公开(公告)号：US08961764B2

公开(公告)日：2015-02-24

申请号：US13273947

申请日：2011-10-14

Applicant: Peter Karl Trost ,  Michael E. Egan ,  Doug South ,  Brian E. Root ,  Orion N. Scott ,  James P. Landers

Inventor： Peter Karl Trost ,  Michael E. Egan ,  Doug South ,  Brian E. Root ,  Orion N. Scott ,  James P. Landers

IPC: G01N27/447 ,  G01N27/453 ,  G01N30/74 ,  G01N21/64 ,  G01J3/02 ,  G01J3/18 ,  G01N21/03

CPC classification number: G01N21/645 ,  G01J3/0218 ,  G01J3/18 ,  G01N21/6452 ,  G01N27/44721 ,  G01N2021/0346 ,  G01N2021/6484

Abstract: A DNA analyzer includes an interface for coupling a microfluidic chip to the DNA analyzer. The microfluidic chip includes a first separation channel for electrophoretic separation of DNA fragments in a first sample. Further, the DNA analyzer includes a first optical device. The first optical device includes an illuminating path and a detecting path. The illuminating path directs a first input light beam received from a light source to a first separation channel of the microfluidic chip. The first input light beam causes fluorescent labels attached on DNA fragments in the first separation channel to emit a first fluorescence light. The detecting path collects and directs the first fluorescent light to a first plurality of optical fibers. Further, the DNA analyzer includes a spectrometer configured to receive the first fluorescent light from the plurality of optical fibers and detect fluorescent components in the first fluorescent light.

Abstract translation: DNA分析仪包括用于将微流控芯片耦合到DNA分析仪的界面。 微流体芯片包括用于在第一样品中电泳分离DNA片段的第一分离通道。 此外，DNA分析仪包括第一光学装置。 第一光学装置包括照明路径和检测路径。 照明路径将从光源接收的第一输入光束引导到微流体芯片的第一分离通道。 第一输入光束使得附着在第一分离通道中的DNA片段上的荧光标记发射第一荧光。 检测路径将第一荧光收集并引导到第一多根光纤。 此外，DNA分析仪包括配置为从多根光纤接收第一荧光并且检测第一荧光中的荧光成分的光谱仪。

公开(公告)号：US20110229897A1

公开(公告)日：2011-09-22

申请号：US13064091

申请日：2011-03-04

Applicant: H.Randall Bell ,  John W. Pettit ,  James P. Landers ,  Joan M. Bienvenue ,  Daniel J. Marchiarullo ,  Brian E. Root ,  Orion N. Scott

Inventor： H.Randall Bell ,  John W. Pettit ,  James P. Landers ,  Joan M. Bienvenue ,  Daniel J. Marchiarullo ,  Brian E. Root ,  Orion N. Scott

IPC: C12Q1/68 ,  C12M1/34

CPC classification number: B01L3/502753 ,  B01L7/525 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0421 ,  G01N27/44791 ,  G01N29/32 ,  G01N2021/4186

Abstract: Aspects of the disclosure provides a DNA analyzer to facilitate an integrated single-chip DNA analysis. The DNA analyzer includes an interface for coupling a microfluidic chip to the DNA analyzer. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, and a second domain fluidically coupled to the first domain to receive the DNA fragments and perform electrophoretic separation of the DNA fragments. The DNA fragments are tagged with fluorescent labels. The DNA analyzer includes a detection module to excite the fluorescent labels to emit fluorescence and detect the emitted fluorescence. The detection module includes a laser source, a set of optical elements, a filter module and a photo-detector.

Abstract translation: 本公开的方面提供了DNA分析仪以促进集成的单芯片DNA分析。 DNA分析仪包括用于将微流控芯片耦合到DNA分析仪的界面。 微流体芯片包括构建用于DNA片段的聚合酶链反应（PCR）扩增的第一结构域和与第一结构域流体连接以接收DNA片段并进行DNA片段的电泳分离的第二结构域。 DNA片段用荧光标记物标记。 DNA分析仪包括用于激发荧光标记物以发出荧光并检测发出的荧光的检测模块。 检测模块包括激光源，一组光学元件，滤光器模块和光电检测器。

公开(公告)号：US09067207B2

公开(公告)日：2015-06-30

申请号：US13064091

申请日：2011-03-04

Applicant: H. Randall Bell ,  John W Pettit ,  James P Landers ,  Joan M Bienvenue ,  Daniel J Marchiarullo ,  Brian E Root ,  Orion N Scott

Inventor： H. Randall Bell ,  John W Pettit ,  James P Landers ,  Joan M Bienvenue ,  Daniel J Marchiarullo ,  Brian E Root ,  Orion N Scott

IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00 ,  G01N27/447

CPC classification number: B01L3/502753 ,  B01L7/525 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0421 ,  G01N27/44791 ,  G01N29/32 ,  G01N2021/4186

Abstract: Aspects of the disclosure provides a DNA analyzer to facilitate an integrated single-chip DNA analysis. The DNA analyzer includes an interface for coupling a microfluidic chip to the DNA analyzer. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, and a second domain fluidically coupled to the first domain to receive the DNA fragments and perform electrophoretic separation of the DNA fragments. The DNA fragments are tagged with fluorescent labels. The DNA analyzer includes a detection module to excite the fluorescent labels to emit fluorescence and detect the emitted fluorescence. The detection module includes a laser source, a set of optical elements, a filter module and a photo-detector.

Abstract translation: 本公开的方面提供了DNA分析仪以促进集成的单芯片DNA分析。 DNA分析仪包括用于将微流控芯片耦合到DNA分析仪的界面。 微流体芯片包括构建用于DNA片段的聚合酶链式反应（PCR）扩增的第一结构域和与第一结构域流体连接以接收DNA片段并进行DNA片段的电泳分离的第二结构域。 DNA片段用荧光标记物标记。 DNA分析仪包括用于激发荧光标记物以发出荧光并检测发出的荧光的检测模块。 检测模块包括激光源，一组光学元件，滤光器模块和光电检测器。