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    Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA
    4.
    发明授权
    Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA 有权

    公开(公告)号：US10119150B2

    公开(公告)日：2018-11-06

    申请号：US14292317

    申请日：2014-05-30

    Applicant: Allele Biotechnology & Pharmaceuticals, Inc.

    Inventor： Jiwu Wang

    IPC: C12N15/85 ,  C12N5/074 ,  C12N15/11 ,  C07K14/47 ,  C07K14/475

    Abstract: The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.

    METHODS OF SELECTING ANTIBODIES AND ANTIBODY FRAGMENTS
    5.
    发明申请
    METHODS OF SELECTING ANTIBODIES AND ANTIBODY FRAGMENTS 审中-公开
    选择抗体和抗体片段的方法

    公开(公告)号：US20170044608A1

    公开(公告)日：2017-02-16

    申请号：US15212215

    申请日：2016-07-16

    Applicant: Allele Biotechnology & Pharmaceuticals, Inc.

    Inventor： Jiwu Wang ,  Gerald G. Lambert ,  Nathan C. Shaner

    IPC: C12Q1/68

    CPC classification number: C12Q1/6876 ,  G01N33/6854 ,  G01N33/6857

    Abstract: A multi-step method is disclosed for efficient selection of antibody or antibody fragments. In addition to the dramatically increased efficiency of finding antibodies against a given immunogen by the invented processes, the novel technology also differs from all previously known technologies in that the antibody or antibody fragments represent the most abundant antibodies induced by the immunogen, therefore having the highest potential of specificity and affinity. Utility of the invention can be found in virtually all areas that involve antibody or T cell receptor selection using any animal, against any immunogen.

    Abstract translation: 公开了用于有效选择抗体或抗体片段的多步法。 除了通过本发明的方法显着提高针对给定免疫原的抗体的效率之外，新技术还不同于所有先前已知的技术，因为抗体或抗体片段代表由免疫原诱导的最丰富的抗体，因此具有最高的 特异性和亲和力的潜力。 本发明的实用性可以在几乎所有涉及使用任何动物的抗体或T细胞受体选择的区域中针对任何免疫原发现。

    Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA
    6.
    发明授权
    Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA 有权

    公开(公告)号：US10435711B2

    公开(公告)日：2019-10-08

    申请号：US16181261

    申请日：2018-11-05

    Applicant: Allele Biotechnology & Pharmaceuticals, Inc.

    Inventor： Jiwu Wang

    IPC: C12N15/85 ,  C12N5/074 ,  C12N15/11 ,  C07K14/47 ,  C07K14/475

    Abstract: The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.

    Monomeric yellow-green fluorescent protein from cephalochordate
    7.
    发明授权
    Monomeric yellow-green fluorescent protein from cephalochordate 有权

    公开(公告)号：US10221221B2

    公开(公告)日：2019-03-05

    申请号：US13950239

    申请日：2013-07-24

    Applicant: Allele Biotechnology & Pharmaceuticals, Inc.

    Inventor： Nathan C. Shaner ,  Gerard G. Lambert ,  Yuhui Ni ,  Jiwu Wang

    IPC: A61K38/00 ,  C07K14/46 ,  G06F19/12 ,  G06F19/16 ,  C07K14/435

    Abstract: The present disclosure provides isolated nucleic acid sequences encoding a monomeric green/yellow fluorescent proteins, and fragments and derivatives thereof. Also provided is a method for engineering the nucleic acid sequence, a vector comprising the nucleic acid sequence, a host cell comprising the vector, and use of the vector in a method for expressing the nucleic acid sequence. The present invention further provides an isolated nucleic acid, or mimetic or complement thereof, that hybridizes under stringent conditions to the nucleic acid sequence. Additionally, the present invention provides a monomeric green/yellow fluorescent protein encoded by the nucleic acid sequence, as well as derivatives, fragments, and homologues thereof. Also provided is an antibody that specifically binds to the green/yellow fluorescent protein.

    FEEDER-FREE DERIVATION OF HUMAN-INDUCED PLURIPOTENT STEM CELLS WITH SYNTHETIC MESSENGER RNA
    9.
    发明申请
    FEEDER-FREE DERIVATION OF HUMAN-INDUCED PLURIPOTENT STEM CELLS WITH SYNTHETIC MESSENGER RNA 审中-公开
    免疫衍生的人类诱导的具有合成信使RNA的细胞干细胞

    公开(公告)号：US20140349401A1

    公开(公告)日：2014-11-27

    申请号：US14292317

    申请日：2014-05-30

    Applicant: Allele Biotechnology & Pharmaceuticals, Inc.

    Inventor： Jiwu WANG

    IPC: C12N15/85

    CPC classification number: C12N15/85 ,  C07K14/4702 ,  C07K14/475 ,  C07K2319/01 ,  C12N5/0696 ,  C12N15/111 ,  C12N2310/14 ,  C12N2320/30 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/605 ,  C12N2501/608 ,  C12N2506/1307 ,  C12N2510/00

    Abstract: The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.

    Abstract translation: 本公开一般涉及通过动力学控制的过程使用工程化重编程因子来产生诱导多能干细胞（iPSC）的新方法和组合物。 具体地，本公开涉及建立重编程因子的组合，包括常规重编程因子与反式激活结构域之间的融合，优化用于重新编程各种类型的细胞。 更具体地，本文公开的示例性方法可用于从各种哺乳动物细胞类型（包括人成纤维细胞）产生诱导多能干细胞。 还公开了使用合成信使RNA的人诱导多能干细胞的无饲养物衍生的示例性方法。

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