corresponding to the metD gene and parts thereof that encode polypeptide sequences and parts thereof possessing varying degrees of MetD transcription regulator activity, methods for preparation of L-amino acids, and methods of screening and amplifying polynucleotides encoding polypeptide sequences which comprise varying degrees of MetD transcription regulator activity. Further, the invention relates to animal food additives based on fermentation liquor and containing L-methionine, and to the preparation of such additive.

摘要翻译： 本发明涉及多核苷酸，其包含对应于metD基因的多核苷酸序列及其部分，其编码具有不同程度的MetD转录调节活性的多肽序列及其部分，用于制备L-氨基酸的方法，以及筛选和扩增编码多肽的多核苷酸的方法 包含不同程度的MetD转录调节活性的序列。 此外，本发明涉及基于发酵液并含有L-甲硫氨酸的动物性食品添加剂以及这种添加剂的制备。

公开(公告)号：US06815196B2

公开(公告)日：2004-11-09

申请号：US09919831

申请日：2001-08-02

申请人： Brigitte Bathe ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

发明人： Brigitte Bathe ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： C12N120

CPC分类号： C12N9/88 ,  C07K14/34 ,  C12P13/08 ,  C12P13/12

摘要： The present invention relates to isolated polynucleotides from Corynebacterium glutamicum encoding proteins of the transcription activator (MetR) and O-succinylhomoserine sulfhydrylase (MetZ). The invention also relates to producing L-amino acids, particularly methionine, in coryneform cells having attenuated metR and metZ genes.

摘要翻译： 本发明涉及编码转录激活子（MetR）和O-琥珀酰高丝氨酸巯基酸酶（MetZ）的蛋白质的谷氨酸棒杆菌分离的多核苷酸。 本发明还涉及在具有减毒的metR和metZ基因的棒状细胞中产生L-氨基酸，特别是甲硫氨酸。

公开(公告)号：US06812016B2

公开(公告)日：2004-11-02

申请号：US09919932

申请日：2001-08-02

申请人： Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

发明人： Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： C12N914

CPC分类号： C12P13/12 ,  C12N9/88 ,  C12P13/08

摘要： The present invention relates to isolated polynucleotides which encode proteins with O-acetylhomoserine sulfhydrylase, vectors and cells containing the polynucleotides as well as methods of preparing L-amino acids.

摘要翻译： 本发明涉及用O-乙酰高丝氨酸巯基化酶编码蛋白质的分离的多核苷酸，含有多核苷酸的载体和细胞以及L-氨基酸的制备方法。

公开(公告)号：US5380657A

公开(公告)日：1995-01-10

申请号：US33320

申请日：1993-03-18

申请人： Andreas Schaefer ,  Anna-Hildegard Seep-Feldhaus ,  Wolfgang Jaeger ,  Joern Kalinowski ,  Wolfgang Wohlleben ,  Alfred Puehler

发明人： Andreas Schaefer ,  Anna-Hildegard Seep-Feldhaus ,  Wolfgang Jaeger ,  Joern Kalinowski ,  Wolfgang Wohlleben ,  Alfred Puehler

IPC分类号： C12N15/09 ,  C07K14/34 ,  C12N1/21 ,  C12N9/10 ,  C12N15/11 ,  C12N15/77 ,  C12Q1/68 ,  C12R1/15

CPC分类号： C12N9/1055 ,  C07K14/34 ,  C12N15/11 ,  C12N15/77 ,  C12Q1/68 ,  C12Q1/689 ,  Y10S435/843

摘要： A method locating insertion elements (IS elements) or transposons in coryneform bacteria, a positive selection system suitable for the above, the IS elements found in this manner and their use, is disclosed. The method involves:(1) The construction of a non-self-transferrable vector mobilizable from an E. coli mobilizer strain which vector is composed of(a) A DNA segment containing a replicon functional in E. coli,(b) A second DNA segment containing the DNA fragment coding for the mobilization function (Mob site containing the oriT),(c) A third DNA segment which recombines homologously in Gram-positive bacteria and/or contains a replicon functional in coryneform bacteria,(d) A DNA segment from Bacillus subtilis containing the sacB gens,(2) Transfer of this vector by means of conjugative transfer into the coryneform recipient strains,(3) Cultivation of the transconjugants containing the vector in an .about.10% sucrose-containing nutrient medium,(4) Lysis of the sucrose-resistant clones, cleaving of the plasmids with restriction endonucleases and analysis of the fragments.

摘要翻译： 公开了一种在棒状细菌中定位插入元件（IS元件）或转座子的方法，适用于上述的正选择系统，以这种方式发现的IS元件及其用途。 该方法包括：（1）从大肠杆菌动员菌株可移动的非自转型载体的构建，其载体由（a）在大肠杆菌中含有复制子功能的DNA片段组成，（b）第二 含有编码动员功能的DNA片段（含有oriT的Mob位点）的DNA片段，（c）在革兰氏阳性细菌中同源重组和/或含有棒状细菌功能性复制子的第三DNA片段，（d）DNA （2）通过共轭转移将该载体转移到棒状细胞受体菌株中，（3）在含有10％蔗糖的营养培养基中含有载体的转导结合体的培养物（4 ）分解蔗糖抗性克隆，用限制性内切核酸酶切割质粒并分析片段。