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公开(公告)号：US20110065101A1
公开(公告)日：2011-03-17
申请号：US12659251
申请日：2010-03-02
CPC分类号： B01L3/502753 , B01L7/525 , B01L2300/0816 , B01L2300/087 , B01L2400/0421 , G01N27/44791 , G01N29/32 , G01N2021/4186
摘要： Aspects of the disclosure provide a microfluidic chip. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, and a second domain for electrophoretic separation. The first domain includes at least a first reaction reservoir designated for PCR amplification based on a first sample, and a second reaction reservoir designated for PCR amplification based on a second sample. The second domain includes at least a first separation unit coupled to the first reaction reservoir to received first amplified DNA fragments based on the first sample, and a second separation unit coupled to the second reaction reservoir to received second amplified DNA fragments based on the second sample. The first separation unit is configured to perform electrophoretic separation for the first amplified DNA fragments, and the second separation unit is configured to perform electrophoretic separation for the second amplified DNA fragments.
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公开(公告)号：US09067207B2
公开(公告)日：2015-06-30
申请号：US13064091
申请日：2011-03-04
申请人： H. Randall Bell , John W Pettit , James P Landers , Joan M Bienvenue , Daniel J Marchiarullo , Brian E Root , Orion N Scott
发明人： H. Randall Bell , John W Pettit , James P Landers , Joan M Bienvenue , Daniel J Marchiarullo , Brian E Root , Orion N Scott
IPC分类号： C12Q1/68 , B01L3/00 , B01L7/00 , G01N27/447
CPC分类号： B01L3/502753 , B01L7/525 , B01L2300/0816 , B01L2300/087 , B01L2400/0421 , G01N27/44791 , G01N29/32 , G01N2021/4186
摘要： Aspects of the disclosure provides a DNA analyzer to facilitate an integrated single-chip DNA analysis. The DNA analyzer includes an interface for coupling a microfluidic chip to the DNA analyzer. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, and a second domain fluidically coupled to the first domain to receive the DNA fragments and perform electrophoretic separation of the DNA fragments. The DNA fragments are tagged with fluorescent labels. The DNA analyzer includes a detection module to excite the fluorescent labels to emit fluorescence and detect the emitted fluorescence. The detection module includes a laser source, a set of optical elements, a filter module and a photo-detector.
摘要翻译： 本公开的方面提供了DNA分析仪以促进集成的单芯片DNA分析。 DNA分析仪包括用于将微流控芯片耦合到DNA分析仪的界面。 微流体芯片包括构建用于DNA片段的聚合酶链式反应（PCR）扩增的第一结构域和与第一结构域流体连接以接收DNA片段并进行DNA片段的电泳分离的第二结构域。 DNA片段用荧光标记物标记。 DNA分析仪包括用于激发荧光标记物以发出荧光并检测发出的荧光的检测模块。 检测模块包括激光源，一组光学元件，滤光器模块和光电检测器。
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公开(公告)号：US20110070578A1
公开(公告)日：2011-03-24
申请号：US12659488
申请日：2010-03-10
CPC分类号： B01L3/502753 , B01L7/525 , B01L2300/0816 , B01L2300/087 , B01L2400/0421 , G01N27/44791 , G01N29/32 , G01N2021/4186
摘要： Aspects of the disclosure provide a microfluidic chip to facilitate DNA analysis. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, a dilution domain coupled to the first domain to dilute a PCR mixture received from the first domain, and a second domain that is coupled to the dilution domain so as to receive the amplified DNA fragments. The second domain includes a separation channel that is configured to perform electrophoretic separation of the amplified DNA fragments. In addition, the disclosure provides a DNA analyzer to act on the microfluidic chip to perform an integrated single chip DNA analysis.
摘要翻译： 本公开的方面提供了用于促进DNA分析的微流体芯片。 微流体芯片包括构建用于DNA片段的聚合酶链式反应（PCR）扩增的第一结构域，与第一结构域偶联的稀释结构域以稀释从第一结构域接收的PCR混合物，以及偶联至稀释结构域的第二结构域 以便接收扩增的DNA片段。 第二结构域包括被配置为进行经扩增的DNA片段的电泳分离的分离通道。 此外，本公开提供了一种DNA分析仪来作用于微流体芯片以进行集成的单芯片DNA分析。
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公开(公告)号：US09656261B2
公开(公告)日：2017-05-23
申请号：US13064094
申请日：2011-03-04
申请人： H. Randall Bell , Joan M Bienvenue , John W Pettit , James P Landers , Jessica V Norris , Orion N Scott , Daniel J Marchiarullo , Daniel C Leslie
发明人： H. Randall Bell , Joan M Bienvenue , John W Pettit , James P Landers , Jessica V Norris , Orion N Scott , Daniel J Marchiarullo , Daniel C Leslie
CPC分类号： B01L3/502753 , B01L7/525 , B01L2300/0816 , B01L2300/087 , B01L2400/0421 , G01N27/44791 , G01N29/32 , G01N2021/4186
摘要： Aspects of the disclosure provide a microfluidic chip to facilitate DNA analysis. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, a dilution domain coupled to the first domain to dilute a PCR mixture received from the first domain, and a second domain that is coupled to the dilution domain so as to receive the amplified DNA fragments. The second domain includes a separation channel that is configured to perform electrophoretic separation of the amplified DNA fragments. In addition, the disclosure provides a DNA analyzer to act on the microfluidic chip to perform an integrated single chip DNA analysis.
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公开(公告)号：US20110229898A1
公开(公告)日：2011-09-22
申请号：US13064094
申请日：2011-03-04
申请人： H. Randall Bell , Joan M. Bienvenue , John W. Pettit , James P. Landers , Jessica V. Norris , Orion N. Scott , Daniel J. Marchiarullo , Daniel C. Leslie
发明人： H. Randall Bell , Joan M. Bienvenue , John W. Pettit , James P. Landers , Jessica V. Norris , Orion N. Scott , Daniel J. Marchiarullo , Daniel C. Leslie
CPC分类号： B01L3/502753 , B01L7/525 , B01L2300/0816 , B01L2300/087 , B01L2400/0421 , G01N27/44791 , G01N29/32 , G01N2021/4186
摘要： Aspects of the disclosure provide a microfluidic chip to facilitate DNA analysis. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, a dilution domain coupled to the first domain to dilute a PCR mixture received from the first domain, and a second domain that is coupled to the dilution domain so as to receive the amplified DNA fragments. The second domain includes a separation channel that is configured to perform electrophoretic separation of the amplified DNA fragments. In addition, the disclosure provides a DNA analyzer to act on the microfluidic chip to perform an integrated single chip DNA analysis.
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