公开(公告)号：US09737472B2

公开(公告)日：2017-08-22

申请号：US14961678

申请日：2015-12-07

Applicant: ALLELE BIOTECHNOLOGY & PHARMACEUTICALS, INC.

Inventor： Jiwu Wang

IPC: A61K8/64 ,  A61C17/06 ,  A61K49/00 ,  A61K8/98 ,  C07K14/435 ,  C12N15/82

CPC classification number: A61K8/64 ,  A61C17/04 ,  A61K8/987 ,  A61K49/0021 ,  A61K49/0047 ,  A61K2800/434 ,  A61Q17/04 ,  C07K14/43504 ,  C09C2210/50 ,  C09J2489/00 ,  C12N15/8212 ,  C12N2310/3513

Abstract: The present disclosure relates to methods and compositions comprising naturally occurring light absorbing molecules for preventing damages from light exposure. Specific embodiments of this disclosure include fluorescent proteins from Brachiostoma lanceolatum.

公开(公告)号：US10119150B2

公开(公告)日：2018-11-06

申请号：US14292317

申请日：2014-05-30

Applicant: Allele Biotechnology & Pharmaceuticals, Inc.

Inventor： Jiwu Wang

IPC: C12N15/85 ,  C12N5/074 ,  C12N15/11 ,  C07K14/47 ,  C07K14/475

Abstract: The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.

公开(公告)号：US20170044608A1

公开(公告)日：2017-02-16

申请号：US15212215

申请日：2016-07-16

Applicant: Allele Biotechnology & Pharmaceuticals, Inc.

Inventor： Jiwu Wang ,  Gerald G. Lambert ,  Nathan C. Shaner

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  G01N33/6854 ,  G01N33/6857

Abstract: A multi-step method is disclosed for efficient selection of antibody or antibody fragments. In addition to the dramatically increased efficiency of finding antibodies against a given immunogen by the invented processes, the novel technology also differs from all previously known technologies in that the antibody or antibody fragments represent the most abundant antibodies induced by the immunogen, therefore having the highest potential of specificity and affinity. Utility of the invention can be found in virtually all areas that involve antibody or T cell receptor selection using any animal, against any immunogen.

Abstract translation: 公开了用于有效选择抗体或抗体片段的多步法。 除了通过本发明的方法显着提高针对给定免疫原的抗体的效率之外，新技术还不同于所有先前已知的技术，因为抗体或抗体片段代表由免疫原诱导的最丰富的抗体，因此具有最高的 特异性和亲和力的潜力。 本发明的实用性可以在几乎所有涉及使用任何动物的抗体或T细胞受体选择的区域中针对任何免疫原发现。

公开(公告)号：US10435711B2

公开(公告)日：2019-10-08

申请号：US16181261

申请日：2018-11-05

Applicant: Allele Biotechnology & Pharmaceuticals, Inc.

Inventor： Jiwu Wang

IPC: C12N15/85 ,  C12N5/074 ,  C12N15/11 ,  C07K14/47 ,  C07K14/475

Abstract: The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.

公开(公告)号：US10221221B2

公开(公告)日：2019-03-05

申请号：US13950239

申请日：2013-07-24

Applicant: Allele Biotechnology & Pharmaceuticals, Inc.

Inventor： Nathan C. Shaner ,  Gerard G. Lambert ,  Yuhui Ni ,  Jiwu Wang

IPC: A61K38/00 ,  C07K14/46 ,  G06F19/12 ,  G06F19/16 ,  C07K14/435

Abstract: The present disclosure provides isolated nucleic acid sequences encoding a monomeric green/yellow fluorescent proteins, and fragments and derivatives thereof. Also provided is a method for engineering the nucleic acid sequence, a vector comprising the nucleic acid sequence, a host cell comprising the vector, and use of the vector in a method for expressing the nucleic acid sequence. The present invention further provides an isolated nucleic acid, or mimetic or complement thereof, that hybridizes under stringent conditions to the nucleic acid sequence. Additionally, the present invention provides a monomeric green/yellow fluorescent protein encoded by the nucleic acid sequence, as well as derivatives, fragments, and homologues thereof. Also provided is an antibody that specifically binds to the green/yellow fluorescent protein.