公开(公告)号：US20120090996A1

公开(公告)日：2012-04-19

申请号：US13273947

申请日：2011-10-14

Applicant: Peter Karl TROST ,  Michael E. EGAN ,  Doug SOUTH

Inventor： Peter Karl TROST ,  Michael E. EGAN ,  Doug SOUTH

IPC: G01N27/447

CPC classification number: G01N21/645 ,  G01J3/0218 ,  G01J3/18 ,  G01N21/6452 ,  G01N27/44721 ,  G01N2021/0346 ,  G01N2021/6484

Abstract: A DNA analyzer includes an interface for coupling a microfluidic chip to the DNA analyzer. The microfluidic chip includes a first separation channel for electrophoretic separation of DNA fragments in a first sample. Further, the DNA analyzer includes a first optical device. The first optical device includes an illuminating path and a detecting path. The illuminating path directs a first input light beam received from a light source to a first separation channel of the microfluidic chip. The first input light beam causes fluorescent labels attached on DNA fragments in the first separation channel to emit a first fluorescence light. The detecting path collects and directs the first fluorescent light to a first plurality of optical fibers. Further, the DNA analyzer includes a spectrometer configured to receive the first fluorescent light from the plurality of optical fibers and detect fluorescent components in the first fluorescent light.

Abstract translation: DNA分析仪包括用于将微流控芯片耦合到DNA分析仪的界面。 微流体芯片包括用于在第一样品中电泳分离DNA片段的第一分离通道。 此外，DNA分析仪包括第一光学装置。 第一光学装置包括照明路径和检测路径。 照明路径将从光源接收的第一输入光束引导到微流体芯片的第一分离通道。 第一输入光束使得附着在第一分离通道中的DNA片段上的荧光标记发射第一荧光。 检测路径将第一荧光收集并引导到第一多根光纤。 此外，DNA分析仪包括配置为从多根光纤接收第一荧光并检测第一荧光中的荧光成分的光谱仪。

公开(公告)号：US08961764B2

公开(公告)日：2015-02-24

申请号：US13273947

申请日：2011-10-14

Applicant: Peter Karl Trost ,  Michael E. Egan ,  Doug South ,  Brian E. Root ,  Orion N. Scott ,  James P. Landers

Inventor： Peter Karl Trost ,  Michael E. Egan ,  Doug South ,  Brian E. Root ,  Orion N. Scott ,  James P. Landers

IPC: G01N27/447 ,  G01N27/453 ,  G01N30/74 ,  G01N21/64 ,  G01J3/02 ,  G01J3/18 ,  G01N21/03

CPC classification number: G01N21/645 ,  G01J3/0218 ,  G01J3/18 ,  G01N21/6452 ,  G01N27/44721 ,  G01N2021/0346 ,  G01N2021/6484

Abstract: A DNA analyzer includes an interface for coupling a microfluidic chip to the DNA analyzer. The microfluidic chip includes a first separation channel for electrophoretic separation of DNA fragments in a first sample. Further, the DNA analyzer includes a first optical device. The first optical device includes an illuminating path and a detecting path. The illuminating path directs a first input light beam received from a light source to a first separation channel of the microfluidic chip. The first input light beam causes fluorescent labels attached on DNA fragments in the first separation channel to emit a first fluorescence light. The detecting path collects and directs the first fluorescent light to a first plurality of optical fibers. Further, the DNA analyzer includes a spectrometer configured to receive the first fluorescent light from the plurality of optical fibers and detect fluorescent components in the first fluorescent light.

Abstract translation: DNA分析仪包括用于将微流控芯片耦合到DNA分析仪的界面。 微流体芯片包括用于在第一样品中电泳分离DNA片段的第一分离通道。 此外，DNA分析仪包括第一光学装置。 第一光学装置包括照明路径和检测路径。 照明路径将从光源接收的第一输入光束引导到微流体芯片的第一分离通道。 第一输入光束使得附着在第一分离通道中的DNA片段上的荧光标记发射第一荧光。 检测路径将第一荧光收集并引导到第一多根光纤。 此外，DNA分析仪包括配置为从多根光纤接收第一荧光并且检测第一荧光中的荧光成分的光谱仪。