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    Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA
    1.
    发明授权
    Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA 有权

    公开(公告)号：US10119150B2

    公开(公告)日：2018-11-06

    申请号：US14292317

    申请日：2014-05-30

    Applicant: Allele Biotechnology & Pharmaceuticals, Inc.

    Inventor： Jiwu Wang

    IPC: C12N15/85 ,  C12N5/074 ,  C12N15/11 ,  C07K14/47 ,  C07K14/475

    Abstract: The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.

    Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA
    4.
    发明授权
    Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA 有权

    公开(公告)号：US10155929B2

    公开(公告)日：2018-12-18

    申请号：US13893166

    申请日：2013-05-13

    Applicant: Allele Biotechnology and Pharmaceuticals, Inc.

    Inventor： Jiwu Wang ,  Luigi Warren ,  Yuhui Ni

    IPC: C12N5/074 ,  C12N15/11

    Abstract: The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.

    FEEDER-FREE DERIVATION OF HUMAN-INDUCED PLURIPOTENT STEM CELLS WITH SYNTHETIC MESSENGER RNA
    6.
    发明申请
    FEEDER-FREE DERIVATION OF HUMAN-INDUCED PLURIPOTENT STEM CELLS WITH SYNTHETIC MESSENGER RNA 审中-公开
    免疫衍生的人类诱导的具有合成信使RNA的细胞干细胞

    公开(公告)号：US20140349401A1

    公开(公告)日：2014-11-27

    申请号：US14292317

    申请日：2014-05-30

    Applicant: Allele Biotechnology & Pharmaceuticals, Inc.

    Inventor： Jiwu WANG

    IPC: C12N15/85

    CPC classification number: C12N15/85 ,  C07K14/4702 ,  C07K14/475 ,  C07K2319/01 ,  C12N5/0696 ,  C12N15/111 ,  C12N2310/14 ,  C12N2320/30 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/605 ,  C12N2501/608 ,  C12N2506/1307 ,  C12N2510/00

    Abstract: The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.

    Abstract translation: 本公开一般涉及通过动力学控制的过程使用工程化重编程因子来产生诱导多能干细胞（iPSC）的新方法和组合物。 具体地，本公开涉及建立重编程因子的组合，包括常规重编程因子与反式激活结构域之间的融合，优化用于重新编程各种类型的细胞。 更具体地，本文公开的示例性方法可用于从各种哺乳动物细胞类型（包括人成纤维细胞）产生诱导多能干细胞。 还公开了使用合成信使RNA的人诱导多能干细胞的无饲养物衍生的示例性方法。

    Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA
    7.
    发明授权
    Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA 有权

    公开(公告)号：US10435711B2

    公开(公告)日：2019-10-08

    申请号：US16181261

    申请日：2018-11-05

    Applicant: Allele Biotechnology & Pharmaceuticals, Inc.

    Inventor： Jiwu Wang

    IPC: C12N15/85 ,  C12N5/074 ,  C12N15/11 ,  C07K14/47 ,  C07K14/475

    Abstract: The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.

    Monomeric yellow-green fluorescent protein from cephalochordate
    8.
    发明授权
    Monomeric yellow-green fluorescent protein from cephalochordate 有权

    公开(公告)号：US10221221B2

    公开(公告)日：2019-03-05

    申请号：US13950239

    申请日：2013-07-24

    Applicant: Allele Biotechnology & Pharmaceuticals, Inc.

    Inventor： Nathan C. Shaner ,  Gerard G. Lambert ,  Yuhui Ni ,  Jiwu Wang

    IPC: A61K38/00 ,  C07K14/46 ,  G06F19/12 ,  G06F19/16 ,  C07K14/435

    Abstract: The present disclosure provides isolated nucleic acid sequences encoding a monomeric green/yellow fluorescent proteins, and fragments and derivatives thereof. Also provided is a method for engineering the nucleic acid sequence, a vector comprising the nucleic acid sequence, a host cell comprising the vector, and use of the vector in a method for expressing the nucleic acid sequence. The present invention further provides an isolated nucleic acid, or mimetic or complement thereof, that hybridizes under stringent conditions to the nucleic acid sequence. Additionally, the present invention provides a monomeric green/yellow fluorescent protein encoded by the nucleic acid sequence, as well as derivatives, fragments, and homologues thereof. Also provided is an antibody that specifically binds to the green/yellow fluorescent protein.

    Photoconvertible fluorescent proteins
    10.
    发明授权
    Photoconvertible fluorescent proteins 有权
    可转换荧光蛋白

    公开(公告)号：US09145447B2

    公开(公告)日：2015-09-29

    申请号：US12960397

    申请日：2010-12-03

    Applicant: Jiwu Wang ,  Robert Earl Campbell ,  Hiofan Hoi ,  Nathan Christopher Shaner

    Inventor： Jiwu Wang ,  Robert Earl Campbell ,  Hiofan Hoi ,  Nathan Christopher Shaner

    IPC: C12N5/14 ,  C12N5/16 ,  C07K14/435

    CPC classification number: C07K14/43595

    Abstract: The present disclosure provides an isolated nucleic acid sequence encoding a monomeric photoconvertible fluorescent protein, and fragments and derivatives thereof. Also provided is a method for engineering the nucleic acid sequence, a vector comprising the nucleic acid sequence, a host cell comprising the vector, and use of the vector in a method for expressing the nucleic acid sequence. The present disclosure further provides an isolated nucleic acid, or mimetic or complement thereof, that hybridizes under stringent conditions to the nucleic acid sequence. Additionally, the present provides a monomeric photoconvertible fluorescent protein encoded by the nucleic acid sequence, as well as derivatives, fragments, and homologues thereof. Also provided is an antibody that specifically binds to the photoconvertible fluorescent protein.

    Abstract translation: 本公开提供了编码单体可光转化荧光蛋白的分离的核酸序列及其片段和衍生物。 还提供了用于工程化核酸序列的方法，包含核酸序列的载体，包含载体的宿主细胞，以及用于表达核酸序列的方法中的载体的使用。 本公开进一步提供了在严格条件下与核酸序列杂交的分离的核酸或其模拟物或补体。 此外，本发明提供由核酸序列编码的单体可光转换荧光蛋白，以及其衍生物，片段和同系物。 还提供了特异性结合可光转化荧光蛋白的抗体。

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