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    SPATIALLY RESOLVED SURFACE CAPTURE OF NUCLEIC ACIDS
    2.
    发明公开
    SPATIALLY RESOLVED SURFACE CAPTURE OF NUCLEIC ACIDS 审中-公开

    公开(公告)号：US20240052398A1

    公开(公告)日：2024-02-15

    申请号：US18450200

    申请日：2023-08-15

    申请人： Element Biosciences, Inc.

    发明人： Michael PREVITE ,  Shawn LEVY

    IPC分类号： C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6844

    CPC分类号： C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6844

    摘要： The present disclosure provides compositions, apparatuses and methods for capturing on a support nucleic acids from cellular samples, preparing library molecules on the support, amplifying the library molecules on the support to generate nucleic acid template molecules, and analyzing the immobilized nucleic acid template molecules including detecting and/or sequencing the immobilized nucleic acid template molecules. The immobilized nucleic acid template molecules correspond to the nucleic acids from the cellular samples. The immobilized nucleic acid template molecules are spatially located on the support at positions that correspond to the spatial location of the nucleic acids from the cellular sample.

    Compositions and methods for pairwise sequencing
    7.
    发明授权
    Compositions and methods for pairwise sequencing 有权

    公开(公告)号：US11535892B1

    公开(公告)日：2022-12-27

    申请号：US17377279

    申请日：2021-07-15

    申请人： Element Biosciences, Inc.

    发明人： Sinan Arslan ,  Junhua Zhao ,  Molly He ,  Samantha Snow ,  William Light ,  Matthew Kellinger ,  Michael Previte ,  Michael Kim ,  Hua Yu ,  Yu-Hsien Hwang-Fu ,  Marco Tjioe ,  Andrew Boddicker

    IPC分类号： C12Q1/6853 ,  C12Q1/6874

    摘要： The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.

    SINGLE-PASS PRIMARY ANALYSIS
    8.
    发明申请
    SINGLE-PASS PRIMARY ANALYSIS 有权

    公开(公告)号：US20220405523A1

    公开(公告)日：2022-12-22

    申请号：US17854042

    申请日：2022-06-30

    申请人： Element Biosciences, Inc.

    发明人： Chunhong ZHOU ,  Semyon KRUGLYAK ,  Francisco GARCIA ,  Minghao GUO ,  Haosen WANG ,  Ryan KELLY

    IPC分类号： G06K9/62 ,  G06T7/30 ,  G06V10/75 ,  G06V20/69

    摘要： Methods and systems for image analysis are provided, and in particular for identifying a set of base-calling locations in a flow cell for DNA sequencing. These include capturing flow cell images after each sequencing step performed on the flow cell, and identifying candidate cluster centers in at least one of the flow cell images. Intensities are determined for each candidate cluster center in a set of flow cell images. Purities are determined for each candidate cluster center based on the intensities. Each candidate cluster center with a purity greater than the purity of the surrounding candidate cluster centers within a distance threshold is added to a template set of base-calling locations.

    COMPOSITIONS AND METHODS FOR PAIRWISE SEQUENCING
    9.
    发明申请
    COMPOSITIONS AND METHODS FOR PAIRWISE SEQUENCING 有权

    公开(公告)号：US20220403445A1

    公开(公告)日：2022-12-22

    申请号：US17521239

    申请日：2021-11-08

    申请人： Element Biosciences, Inc.

    发明人： Sinan ARSLAN ,  Junhua ZHAO ,  Molly HE ,  Samantha SNOW ,  William LIGHT ,  Matthew KELLINGER ,  Michael PREVITE ,  Michael KIM ,  Hua YU ,  Yu-Hsien HWANG-FU ,  Marco TJIOE ,  Andrew BODDICKER

    IPC分类号： C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874

    摘要： The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.

    ENGINEERED POLYMERASES
    10.
    发明申请
    ENGINEERED POLYMERASES 有权

    公开(公告)号：US20220403351A1

    公开(公告)日：2022-12-22

    申请号：US17705011

    申请日：2022-03-25

    申请人： Element Biosciences, Inc.

    发明人： Mark Ambroso ,  Tyler Lopez ,  Michael Klein ,  Virginia Saade ,  Matthew Kellinger

    IPC分类号： C12N9/12 ,  C12Q1/6874

    摘要： Provided herein are engineered variants of archaeal, prokaryotic, and eukaryotic polymerases that exhibit enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, and improved uracil-tolerance, in polymerase-catalyzed nucleotide extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases, forming binding complexes and forming ternary complexes, and uses for conducting nucleic acid sequencing reactions.